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- W1999463375 abstract "The development of a convenient, low cost, low power He-Cd laser-induced fluorescence (LIF) detection system for HPLC is described. The system was developed for the detection of primary amines derivatized with naphthalenedialdehyde (NDA). The LIF system incorporates a fiber optic cell similar to the cell developed by Yeung and coworkers, which was modified in order to improve the S/Nratio. The LIF system exhibited detection limits of 0.75-1.2 fmol which was comparable to the detection limits obtained for the derivatized amino acids with a high power argon ion LIF system and demonstrated a tenfold improvement in comparison to a commercial fluorescence detector. In addition, the LIF cell, collection optics and photomultiplier tube were mounted on a standard laboratory ringstand, which allowed the system to be easily moved. The applicability of the LIF system was demonstrated by derivatizing a human tear sample with NDA and subjecting it to HPLC analysis with LIF detection. Several amino acids were identified in the resulting chromatogram." @default.
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- W1999463375 date "1989-02-01" @default.
- W1999463375 modified "2023-10-17" @default.
- W1999463375 title "High Sensitivity Fluorescence Detection of Naphthalenedialdehyde-Derivatized Amino Acids with a Low Power Helium-Cadmium Laser for High Performance Liquid Chromatographic Analysis" @default.
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- W1999463375 doi "https://doi.org/10.2116/analsci.5.23" @default.
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