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- W1999462829 abstract "A chromatographic method is described for the separation and determination of nucleotides, nucleosides, and purine and pyrimidine bases using Dowex 1-X8 resin columns 0.9 × 150 cm in length. Absorbancy at 260 and 280 mμ at optical path lengths of either 0.2 or 1.0 cm were continuously recorded using a modified double-beam spectrophotometer. To avoid changes in molar absorptivity which occur in many compounds with changing pH, elution was accomplished at one pH (4.4) by changing the concentration of sodium acetate from 0.15 to 3.0 M. The complete elution schedule required 28 hr. To shorten the time required for eluting the triphosphates, a 0.9 × 50 cm column operated at pH 3.6 was used which allowed UTP, ATP, and GTP to be determined in 6 hr." @default.
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- W1999462829 date "1963-08-01" @default.
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- W1999462829 title "Analytical techniques for cell fractions. III. Nucleotides and related compounds" @default.
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- W1999462829 doi "https://doi.org/10.1016/0003-2697(63)90106-4" @default.
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