FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999537037> ?p ?o ?g. }

• W1999537037 endingPage "11621" @default.
• W1999537037 startingPage "11612" @default.
• W1999537037 abstract "Ribonucleotide reductases (RNRs) catalyze the conversion of nucleoside 5′-diphosphates to the corresponding deoxynucleotides supplying the dNTPs required for DNA replication and DNA repair. Class I RNRs require two subunits, α and β, for activity. Humans possess two β subunits: one involved in S phase DNA replication (β) and a second in mitochondrial DNA replication (β′ or p53R2) and potentially DNA repair. Gemcitabine (F2C) is used clinically as an anticancer agent, and its phosphorylated metabolites target many enzymes involved in nucleotide metabolism, including RNR. The present investigation with α (specific activity of 400 nmol min−1 mg−1) and β′ (0.6 Y·/β′2 and a specific activity of 420 nmol min−1 mg−1) establishes that F2CDP is a substoichiometric inactivator of RNR. Incubation of this α/β′ with [1′-3H]-F2CDP or [5-3H]-F2CDP and reisolation of the protein by Sephadex G-50 chromatography resulted in recovery 0.5 equiv of covalently bound sugar and 0.03 equiv of tightly associated cytosine to α2. SDS−PAGE analysis (loaded without boiling) of the inactivated RNR showed that 60% of α migrates as a 90 kDa protein and 40% as a 120 kDa protein. Incubation of [1′-3H]-F2CDP with active site mutants C444S/A, C218S/A, and E431Q/D-α and the C-terminal tail C787S/A and C790S/A mutants reveals that no sugar label is bound to the active site mutants of α and that, in the case of C218S-α, α migrates as a 90 kDa protein. Analysis of the inactivated wt-α/β′ RNR by size exclusion chromatography indicates a quaternary structure of α6β′6. A mechanism of inactivation common with hα/β is presented." @default.
• W1999537037 created "2016-06-24" @default.
• W1999537037 creator A5004286593 @default.
• W1999537037 creator A5024009979 @default.
• W1999537037 creator A5042241049 @default.
• W1999537037 date "2009-11-18" @default.
• W1999537037 modified "2023-09-27" @default.
• W1999537037 title "Mechanism of Inactivation of Human Ribonucleotide Reductase with p53R2 by Gemcitabine 5′-Diphosphate" @default.
• W1999537037 cites W1493988001 @default.
• W1999537037 cites W1574171361 @default.
• W1999537037 cites W163293632 @default.
• W1999537037 cites W1640573482 @default.
• W1999537037 cites W1680634601 @default.
• W1999537037 cites W1889637455 @default.
• W1999537037 cites W1967098277 @default.
• W1999537037 cites W1968118415 @default.
• W1999537037 cites W1969617308 @default.
• W1999537037 cites W1971843413 @default.
• W1999537037 cites W1973159565 @default.
• W1999537037 cites W1973844844 @default.
• W1999537037 cites W1974091141 @default.
• W1999537037 cites W1983901754 @default.
• W1999537037 cites W1993491727 @default.
• W1999537037 cites W2005278252 @default.
• W1999537037 cites W2009486080 @default.
• W1999537037 cites W2017940275 @default.
• W1999537037 cites W2021817174 @default.
• W1999537037 cites W2027478967 @default.
• W1999537037 cites W2028225701 @default.
• W1999537037 cites W2028371684 @default.
• W1999537037 cites W2032839609 @default.
• W1999537037 cites W2037469012 @default.
• W1999537037 cites W2037851792 @default.
• W1999537037 cites W2046237091 @default.
• W1999537037 cites W2047348882 @default.
• W1999537037 cites W2059013576 @default.
• W1999537037 cites W2060688328 @default.
• W1999537037 cites W2068456988 @default.
• W1999537037 cites W2068973845 @default.
• W1999537037 cites W2074775358 @default.
• W1999537037 cites W2082820112 @default.
• W1999537037 cites W2086390600 @default.
• W1999537037 cites W2088666151 @default.
• W1999537037 cites W2090684905 @default.
• W1999537037 cites W2095160934 @default.
• W1999537037 cites W2095287544 @default.
• W1999537037 cites W2107584699 @default.
• W1999537037 cites W2117278104 @default.
• W1999537037 cites W2153735696 @default.
• W1999537037 cites W2154959891 @default.
• W1999537037 cites W2322201734 @default.
• W1999537037 cites W2415937145 @default.
• W1999537037 cites W4256321774 @default.
• W1999537037 doi "https://doi.org/10.1021/bi901588z" @default.
• W1999537037 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2917093" @default.
• W1999537037 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19899807" @default.
• W1999537037 hasPublicationYear "2009" @default.
• W1999537037 type Work @default.
• W1999537037 sameAs 1999537037 @default.
• W1999537037 citedByCount "48" @default.
• W1999537037 countsByYear W19995370372012 @default.
• W1999537037 countsByYear W19995370372013 @default.
• W1999537037 countsByYear W19995370372014 @default.
• W1999537037 countsByYear W19995370372015 @default.
• W1999537037 countsByYear W19995370372016 @default.
• W1999537037 countsByYear W19995370372017 @default.
• W1999537037 countsByYear W19995370372018 @default.
• W1999537037 countsByYear W19995370372019 @default.
• W1999537037 countsByYear W19995370372020 @default.
• W1999537037 countsByYear W19995370372021 @default.
• W1999537037 countsByYear W19995370372022 @default.
• W1999537037 countsByYear W19995370372023 @default.
• W1999537037 crossrefType "journal-article" @default.
• W1999537037 hasAuthorship W1999537037A5004286593 @default.
• W1999537037 hasAuthorship W1999537037A5024009979 @default.
• W1999537037 hasAuthorship W1999537037A5042241049 @default.
• W1999537037 hasBestOaLocation W19995370372 @default.
• W1999537037 hasConcept C104292427 @default.
• W1999537037 hasConcept C104317684 @default.
• W1999537037 hasConcept C153911025 @default.
• W1999537037 hasConcept C181199279 @default.
• W1999537037 hasConcept C185592680 @default.
• W1999537037 hasConcept C2776543447 @default.
• W1999537037 hasConcept C2780535078 @default.
• W1999537037 hasConcept C4710235 @default.
• W1999537037 hasConcept C512185932 @default.
• W1999537037 hasConcept C552990157 @default.
• W1999537037 hasConcept C55493867 @default.
• W1999537037 hasConcept C73573662 @default.
• W1999537037 hasConcept C86803240 @default.
• W1999537037 hasConcept C98892162 @default.
• W1999537037 hasConceptScore W1999537037C104292427 @default.
• W1999537037 hasConceptScore W1999537037C104317684 @default.
• W1999537037 hasConceptScore W1999537037C153911025 @default.
• W1999537037 hasConceptScore W1999537037C181199279 @default.
• W1999537037 hasConceptScore W1999537037C185592680 @default.
• W1999537037 hasConceptScore W1999537037C2776543447 @default.
• W1999537037 hasConceptScore W1999537037C2780535078 @default.

• next