FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999596908> ?p ?o ?g. }

• W1999596908 endingPage "504" @default.
• W1999596908 startingPage "499" @default.
• W1999596908 abstract "Our previous in vivo study demonstrated that human nucleus pulposus chondrocytes (NPCs) in aging discs exhibited characteristic senescent features such as an increased senescence-associated β-galactosidase (SA-β-gal) expression, shortened telomere, and decreased telomerase activity. The replicative p53-p21-pRB pathway, rather than the stress-induced p16-pRB pathway, played a more important role in the senescence of NPCs in an in vivo condition, although there is a situation in which both the pathways can be activated simultaneously. However, the in vitro lifespan and senescence mechanisms of human NPCs remain unclear.To evaluate the underlying mechanisms of in vitro lifespan and in vitro senescence of the human NPCs and to verify whether the in vitro senescence mechanisms of the human NPC can represent the in vivo aging mechanisms of the cell.An in vitro study.We serially cultivated human NPCs from patients of different ages (35, 42, 55, 66, and 76 years) until the cells reached the end of their in vitro lifespan. During each subcultivation, we calculated NPCs cumulative population doubling level (PDL) and examined senescence markers (SA-β-gal, telomere length, telomerase activity, and p53, p21, pRB, and p16 expressions).The cumulative PDLs of the NPCs from patients aged 35, 42 55, 66, and 76 years were 32, 29, 11, 9, and 11, respectively. The younger patients (35 and 42 years) had a higher mean of cumulative PDLs than the elderly patients did (55, 66, and 76 years; 30.5 vs. 10.3; p=.001). In addition, there was a significant, negative correlation between the cumulative PDLs and patient's age (r=-0.89; p=.04). With advancing culture passages, the NPCs irrespective of patient's age exhibited characteristic senescent features, such as an increase in SA-β-gal expression, shortening of telomeres, decrease in telomerase activity, and activation of both replicative p53-p21-pRB and stress-induced p16-pRB pathways. However, all the senescent features occurred at the earlier passages in elderly compared with younger patients.The present study demonstrated that the human NPCs had a finite in vitro lifespan, which declined with host aging. The in vitro lifespan was determined by both replicative and stress-induced senescence mechanisms. The similarity in the in vitro senescent features with those apparent in the previous in vivo study suggests a possibility of the in vitro senescence mechanisms of the human NPC as a model of the in vivo aging mechanisms of the cell for future studies." @default.
• W1999596908 created "2016-06-24" @default.
• W1999596908 creator A5003391316 @default.
• W1999596908 creator A5022032476 @default.
• W1999596908 creator A5063480657 @default.
• W1999596908 date "2014-03-01" @default.
• W1999596908 modified "2023-10-14" @default.
• W1999596908 title "In vitro lifespan and senescence mechanisms of human nucleus pulposus chondrocytes" @default.
• W1999596908 cites W1848078943 @default.
• W1999596908 cites W1966188170 @default.
• W1999596908 cites W1998663807 @default.
• W1999596908 cites W2009569724 @default.
• W1999596908 cites W2011482325 @default.
• W1999596908 cites W2011779395 @default.
• W1999596908 cites W2021412978 @default.
• W1999596908 cites W2022553267 @default.
• W1999596908 cites W2024004426 @default.
• W1999596908 cites W2024209230 @default.
• W1999596908 cites W2026856852 @default.
• W1999596908 cites W2047330128 @default.
• W1999596908 cites W2051146905 @default.
• W1999596908 cites W2061619522 @default.
• W1999596908 cites W2062395030 @default.
• W1999596908 cites W2086095857 @default.
• W1999596908 cites W2095370504 @default.
• W1999596908 cites W2104908670 @default.
• W1999596908 cites W2110934787 @default.
• W1999596908 cites W2117912210 @default.
• W1999596908 cites W2139585666 @default.
• W1999596908 cites W4239524546 @default.
• W1999596908 doi "https://doi.org/10.1016/j.spinee.2013.06.099" @default.
