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- W1999528713 abstract "1. The application of a commercial fluorometer for the measurement of pyridine nucleotide reduction and oxidation of biological particulate preparations, such as mitochondria, yeast, and bacteria, has been described and demonstrated. 2. The changes of concentration of reduced pyridine nucleotide as measured fluorometrically with rat liver mitochondria have been compared to the spectrophotometric measurement of pyridine nucleotide kinetics and to the changes in pyridine nucleotide concentration determined by chemical analyses. 3. These studies show a fluorescence enhancement for reduced pyridine nucleotide of rat liver mitochondria of 4- to 6-fold suggesting the presence of a ternary complexing of pyridine nucleotide in mitochondria. The greatest fluorescence enhancement is observed with the DPNH fraction of mitochondria with increases as high as 8 to 12 obtainable under various metabolic conditions. 4. The extent of fluorescence enhancement observed with mitochondria, bakers' yeast, and E. coli is compared to previous reports on the DPNH complexing with purified enzyme preparations. 5. The influences of mitochondrial concentration and temperature on the measurement of mitochondrial concentration and temperature on the measurement of pyridine nucleotide fluorescence are described." @default.
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- W1999528713 date "1962-09-01" @default.
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- W1999528713 title "Fluorometric measurement of reduced pyridine nucleotide in cellular and subcellular particles" @default.
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- W1999528713 doi "https://doi.org/10.1016/0003-2697(62)90006-4" @default.
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