FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999711679> ?p ?o ?g. }

• W1999711679 endingPage "265" @default.
• W1999711679 startingPage "250" @default.
• W1999711679 abstract "Intracerebral administration of kainic acid (KA) in rats was previously shown to abolish immunohistochemical labelling for the astrocytic gap junction protein connexin43 (Cx43) at sites depleted of neurons (Vukelic et al: Neurosci Lett 130:120–124, 1991). This response of Cx43 has now been further investigated with a number of different sequence-specific anti-Cx43 antibodies. At lesion sites in the thalamus, striatum, and hippocampus examined immunohistochemically with an antibody against amino acids (aa's) 346–363 in the Cx43 sequence, the antibody used in the earlier study, Cx43-immunoreactivity was increased 5 h after KA injections, absent by 24 h and for up to 2 weeks post-injection, and began to return to less than normal levels by 2 to 3 weeks post-injection. Analyses of KA lesion sites with antibodies against other sequences of Cx43 (amino acids 283–298, 253–270, 241–260, 113–123, and 49–61) revealed not only the presence but in some cases an increased density of Cx43 immunoreactivity after a survival time of 1 week. Immunolabelling patterns at these sites consisted of relatively large, coarse profiles rather the fine punctate labelling typically seen in sections of normal brain. In homogenates of KA-injected striatum analyzed by Western blots, Cx43 was detected at near normal or slightly increased levels at various survival times examined. The 43 kDa phosphorylated form of Cx43 and its faster migrating 41 kDa dephosphorylated form which is generated post-mortem by a brain phosphatase were both present after standard methods of tissue preparation for Western blot analysis, while only the 43 kDa form was present in normal and KA-injected striatum after inactivation of brain metabolism by focused cranial microwave irradiation. Ultrastructural investigations of lesions sites within the thalamus revealed a virtual absence of astrocytic gap junctions. These results demonstrate that Cx43 levels initially increase after intracerebral KA treatment, that its molecular organization in resident astrocytes is altered such that epitopes that are normally accessible to antibody are hidden while those that may be hidden or relatively inaccessible are exposed, and that this molecular alteration in Cx43 is associated with loss of astrocytic gap junctions. © 1994 Wiley-Liss, Inc." @default.
• W1999711679 created "2016-06-24" @default.
• W1999711679 creator A5014997892 @default.
• W1999711679 creator A5018313164 @default.
• W1999711679 creator A5061726638 @default.
• W1999711679 creator A5068819082 @default.
• W1999711679 creator A5080359727 @default.
• W1999711679 date "1994-04-01" @default.
• W1999711679 modified "2023-09-25" @default.
• W1999711679 title "Kainic acid induced alterations in antibody recognition of connexin43 and loss of astrocytic gap junctions in rat brain" @default.
• W1999711679 cites W1550229015 @default.
• W1999711679 cites W1563929437 @default.
• W1999711679 cites W1934976538 @default.
• W1999711679 cites W1975169487 @default.
• W1999711679 cites W1981595069 @default.
• W1999711679 cites W1981744489 @default.
• W1999711679 cites W1983460238 @default.
• W1999711679 cites W1983983350 @default.
• W1999711679 cites W1986249764 @default.
• W1999711679 cites W1988316236 @default.
• W1999711679 cites W1995678825 @default.
• W1999711679 cites W2000447621 @default.
• W1999711679 cites W2001320373 @default.
• W1999711679 cites W2003615812 @default.
• W1999711679 cites W2004759336 @default.
• W1999711679 cites W2006289803 @default.
• W1999711679 cites W2006504264 @default.
• W1999711679 cites W2011387965 @default.
• W1999711679 cites W2024773604 @default.
• W1999711679 cites W2033215167 @default.
• W1999711679 cites W2033924125 @default.
• W1999711679 cites W2037458621 @default.
• W1999711679 cites W2040821726 @default.
• W1999711679 cites W2043668221 @default.
• W1999711679 cites W2045686292 @default.
• W1999711679 cites W2055745158 @default.
• W1999711679 cites W2056482899 @default.
• W1999711679 cites W2058260122 @default.
• W1999711679 cites W2058358053 @default.
• W1999711679 cites W2064488060 @default.
• W1999711679 cites W2069193323 @default.
• W1999711679 cites W2071307343 @default.
• W1999711679 cites W2074419686 @default.
• W1999711679 cites W2076835453 @default.
• W1999711679 cites W2077660651 @default.
• W1999711679 cites W2085310064 @default.
• W1999711679 cites W2086149913 @default.
• W1999711679 cites W2086711694 @default.
• W1999711679 cites W2087587497 @default.
• W1999711679 cites W2092384816 @default.
• W1999711679 cites W2095272418 @default.
• W1999711679 cites W2099684738 @default.
• W1999711679 cites W2116763991 @default.
• W1999711679 cites W2123929920 @default.
• W1999711679 cites W2137820120 @default.
• W1999711679 cites W2188385533 @default.
• W1999711679 cites W3004013953 @default.
• W1999711679 cites W4236886230 @default.
• W1999711679 doi "https://doi.org/10.1002/glia.440100404" @default.
• W1999711679 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8056437" @default.
• W1999711679 hasPublicationYear "1994" @default.
• W1999711679 type Work @default.
• W1999711679 sameAs 1999711679 @default.
• W1999711679 citedByCount "55" @default.
• W1999711679 countsByYear W19997116792012 @default.
• W1999711679 countsByYear W19997116792013 @default.
• W1999711679 countsByYear W19997116792017 @default.
• W1999711679 countsByYear W19997116792019 @default.
• W1999711679 countsByYear W19997116792020 @default.
• W1999711679 crossrefType "journal-article" @default.
• W1999711679 hasAuthorship W1999711679A5014997892 @default.
• W1999711679 hasAuthorship W1999711679A5018313164 @default.
• W1999711679 hasAuthorship W1999711679A5061726638 @default.
• W1999711679 hasAuthorship W1999711679A5068819082 @default.
• W1999711679 hasAuthorship W1999711679A5080359727 @default.
• W1999711679 hasConcept C104317684 @default.
• W1999711679 hasConcept C126322002 @default.
• W1999711679 hasConcept C134018914 @default.
• W1999711679 hasConcept C142724271 @default.
• W1999711679 hasConcept C153911025 @default.
• W1999711679 hasConcept C158157758 @default.
• W1999711679 hasConcept C159654299 @default.
• W1999711679 hasConcept C169760540 @default.
• W1999711679 hasConcept C170493617 @default.
• W1999711679 hasConcept C203014093 @default.
• W1999711679 hasConcept C204232928 @default.
• W1999711679 hasConcept C2776108384 @default.
• W1999711679 hasConcept C2776415932 @default.
• W1999711679 hasConcept C2779246727 @default.
• W1999711679 hasConcept C2780062018 @default.
• W1999711679 hasConcept C2781156865 @default.
• W1999711679 hasConcept C2781161787 @default.
• W1999711679 hasConcept C513476851 @default.
• W1999711679 hasConcept C55493867 @default.
• W1999711679 hasConcept C61174792 @default.
• W1999711679 hasConcept C71924100 @default.
• W1999711679 hasConcept C79879829 @default.
• W1999711679 hasConcept C85265743 @default.

• next