Matches in SemOpenAlex for { <https://semopenalex.org/work/W199958257> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W199958257 endingPage "75" @default.
- W199958257 startingPage "64" @default.
- W199958257 abstract "In the current study two monoclonal antibodies (mAb) were used to investigate the expression of adult acinar and duct cell-specific antigens and their relationship with cell growth in primary acinar cell cultures. We have previously found that adult mouse pancreatic acinar cells divide in primary culture. Furthermore, during growth the cells lose their differentiated morphology and exhibit decreased expression of secretory proteins, followed by some degree of morphological redifferentiation after reaching confluency. A mAb specific in the adult pancreas for acinar cells (mAb Acinar-1) and another specific in the adult pancreas for duct cells (mAb Duct-1) were generated using such cultures as the immunogen. The starting material for the cultures consisted of predominantly Acinar-1 positive cells which incorporated [3H]thymidine, as determined by autoradiography and immunofluorescence labeling. However, expression of the acinar antigen persisted for only the first 3 to 7 days in culture. By contrast, expression of the duct antigen was rare until after 5 days in culture and was highest at day 9, the peak of cell growth. Dual label immunofluorescence showed that during the growth phase fewer cells expressed the acinar antigen, most expressed the duct antigen, and occasional cells expressed both antigens. After reaching confluency, the growth rate declined from days 15 to 21, and the cells progressively regained the acinar antigen with a concomitant loss of the duct antigen. mAb labeling was morphometrically quantitated and showed that more than 97% of the labeled area was Acinar-1 positive at 3 days, which decreased to approximately 16% at day 9, and then returned to over 97% by day 21 of culture. Ultrastructural immunolabeling showed that Acinar-1 positive cells at 21 days had well organized rough endoplasmic reticulum and small apical vesicles, while Duct-1 positive cells were undifferentiated in appearance (day 9) or had numerous mitochondria (day 21). Thus, changes in cell-specific antigens were paralleled by cell type associated morphological characteristics and indicate that adult acinar cells can retrodifferentiate to a more duct-like cell while retaining the potential to express an acinar-specific antigen." @default.
- W199958257 created "2016-06-24" @default.
- W199958257 creator A5028009560 @default.
- W199958257 creator A5038163774 @default.
- W199958257 date "1990-02-01" @default.
- W199958257 modified "2023-09-23" @default.
- W199958257 title "Pancreatic acinar cells in culture: expression of acinar and ductal antigens in a growth-related manner." @default.
- W199958257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2184038" @default.
- W199958257 hasPublicationYear "1990" @default.
- W199958257 type Work @default.
- W199958257 sameAs 199958257 @default.
- W199958257 citedByCount "42" @default.
- W199958257 countsByYear W1999582572013 @default.
- W199958257 countsByYear W1999582572014 @default.
- W199958257 countsByYear W1999582572015 @default.
- W199958257 countsByYear W1999582572016 @default.
- W199958257 countsByYear W1999582572018 @default.
- W199958257 countsByYear W1999582572020 @default.
- W199958257 countsByYear W1999582572022 @default.
- W199958257 countsByYear W1999582572023 @default.
- W199958257 crossrefType "journal-article" @default.
- W199958257 hasAuthorship W199958257A5028009560 @default.
- W199958257 hasAuthorship W199958257A5038163774 @default.
- W199958257 hasConcept C134018914 @default.
- W199958257 hasConcept C147483822 @default.
- W199958257 hasConcept C153911025 @default.
- W199958257 hasConcept C159654299 @default.
- W199958257 hasConcept C203014093 @default.
- W199958257 hasConcept C2778236600 @default.
- W199958257 hasConcept C2778764654 @default.
- W199958257 hasConcept C2779960720 @default.
- W199958257 hasConcept C2780446580 @default.
- W199958257 hasConcept C2991930265 @default.
- W199958257 hasConcept C542903549 @default.
- W199958257 hasConcept C54355233 @default.
- W199958257 hasConcept C55493867 @default.
- W199958257 hasConcept C62112901 @default.
- W199958257 hasConcept C81885089 @default.
- W199958257 hasConcept C86803240 @default.
- W199958257 hasConceptScore W199958257C134018914 @default.
- W199958257 hasConceptScore W199958257C147483822 @default.
- W199958257 hasConceptScore W199958257C153911025 @default.
- W199958257 hasConceptScore W199958257C159654299 @default.
- W199958257 hasConceptScore W199958257C203014093 @default.
- W199958257 hasConceptScore W199958257C2778236600 @default.
- W199958257 hasConceptScore W199958257C2778764654 @default.
- W199958257 hasConceptScore W199958257C2779960720 @default.
- W199958257 hasConceptScore W199958257C2780446580 @default.
- W199958257 hasConceptScore W199958257C2991930265 @default.
- W199958257 hasConceptScore W199958257C542903549 @default.
- W199958257 hasConceptScore W199958257C54355233 @default.
- W199958257 hasConceptScore W199958257C55493867 @default.
- W199958257 hasConceptScore W199958257C62112901 @default.
- W199958257 hasConceptScore W199958257C81885089 @default.
- W199958257 hasConceptScore W199958257C86803240 @default.
- W199958257 hasIssue "1" @default.
- W199958257 hasLocation W1999582571 @default.
- W199958257 hasOpenAccess W199958257 @default.
- W199958257 hasPrimaryLocation W1999582571 @default.
- W199958257 hasRelatedWork W1598023268 @default.
- W199958257 hasRelatedWork W1662318117 @default.
- W199958257 hasRelatedWork W1894300370 @default.
- W199958257 hasRelatedWork W1989289676 @default.
- W199958257 hasRelatedWork W2037509311 @default.
- W199958257 hasRelatedWork W2073262237 @default.
- W199958257 hasRelatedWork W2109259166 @default.
- W199958257 hasRelatedWork W2148455206 @default.
- W199958257 hasRelatedWork W2317962315 @default.
- W199958257 hasRelatedWork W2581431957 @default.
- W199958257 hasVolume "51" @default.
- W199958257 isParatext "false" @default.
- W199958257 isRetracted "false" @default.
- W199958257 magId "199958257" @default.
- W199958257 workType "article" @default.