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- W1999736474 abstract "Abstract Three different citrus‐producing regions of Turkey—Edremit Gulf, Coastal Aegean, and eastern Black Sea—were surveyed in 2005 and 2006. A total of 119 samples were collected from Satsuma Owari mandarin (Citrus unshiu) trees grafted on Citrus tristeza virus (CTV)‐resistant Poncirus trifoliata rootstocks in commercial groves and home gardens. All samples were tested for the presence of CTV by direct tissue blot immunoassay (DTBIA) and double antibody sandwich enzyme‐linked immunosorbent assay (DAS‐ELISA) using young shoots. The samples that tested positive were indexed for biological properties. The results obtained from DTBIA tests showed that 20 of 119 (16.8%) tested trees were infected with CTV and 99 trees were virus free. All samples that tested positive in DTBIA were also positive in DAS‐ELISA. After biological indexing, none of these 20 isolates showed any symptoms on sour orange, grapefruit, and sweet orange plants, but all isolates induced vein‐clearing symptoms on Mexican lime (Citrus aurantifoli) in 9 months. Some isolates also caused leaf‐cupping and chlorosis in Mexican lime. However, 15 months after initial grafting, all isolates induced mild to moderate stem‐pitting on Mexican lime. In addition, young tissues, petioles, and leaf samples were collected periodically at monthly intervals for 1 year (2006) from a 25‐year‐old Satsuma Owari mandarin grafted on P. trifoliata in the Edremit Gulf. CTV was readily detected in tissue blots of young shoots and petioles from CTV‐infected plants during the autumn, winter, and spring seasons. Similarly, the highest ELISA values were obtained in April; the lowest values were noted in September. This study showed that DTBIA is a rapid, sensitive, and reliable procedure for detecting CTV under field conditions." @default.
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- W1999736474 date "2008-12-01" @default.
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- W1999736474 title "Detection of<i>Citrus tristeza virus</i>(CTV) from Satsuma Owari mandarins<i>(Citris unshiu)</i>by direct tissue blot immunoassay (DTBIA), DAS‐ELISA, and biological indexing" @default.
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- W1999736474 doi "https://doi.org/10.1080/01140670809510240" @default.
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