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- W1999444388 abstract "A gene homologous to Saccharomyces cerevisiae PMR1 has been cloned in the methylotrophic yeast Hansenula polymorpha. The partial DNA fragment of the H. polymorpha homologue was initially obtained by a polymerase chain reaction and used to isolate the entire gene which encodes a protein of 918 amino acids. The putative gene product contains all ten of the conserved regions observed in P-type ATPases. The cloned gene product exhibits 60·3% amino acid identity to the S. cerevisiae PMR1 gene product and complemented the growth defect of a S. cerevisiae pmr1 null mutant in the EGTA-containing medium. The results demonstrate that the H. polymorpha gene encodes the functional homologue of the S. cerevisiae PMR1 gene product, a P-type Ca2+-ATPase. The DNA sequence of the H. polymorpha homologue has been submitted to GenBank with the Accession Number U92083. © 1998 John Wiley & Sons, Ltd." @default.
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- W1999444388 date "1998-09-30" @default.
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- W1999444388 title "Cloning and characterization of theHansenula polymorpha homologue of theSaccharomyces cerevisiae PMR1 gene" @default.
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- W1999444388 doi "https://doi.org/10.1002/(sici)1097-0061(19980930)14:13<1233::aid-yea322>3.0.co;2-y" @default.
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