FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999746543> ?p ?o ?g. }

• W1999746543 endingPage "e37263" @default.
• W1999746543 startingPage "e37263" @default.
• W1999746543 abstract "Background The hypervirulent Clostridium difficile ribotype 027 can be classified into subtypes, but it unknown if these differ in terms of severity of C. difficile infection (CDI). Genomic studies of C. difficile 027 strains have established that they are rich in mobile genetic elements including prophages. This study combined physiological studies, electron microscopy analysis and molecular biology to determine the potential role of temperate bacteriophages in disease and diversity of C. difficile 027. Methodology/Principal Findings We induced prophages from 91 clinical C. difficile 027 isolates and used transmission electron microscopy and pulsed-field gel electrophoresis to characterise the bacteriophages present. We established a correlation between phage morphology and subtype. Morphologically distinct tailed bacteriophages belonging to Myoviridae and Siphoviridae were identified in 63 and three isolates, respectively. Dual phage carriage was observed in four isolates. In addition, there were inducible phage tail-like particles (PT-LPs) in all isolates. The capacity of two antibiotics mitomycin C and norfloxacin to induce prophages was compared and it was shown that they induced specific prophages from C. difficile isolates. A PCR assay targeting the capsid gene of the myoviruses was designed to examine molecular diversity of C. difficile myoviruses. Phylogenetic analysis of the capsid gene sequences from eight ribotypes showed that all sequences found in the ribotype 027 isolates were identical and distinct from other C. difficile ribotypes and other bacteria species. Conclusion/Significance A diverse set of temperate bacteriophages are associated with C. difficile 027. The observed correlation between phage carriage and the subtypes suggests that temperate bacteriophages contribute to the diversity of C. difficile 027 and may play a role in severity of disease associated with this ribotype. The capsid gene can be used as a tool to identify C. difficile myoviruses present within bacterial genomes." @default.
• W1999746543 created "2016-06-24" @default.
• W1999746543 creator A5004027687 @default.
• W1999746543 creator A5027928189 @default.
• W1999746543 creator A5042042350 @default.
• W1999746543 creator A5050645245 @default.
• W1999746543 creator A5052002914 @default.
• W1999746543 creator A5059548108 @default.
• W1999746543 date "2012-05-18" @default.
• W1999746543 modified "2023-10-16" @default.
• W1999746543 title "Diverse Temperate Bacteriophage Carriage in Clostridium difficile 027 Strains" @default.
• W1999746543 cites W1016350759 @default.
• W1999746543 cites W1516888617 @default.
• W1999746543 cites W1570011717 @default.
• W1999746543 cites W1763981959 @default.
• W1999746543 cites W1977241950 @default.
• W1999746543 cites W1981824109 @default.
• W1999746543 cites W1983883872 @default.
• W1999746543 cites W2007890464 @default.
• W1999746543 cites W2012267788 @default.
• W1999746543 cites W2016106610 @default.
• W1999746543 cites W2018869205 @default.
• W1999746543 cites W2026254795 @default.
• W1999746543 cites W2026599933 @default.
• W1999746543 cites W2036615287 @default.
• W1999746543 cites W2050783903 @default.
• W1999746543 cites W2088213857 @default.
• W1999746543 cites W2093917326 @default.
• W1999746543 cites W2101902374 @default.
• W1999746543 cites W2109201412 @default.
• W1999746543 cites W2113721855 @default.
• W1999746543 cites W2117491708 @default.
• W1999746543 cites W2120051415 @default.
• W1999746543 cites W2125343055 @default.
• W1999746543 cites W2127088578 @default.
• W1999746543 cites W2127385500 @default.
• W1999746543 cites W2131434685 @default.
• W1999746543 cites W2141951202 @default.
• W1999746543 cites W2145207523 @default.
• W1999746543 cites W2149544927 @default.
• W1999746543 cites W2151351206 @default.
• W1999746543 cites W2157128604 @default.
• W1999746543 cites W2158524196 @default.
• W1999746543 cites W2170375814 @default.
• W1999746543 cites W2292857696 @default.
• W1999746543 cites W35416720 @default.
• W1999746543 doi "https://doi.org/10.1371/journal.pone.0037263" @default.
• W1999746543 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3356267" @default.
• W1999746543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22624004" @default.
• W1999746543 hasPublicationYear "2012" @default.
• W1999746543 type Work @default.
• W1999746543 sameAs 1999746543 @default.
• W1999746543 citedByCount "51" @default.
• W1999746543 countsByYear W19997465432012 @default.
• W1999746543 countsByYear W19997465432013 @default.
• W1999746543 countsByYear W19997465432014 @default.
• W1999746543 countsByYear W19997465432015 @default.
• W1999746543 countsByYear W19997465432016 @default.
• W1999746543 countsByYear W19997465432017 @default.
• W1999746543 countsByYear W19997465432018 @default.
• W1999746543 countsByYear W19997465432019 @default.
• W1999746543 countsByYear W19997465432020 @default.
• W1999746543 countsByYear W19997465432021 @default.
• W1999746543 countsByYear W19997465432022 @default.
• W1999746543 countsByYear W19997465432023 @default.
• W1999746543 crossrefType "journal-article" @default.
• W1999746543 hasAuthorship W1999746543A5004027687 @default.
• W1999746543 hasAuthorship W1999746543A5027928189 @default.
• W1999746543 hasAuthorship W1999746543A5042042350 @default.
• W1999746543 hasAuthorship W1999746543A5050645245 @default.
• W1999746543 hasAuthorship W1999746543A5052002914 @default.
• W1999746543 hasAuthorship W1999746543A5059548108 @default.
• W1999746543 hasBestOaLocation W19997465431 @default.
• W1999746543 hasConcept C104317684 @default.
• W1999746543 hasConcept C159047783 @default.
• W1999746543 hasConcept C197480550 @default.
• W1999746543 hasConcept C2776441376 @default.
• W1999746543 hasConcept C2778303719 @default.
• W1999746543 hasConcept C2780128593 @default.
• W1999746543 hasConcept C2780984408 @default.
• W1999746543 hasConcept C2994496256 @default.
• W1999746543 hasConcept C501593827 @default.
• W1999746543 hasConcept C54355233 @default.
• W1999746543 hasConcept C547475151 @default.
• W1999746543 hasConcept C73445445 @default.
• W1999746543 hasConcept C86803240 @default.
• W1999746543 hasConcept C89423630 @default.
• W1999746543 hasConceptScore W1999746543C104317684 @default.
• W1999746543 hasConceptScore W1999746543C159047783 @default.
• W1999746543 hasConceptScore W1999746543C197480550 @default.
• W1999746543 hasConceptScore W1999746543C2776441376 @default.
• W1999746543 hasConceptScore W1999746543C2778303719 @default.
• W1999746543 hasConceptScore W1999746543C2780128593 @default.
• W1999746543 hasConceptScore W1999746543C2780984408 @default.
• W1999746543 hasConceptScore W1999746543C2994496256 @default.
• W1999746543 hasConceptScore W1999746543C501593827 @default.
• W1999746543 hasConceptScore W1999746543C54355233 @default.
• W1999746543 hasConceptScore W1999746543C547475151 @default.

• next