Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999452963> ?p ?o ?g. }
Showing items 1 to 56 of
56
with 100 items per page.
- W1999452963 endingPage "S409" @default.
- W1999452963 startingPage "S409" @default.
- W1999452963 abstract "Glucagon-like peptide-1 (GLP-1) is an insulinotropic protein. It was reported that the continuous infusion of GLP-1 normalized the blood glucose level in type 2 diabetes animal model. However, the short half-life of GLP-1 has limited its application to clinical settings and prompted us to develop a GLP-1 gene therapy system. Our previous results showed that the delivery of pb-GLP-1 using polyethylenimine (PEI) reduced the blood glucose level effectively. However, the glucose level was not completely normalized. In the present study, the more efficient GLP-1 expression system was developed using two step transcription amplification (TSTA). To evaluate the TSTA system, pb-Gal4-p65 and pUAS-Luc were constructed. The pUAS-Luc/pb-Gal4-p65 system showed the highest transfection efficiency at a 2:1 pUAS-Luc:pb-Gal4-p65 weight ratio. In addition, the transgene expression by the TSTA system was at least 4 times higher than pb-Luc. To apply the TSTA system to the GLP-1 expression plasmid, pUAS-GLP-1 was constructed. The pUAS-GLP-1/pb-Gal4-p65 system showed higher mRNA level than pb-GLP-1. In addition, the level of GLP-1 by the pUAS-GLP-1/pb-Gal4-p65 system was more than 4 times higher than pb-GLP-1. Therefore, the TSTA GLP-1 expression system may be useful to develop gene therapy system for type 2 diabetes." @default.
- W1999452963 created "2016-06-24" @default.
- W1999452963 date "2005-05-01" @default.
- W1999452963 modified "2023-10-18" @default.
- W1999452963 title "1060. An Efficient GLP-1 Expression System Using Two Step Transcription Amplification for Type 2 Diabetes Gene Therapy" @default.
- W1999452963 doi "https://doi.org/10.1016/j.ymthe.2005.07.607" @default.
- W1999452963 hasPublicationYear "2005" @default.
- W1999452963 type Work @default.
- W1999452963 sameAs 1999452963 @default.
- W1999452963 citedByCount "0" @default.
- W1999452963 crossrefType "journal-article" @default.
- W1999452963 hasBestOaLocation W19994529631 @default.
- W1999452963 hasConcept C104317684 @default.
- W1999452963 hasConcept C111599444 @default.
- W1999452963 hasConcept C134018914 @default.
- W1999452963 hasConcept C138885662 @default.
- W1999452963 hasConcept C150194340 @default.
- W1999452963 hasConcept C179926584 @default.
- W1999452963 hasConcept C2777180221 @default.
- W1999452963 hasConcept C41895202 @default.
- W1999452963 hasConcept C54355233 @default.
- W1999452963 hasConcept C555293320 @default.
- W1999452963 hasConcept C70721500 @default.
- W1999452963 hasConcept C86803240 @default.
- W1999452963 hasConceptScore W1999452963C104317684 @default.
- W1999452963 hasConceptScore W1999452963C111599444 @default.
- W1999452963 hasConceptScore W1999452963C134018914 @default.
- W1999452963 hasConceptScore W1999452963C138885662 @default.
- W1999452963 hasConceptScore W1999452963C150194340 @default.
- W1999452963 hasConceptScore W1999452963C179926584 @default.
- W1999452963 hasConceptScore W1999452963C2777180221 @default.
- W1999452963 hasConceptScore W1999452963C41895202 @default.
- W1999452963 hasConceptScore W1999452963C54355233 @default.
- W1999452963 hasConceptScore W1999452963C555293320 @default.
- W1999452963 hasConceptScore W1999452963C70721500 @default.
- W1999452963 hasConceptScore W1999452963C86803240 @default.
- W1999452963 hasLocation W19994529631 @default.
- W1999452963 hasOpenAccess W1999452963 @default.
- W1999452963 hasPrimaryLocation W19994529631 @default.
- W1999452963 hasRelatedWork W2009966535 @default.
- W1999452963 hasRelatedWork W2020486934 @default.
- W1999452963 hasRelatedWork W2034953144 @default.
- W1999452963 hasRelatedWork W2093805897 @default.
- W1999452963 hasRelatedWork W2111172999 @default.
- W1999452963 hasRelatedWork W2152866106 @default.
- W1999452963 hasRelatedWork W2171277769 @default.
- W1999452963 hasRelatedWork W2172226517 @default.
- W1999452963 hasRelatedWork W2945656281 @default.
- W1999452963 hasRelatedWork W4307000021 @default.
- W1999452963 hasVolume "11" @default.
- W1999452963 isParatext "false" @default.
- W1999452963 isRetracted "false" @default.
- W1999452963 magId "1999452963" @default.
- W1999452963 workType "article" @default.