FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999511752> ?p ?o ?g. }

• W1999511752 endingPage "752" @default.
• W1999511752 startingPage "744" @default.
• W1999511752 abstract "The elegant architecture of the channel of bacteriophage phi29 DNA packaging motor has inspired the development of biomimetics for biophysical and nanobiomedical applications. The reengineered channel inserted into a lipid membrane exhibits robust electrophysiological properties ideal for precise sensing and fingerprinting of dsDNA at the single-molecule level. Herein, we used single channel conduction assays to quantitatively evaluate the translocation dynamics of dsDNA as a function of the length and conformation of dsDNA. We extracted the speed of dsDNA translocation from the dwell time distribution and estimated the various forces involved in the translocation process. A ∼35-fold slower speed of translocation per base-pair was observed for long dsDNA, a significant contrast to the speed of dsDNA crossing synthetic pores. It was found that the channel could translocate both dsDNA with ∼32% of channel current blockage and with ∼64% for tetra-stranded DNA (two parallel dsDNA). The calculation of both cross-sectional areas of the dsDNA and tetra-stranded DNA suggested that the blockage was purely proportional to the physical space of the channel lumen and the size of the DNA substrate. Folded dsDNA configuration was clearly reflected in their characteristic current signatures. The finding of translocation of tetra-stranded DNA with 64% blockage is in consent with the recently elucidated mechanism of viral DNA packaging via a revolution mode that requires a channel larger than the dsDNA diameter of 2 nm to provide room for viral DNA revolving without rotation. The understanding of the dynamics of dsDNA translocation in the phi29 system will enable us to design more sophisticated single pore DNA translocation devices for future applications in nanotechnology and personal medicine." @default.
• W1999511752 created "2016-06-24" @default.
• W1999511752 creator A5015978857 @default.
• W1999511752 creator A5034435741 @default.
• W1999511752 creator A5037989560 @default.
• W1999511752 creator A5039967247 @default.
• W1999511752 creator A5069765017 @default.
• W1999511752 creator A5091493369 @default.
• W1999511752 date "2015-06-01" @default.
• W1999511752 modified "2023-10-16" @default.
• W1999511752 title "Single pore translocation of folded, double-stranded, and tetra-stranded DNA through channel of bacteriophage phi29 DNA packaging motor" @default.
• W1999511752 cites W1964495926 @default.
• W1999511752 cites W1965251627 @default.
• W1999511752 cites W1966384509 @default.
• W1999511752 cites W1966392758 @default.
• W1999511752 cites W1968217555 @default.
• W1999511752 cites W1971212710 @default.
• W1999511752 cites W1981693389 @default.
• W1999511752 cites W1984071764 @default.
• W1999511752 cites W1989398152 @default.
• W1999511752 cites W1989918649 @default.
• W1999511752 cites W1990652337 @default.
• W1999511752 cites W1992539915 @default.
• W1999511752 cites W1995472136 @default.
• W1999511752 cites W1997647778 @default.
• W1999511752 cites W2014550827 @default.
• W1999511752 cites W2014957444 @default.
• W1999511752 cites W2015876589 @default.
• W1999511752 cites W2024166174 @default.
• W1999511752 cites W2025583382 @default.
• W1999511752 cites W2027009369 @default.
• W1999511752 cites W2027593760 @default.
• W1999511752 cites W2028120697 @default.
• W1999511752 cites W2042894082 @default.
• W1999511752 cites W2044895688 @default.
• W1999511752 cites W2047188754 @default.
• W1999511752 cites W2047224017 @default.
• W1999511752 cites W2048464086 @default.
• W1999511752 cites W2053457342 @default.
• W1999511752 cites W2054003515 @default.
• W1999511752 cites W2059231905 @default.
• W1999511752 cites W2059265460 @default.
• W1999511752 cites W2060323119 @default.
• W1999511752 cites W2063702812 @default.
• W1999511752 cites W2063962302 @default.
• W1999511752 cites W2066555851 @default.
• W1999511752 cites W2075646926 @default.
• W1999511752 cites W2088740266 @default.
• W1999511752 cites W2090924278 @default.
• W1999511752 cites W2095030462 @default.
• W1999511752 cites W2097527643 @default.
• W1999511752 cites W2097973495 @default.
• W1999511752 cites W2110584587 @default.
• W1999511752 cites W2111440720 @default.
• W1999511752 cites W2113231874 @default.
• W1999511752 cites W2113817355 @default.
• W1999511752 cites W2126023294 @default.
• W1999511752 cites W2127332054 @default.
• W1999511752 cites W2127407003 @default.
• W1999511752 cites W2129234254 @default.
• W1999511752 cites W2129979528 @default.
• W1999511752 cites W2132659102 @default.
• W1999511752 cites W2137620025 @default.
• W1999511752 cites W2139446350 @default.
• W1999511752 cites W2142220869 @default.
• W1999511752 cites W2142929893 @default.
• W1999511752 cites W2142962840 @default.
• W1999511752 cites W2143070723 @default.
• W1999511752 cites W2143789454 @default.
• W1999511752 cites W2148332776 @default.
• W1999511752 cites W2151307366 @default.
• W1999511752 cites W2155096669 @default.
• W1999511752 cites W2156629890 @default.
• W1999511752 cites W2160947742 @default.
• W1999511752 cites W2162077400 @default.
• W1999511752 cites W2167381746 @default.
• W1999511752 cites W2312237980 @default.
• W1999511752 cites W2315037307 @default.
• W1999511752 cites W2328788097 @default.
• W1999511752 cites W283021492 @default.
• W1999511752 cites W3030856093 @default.
• W1999511752 doi "https://doi.org/10.1016/j.biomaterials.2015.02.104" @default.
• W1999511752 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4405662" @default.
• W1999511752 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25890769" @default.
• W1999511752 hasPublicationYear "2015" @default.
• W1999511752 type Work @default.
• W1999511752 sameAs 1999511752 @default.
• W1999511752 citedByCount "32" @default.
• W1999511752 countsByYear W19995117522015 @default.
• W1999511752 countsByYear W19995117522016 @default.
• W1999511752 countsByYear W19995117522017 @default.
• W1999511752 countsByYear W19995117522018 @default.
• W1999511752 countsByYear W19995117522019 @default.
• W1999511752 countsByYear W19995117522020 @default.
• W1999511752 countsByYear W19995117522021 @default.
• W1999511752 countsByYear W19995117522022 @default.
• W1999511752 countsByYear W19995117522023 @default.
• W1999511752 crossrefType "journal-article" @default.

• next