• W1999596908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24345469" @default.
• W1999596908 hasPublicationYear "2014" @default.
• W1999596908 type Work @default.
• W1999596908 sameAs 1999596908 @default.
• W1999596908 citedByCount "46" @default.
• W1999596908 countsByYear W19995969082015 @default.
• W1999596908 countsByYear W19995969082016 @default.
• W1999596908 countsByYear W19995969082017 @default.
• W1999596908 countsByYear W19995969082018 @default.
• W1999596908 countsByYear W19995969082019 @default.
• W1999596908 countsByYear W19995969082020 @default.
• W1999596908 countsByYear W19995969082021 @default.
• W1999596908 countsByYear W19995969082022 @default.
• W1999596908 countsByYear W19995969082023 @default.
• W1999596908 crossrefType "journal-article" @default.
• W1999596908 hasAuthorship W1999596908A5003391316 @default.
• W1999596908 hasAuthorship W1999596908A5022032476 @default.
• W1999596908 hasAuthorship W1999596908A5063480657 @default.
• W1999596908 hasConcept C104317684 @default.
• W1999596908 hasConcept C16685009 @default.
• W1999596908 hasConcept C177336024 @default.
• W1999596908 hasConcept C202751555 @default.
• W1999596908 hasConcept C207001950 @default.
• W1999596908 hasConcept C2908647359 @default.
• W1999596908 hasConcept C522857546 @default.
• W1999596908 hasConcept C54355233 @default.
• W1999596908 hasConcept C552990157 @default.
• W1999596908 hasConcept C71924100 @default.
• W1999596908 hasConcept C86803240 @default.
• W1999596908 hasConcept C94581717 @default.
• W1999596908 hasConcept C95444343 @default.
• W1999596908 hasConcept C99454951 @default.
• W1999596908 hasConceptScore W1999596908C104317684 @default.
• W1999596908 hasConceptScore W1999596908C16685009 @default.
• W1999596908 hasConceptScore W1999596908C177336024 @default.
• W1999596908 hasConceptScore W1999596908C202751555 @default.
• W1999596908 hasConceptScore W1999596908C207001950 @default.
• W1999596908 hasConceptScore W1999596908C2908647359 @default.
• W1999596908 hasConceptScore W1999596908C522857546 @default.
• W1999596908 hasConceptScore W1999596908C54355233 @default.
• W1999596908 hasConceptScore W1999596908C552990157 @default.
• W1999596908 hasConceptScore W1999596908C71924100 @default.
• W1999596908 hasConceptScore W1999596908C86803240 @default.
• W1999596908 hasConceptScore W1999596908C94581717 @default.
• W1999596908 hasConceptScore W1999596908C95444343 @default.
• W1999596908 hasConceptScore W1999596908C99454951 @default.
• W1999596908 hasIssue "3" @default.
• W1999596908 hasLocation W19995969081 @default.
• W1999596908 hasLocation W19995969082 @default.
• W1999596908 hasOpenAccess W1999596908 @default.
• W1999596908 hasPrimaryLocation W19995969081 @default.
• W1999596908 hasRelatedWork W2005903392 @default.
• W1999596908 hasRelatedWork W2050296416 @default.
• W1999596908 hasRelatedWork W2090447055 @default.
• W1999596908 hasRelatedWork W2103156000 @default.
• W1999596908 hasRelatedWork W2105231446 @default.
• W1999596908 hasRelatedWork W2127443475 @default.
• W1999596908 hasRelatedWork W2371502216 @default.
• W1999596908 hasRelatedWork W2400859286 @default.
• W1999596908 hasRelatedWork W2417511905 @default.
• W1999596908 hasRelatedWork W60632522 @default.
• W1999596908 hasVolume "14" @default.
• W1999596908 isParatext "false" @default.
• W1999596908 isRetracted "false" @default.
• W1999596908 magId "1999596908" @default.
• W1999596908 workType "article" @default.