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• W1999461765 endingPage "264" @default.
• W1999461765 startingPage "246" @default.
• W1999461765 abstract "The Dot/Icm type IV secretion system of Legionella pneumophila triggers robust activation of caspase-3 during early and exponential stages of proliferation within human macrophages, but apoptosis is delayed till late stages of infection, which is novel. As caspase-3 is the executioner of the cell, we tested the hypothesis that L. pneumophila triggers anti-apoptotic signalling within the infected human macrophages to halt caspase-3 from dismantling the cells. Here we show that during early and exponential replication, L. pneumophila-infected human monocyte-derived macrophages (hMDMs) exhibit a remarkable resistance to induction of apoptosis, in a Dot/Icm-dependent manner. Microarray analyses and real-time PCR reveal that during exponential intracellular replication, L. pneumophila triggers upregulation of 12 anti-apoptotic genes that are linked to activation of the nuclear transcription factor kappa-B (NF-kappaB). Our data show that L. pneumophila induces a Dot/Icm-dependent sustained nuclear translocation of the p50 and p65 subunits of NF-kappaB during exponential intracellular replication. Bacterial entry is essential both for the anti-apoptotic phenotype of infected hMDMs and for nuclear translocation of the p65. Using p65-/- and IKKalpha-/- beta-/- double knockout mouse embryonic fibroblast cell lines, we show that nuclear translocation of NF-kappaB is required for the resistance of L. pneumophila-infected cells to apoptosis-inducing agents. In addition, the L. pneumophila-induced nuclear translocation of NF-kappaB requires the activity of IKKalpha and/or IKKbeta. We conclude that although the Dot/Icm secretion system of L. pneumophila elicits an early robust activation of caspase-3 in human macrophages, it triggers a strong anti-apoptotic signalling cascade mediated, at least in part by NF-kappaB, which renders the cells refractory to external potent apoptotic stimuli." @default.
• W1999461765 created "2016-06-24" @default.
• W1999461765 creator A5018120874 @default.
• W1999461765 creator A5022965285 @default.
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• W1999461765 creator A5076113010 @default.
• W1999461765 creator A5083614444 @default.
• W1999461765 date "2006-08-15" @default.
• W1999461765 modified "2023-10-16" @default.
• W1999461765 title "Anti-apoptotic signalling by the Dot/Icm secretion system of<i>L. pneumophila</i>" @default.
• W1999461765 cites W1489373212 @default.
• W1999461765 cites W1492587973 @default.
• W1999461765 cites W1599900825 @default.
• W1999461765 cites W1827669359 @default.
• W1999461765 cites W1931230038 @default.
• W1999461765 cites W1946493814 @default.
• W1999461765 cites W1966176339 @default.
• W1999461765 cites W1970452315 @default.
• W1999461765 cites W1972836302 @default.
• W1999461765 cites W1973862406 @default.
• W1999461765 cites W1974405307 @default.
• W1999461765 cites W1976195783 @default.
• W1999461765 cites W1976382352 @default.
• W1999461765 cites W1976935037 @default.
• W1999461765 cites W1983398917 @default.
• W1999461765 cites W1984571968 @default.
• W1999461765 cites W1988004907 @default.
• W1999461765 cites W1988433390 @default.
• W1999461765 cites W1998786045 @default.
• W1999461765 cites W2000947843 @default.
• W1999461765 cites W2003890592 @default.
• W1999461765 cites W2004773897 @default.
• W1999461765 cites W2007412334 @default.
• W1999461765 cites W2008034134 @default.
• W1999461765 cites W2012193854 @default.
• W1999461765 cites W2016503643 @default.
• W1999461765 cites W2016512598 @default.
• W1999461765 cites W2018554367 @default.
• W1999461765 cites W2021455181 @default.
• W1999461765 cites W2021750140 @default.
• W1999461765 cites W2024746774 @default.
• W1999461765 cites W2030575711 @default.
• W1999461765 cites W2031161780 @default.
• W1999461765 cites W2032764487 @default.
• W1999461765 cites W2035088045 @default.
• W1999461765 cites W2039597975 @default.
• W1999461765 cites W2039629288 @default.
• W1999461765 cites W2041242716 @default.
• W1999461765 cites W2042087846 @default.
• W1999461765 cites W2043118554 @default.
• W1999461765 cites W2045789066 @default.
• W1999461765 cites W2048778098 @default.
• W1999461765 cites W2049427398 @default.
• W1999461765 cites W2053372168 @default.
• W1999461765 cites W2053434264 @default.
• W1999461765 cites W2060702462 @default.
• W1999461765 cites W2063495408 @default.
• W1999461765 cites W2064254181 @default.
• W1999461765 cites W2065079479 @default.
• W1999461765 cites W2066974855 @default.
• W1999461765 cites W2069356140 @default.
• W1999461765 cites W2069855944 @default.
• W1999461765 cites W2075821783 @default.
• W1999461765 cites W2076015888 @default.
• W1999461765 cites W2076796449 @default.
• W1999461765 cites W2080224726 @default.
• W1999461765 cites W2083829960 @default.
• W1999461765 cites W2083906562 @default.
• W1999461765 cites W2093140151 @default.
• W1999461765 cites W2093633496 @default.
• W1999461765 cites W2093725577 @default.
• W1999461765 cites W2094311333 @default.
• W1999461765 cites W2094346670 @default.
• W1999461765 cites W2099454764 @default.
• W1999461765 cites W2105562119 @default.
• W1999461765 cites W2106280019 @default.
• W1999461765 cites W2114404462 @default.
• W1999461765 cites W2118508881 @default.
• W1999461765 cites W2120492514 @default.
• W1999461765 cites W2122301597 @default.
• W1999461765 cites W2122931119 @default.
• W1999461765 cites W2124221273 @default.
• W1999461765 cites W2128926303 @default.
• W1999461765 cites W2130745002 @default.
• W1999461765 cites W2138231814 @default.
• W1999461765 cites W2138393269 @default.
• W1999461765 cites W2138820761 @default.
• W1999461765 cites W2144841919 @default.
• W1999461765 cites W2145006562 @default.
• W1999461765 cites W2147921405 @default.
• W1999461765 cites W2149025394 @default.
• W1999461765 cites W2155795106 @default.
• W1999461765 cites W2157066392 @default.
• W1999461765 cites W2157685329 @default.
• W1999461765 cites W2159722075 @default.
• W1999461765 cites W2160664668 @default.

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