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- W1999463370 abstract "Abstract Yeast cells growing in a low phosphate medium were labeled with a pulse of 32Pi or [3H]adenine and harvested after 15 minutes. Total RNA was extracted and digested with ribonuclease T1. Poly(A)-rich fragments were isolated from the digest by hybridization to poly(U) impregnated fiberglass filters. Gel filtration showed the fragments to have a uniform chain length of about sixteen. Analysis of the composition gave (A11, C4, U). Complete pancreatic ribonuclease and partial spleen phosphodiesterase digests gave the sequence of the 5′ end of the fragment as CpApApUp-. Since the fragment was a ribonuclease T1 product, the data points to a unique sequence of at least five residues, -GpCpApApUp-, adjacent to the poly(A)-rich terminus of pulse-labeled yeast mRNA. The remainder of the poly(A)-rich fragment consists of A residues with a few randomly interspaced C residues. The known specificity of yeast poly(A) polymerase can account for the presence of C residues in poly(A) tracts." @default.
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- W1999463370 date "1973-10-01" @default.
- W1999463370 modified "2023-09-23" @default.
- W1999463370 title "Unique nucleotide sequence adjacent to the poly(A) region in yeast RNA" @default.
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- W1999463370 doi "https://doi.org/10.1016/0006-291x(73)91171-6" @default.
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