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- W1999751574 abstract "A haemolytic plaque-forming cell (PFC) assay detecting human B lymphocytes secreting IgG rheumatoid factor (RF) was established using sheep erythrocytes (SRBC) sensitized with rabbit IgG, developing rabbit anti-human IgG, and complement. IgG–RF PFC were only demonstrated with IgG–depleted guinea-pig serum as the source of complement. Cells spontaneously secreting IgG-RF were found among synovial fluid mononuclear cells (mean, 134/106) and synovial tissue mononuclear cells (mean, 1775/106) from patients with rheumatoid arthritis, whereas few were recorded among blood lymphocytes (mean, 3/106). The experiments revealed that the RF–IgG PFC were protein-synthesizing B lymphocytes. The antibody specificity of the secreted IgF-RF was verified by the inhibitory effect of exogenous human and rabbit IgG on PFC formation." @default.
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- W1999751574 date "1983-05-01" @default.
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- W1999751574 title "IgG Rheumatoid Factor-Secreting Lymphocytes in Rheumatoid Arthritis: Evaluation of a Haemolytic Plaque-Forming Cell Technique" @default.
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- W1999751574 doi "https://doi.org/10.1111/j.1365-3083.1983.tb00814.x" @default.
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