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- W1999503050 endingPage "81" @default.
- W1999503050 startingPage "71" @default.
- W1999503050 abstract "Regulation of intracellular pH (pHi) and protection against cytosolic acidification is primarily a function of the ubiquitous plasma membrane Na+/H + exchanger-1 (NHE1), which uses a highly conserved process to transfer cytosolic hydrogen ions (H +) across plasma membranes in exchange for extracellular sodium ions (Na +). Growth factors, which are essential regulators of morphogenesis, have also been found to be key activators of NHE1 exchanger activity; however, the crosstalk between both has not been fully evaluated during organ development. Here we report that mammary branching morphogenesis induced by transforming growth factor-alpha (TGFα) requires PI3K-dependent NHE1-activation and subsequent pHi alkalization. Inhibiting NHE1 activity after TGFα stimulation with 10 μM of the NHE1-specific inhibitor N-Methyl-N-isobutyl Amiloride (MIA) dramatically disrupted branching morphogenesis, induced extensive proliferation, ectopic expression of the epithelial hyper-proliferative marker Keratin-6 and sustained activation of MAPK. Together these findings indicate a novel developmental signaling cascade involving TGFα > PI3K > NHE1 > pHi alkalization, which leads to a permissible environment for MAPK negative feedback inhibition and thus regulated mammary branching morphogenesis." @default.
- W1999503050 created "2016-06-24" @default.
- W1999503050 creator A5024976781 @default.
- W1999503050 creator A5030300405 @default.
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- W1999503050 creator A5056352891 @default.
- W1999503050 creator A5066003122 @default.
- W1999503050 creator A5090282528 @default.
- W1999503050 date "2012-05-01" @default.
- W1999503050 modified "2023-10-17" @default.
- W1999503050 title "Intracellular pH regulation by Na+/H+ exchanger-1 (NHE1) is required for growth factor-induced mammary branching morphogenesis" @default.
- W1999503050 cites W1469687879 @default.
- W1999503050 cites W1495654654 @default.
- W1999503050 cites W1503508769 @default.
- W1999503050 cites W1572775048 @default.
- W1999503050 cites W1578332600 @default.
- W1999503050 cites W1583309325 @default.
- W1999503050 cites W1584726943 @default.
- W1999503050 cites W1753190777 @default.
- W1999503050 cites W179174977 @default.
- W1999503050 cites W1902824914 @default.
- W1999503050 cites W1964151908 @default.
- W1999503050 cites W1964316807 @default.
- W1999503050 cites W1965009930 @default.
- W1999503050 cites W1971853480 @default.
- W1999503050 cites W1974504182 @default.
- W1999503050 cites W1975182932 @default.
- W1999503050 cites W1975246259 @default.
- W1999503050 cites W1977055673 @default.
- W1999503050 cites W1979564359 @default.
- W1999503050 cites W1981721404 @default.
- W1999503050 cites W1981813507 @default.
- W1999503050 cites W1983026183 @default.
- W1999503050 cites W1983768617 @default.
- W1999503050 cites W1988081857 @default.
- W1999503050 cites W1990580490 @default.
- W1999503050 cites W1992614445 @default.
- W1999503050 cites W1995612509 @default.
- W1999503050 cites W1998789156 @default.
- W1999503050 cites W2001870992 @default.
- W1999503050 cites W2005496979 @default.
- W1999503050 cites W2005734517 @default.
- W1999503050 cites W2007916146 @default.
- W1999503050 cites W2008877649 @default.
- W1999503050 cites W2009755318 @default.
- W1999503050 cites W2010040980 @default.
- W1999503050 cites W2010704664 @default.
- W1999503050 cites W2013507620 @default.
- W1999503050 cites W2016081191 @default.
- W1999503050 cites W2020372666 @default.
- W1999503050 cites W2024662178 @default.
- W1999503050 cites W2025800972 @default.
- W1999503050 cites W2028163303 @default.
- W1999503050 cites W2031928941 @default.
- W1999503050 cites W2033936610 @default.
- W1999503050 cites W2034356318 @default.
- W1999503050 cites W2039577556 @default.
- W1999503050 cites W2044731113 @default.
- W1999503050 cites W2045066549 @default.
- W1999503050 cites W2045545926 @default.
- W1999503050 cites W2047822976 @default.
- W1999503050 cites W2048296596 @default.
- W1999503050 cites W2049533803 @default.
- W1999503050 cites W2059249920 @default.
- W1999503050 cites W2060315853 @default.
- W1999503050 cites W2067806014 @default.
- W1999503050 cites W2071508050 @default.
- W1999503050 cites W2076139683 @default.
- W1999503050 cites W2084441235 @default.
- W1999503050 cites W2086680916 @default.
- W1999503050 cites W2093208620 @default.
- W1999503050 cites W2098105439 @default.
- W1999503050 cites W2100038753 @default.
- W1999503050 cites W2100763819 @default.
- W1999503050 cites W2113439647 @default.
- W1999503050 cites W2114170740 @default.
- W1999503050 cites W2114538487 @default.
- W1999503050 cites W2122081858 @default.
- W1999503050 cites W2123022008 @default.
- W1999503050 cites W2125474652 @default.
- W1999503050 cites W2126107464 @default.
- W1999503050 cites W2133787976 @default.
- W1999503050 cites W2139339609 @default.
- W1999503050 cites W2144022436 @default.
- W1999503050 cites W2144169031 @default.
- W1999503050 cites W2154236875 @default.
- W1999503050 cites W2158814411 @default.
- W1999503050 cites W2159330083 @default.
- W1999503050 cites W2162810346 @default.
- W1999503050 cites W2163594803 @default.
- W1999503050 cites W2163752312 @default.
- W1999503050 cites W2164160141 @default.
- W1999503050 cites W2166079009 @default.
- W1999503050 cites W2168115520 @default.
- W1999503050 cites W2169334356 @default.
- W1999503050 cites W2314920404 @default.
- W1999503050 cites W2323834210 @default.
- W1999503050 doi "https://doi.org/10.1016/j.ydbio.2012.02.010" @default.