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- W1999399044 abstract "The mitochondrial seryl-tRNA synthetase (mt SerRS) from Bos taurus was overexpressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion method. Crystals grew in a very narrow range of conditions using PEG 8000 as precipitant at room temperature. An appropriate concentration of lithium sulfate was critical for crystal nucleation. Crystals diffracted well beyond a resolution of 1.6 A and were found to belong to the orthorhombic space group C222(1), with unit-cell parameters a = 79.89, b = 230.42, c = 135.60 A. There is one dimer (M(r) approximately 113 kDa) in the asymmetric unit, with a solvent content of 55%. Efforts to solve the phase problem by molecular replacement are under way." @default.
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- W1999399044 date "2004-06-22" @default.
- W1999399044 modified "2023-10-16" @default.
- W1999399044 title "Crystallization and preliminary X-ray diffraction study of mammalian mitochondrial seryl-tRNA synthetase" @default.
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- W1999399044 doi "https://doi.org/10.1107/s0907444904011217" @default.
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