FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999838991> ?p ?o ?g. }

• W1999838991 abstract "Our purpose was to obtain genome-wide expression data for the rabbit species on the responses of peripheral blood mononuclear cells (PBMCs) after in vitro stimulation by lipopolysaccharide (LPS) or phorbol myristate acetate (PMA) and ionomycin. This transcriptome profiling was carried out using microarrays enriched with immunity-related genes, and annotated with the most recent data available for the rabbit genome. The LPS affected 15 to 20 times fewer genes than PMA-Ionomycin after both 4 hours (T4) and 24 hours (T24), of in vitro stimulation, in comparison with mock-stimulated PBMCs. LPS induced an inflammatory response as shown by a significant up-regulation of IL12A and CXCL11 at T4, followed by an increased transcription of IL6, IL1B, IL1A, IL36, IL37, TNF, and CCL4 at T24. Surprisingly, we could not find an up-regulation of IL8 either at T4 or at T24, and detected a down-regulation of DEFB1 and BPI at T24. A concerted up-regulation of SAA1, S100A12 and F3 was found upon stimulation by LPS. PMA-Ionomycin induced a very early expression of Th1, Th2, Treg, and Th17 responses by PBMCs at T4. The Th1 response increased at T24 as shown by the increase of the transcription of IFNG and by contrast to other cytokines which significantly decreased from T4 to T24 (IL2, IL4, IL10, IL13, IL17A, CD69) by comparison to mock-stimulation. The granulocyte-macrophage colony-stimulating factor (CSF2) was by far the most over-expressed gene at both T4 and T24 by comparison to mock-stimulated cells, confirming a major impact of PMA-Ionomycin on cell growth and proliferation. A significant down-regulation of IL16 was observed at T4 and T24, in agreement with a role of IL16 in PBMC apoptosis. We report new data on the responses of PBMCs to LPS and PMA-Ionomycin in the rabbit species, thus enlarging the set of mammalian species for which such reports exist. The availability of the rabbit genome assembly together with high throughput genomic tools should pave the way for more intense genomic studies for this species, which is known to be a very relevant biomedical model in immunology and physiology." @default.
• W1999838991 created "2016-06-24" @default.
• W1999838991 creator A5000623764 @default.
• W1999838991 creator A5002605346 @default.
• W1999838991 creator A5008084240 @default.
• W1999838991 creator A5017142587 @default.
• W1999838991 creator A5018660599 @default.
• W1999838991 creator A5033185543 @default.
• W1999838991 creator A5034549366 @default.
• W1999838991 creator A5040719852 @default.
• W1999838991 creator A5045814434 @default.
• W1999838991 creator A5056643974 @default.
• W1999838991 creator A5061729793 @default.
• W1999838991 date "2015-01-23" @default.
• W1999838991 modified "2023-10-18" @default.
• W1999838991 title "Genome-wide immunity studies in the rabbit: transcriptome variations in peripheral blood mononuclear cells after in vitro stimulation by LPS or PMA-Ionomycin" @default.
• W1999838991 cites W1470168957 @default.
• W1999838991 cites W1496078232 @default.
• W1999838991 cites W1500925296 @default.
• W1999838991 cites W1515760574 @default.
• W1999838991 cites W1529074782 @default.
• W1999838991 cites W1532855220 @default.
• W1999838991 cites W1811874414 @default.
• W1999838991 cites W1959413965 @default.
• W1999838991 cites W1966013800 @default.
• W1999838991 cites W1967369695 @default.
• W1999838991 cites W1968483701 @default.
• W1999838991 cites W1972963835 @default.
• W1999838991 cites W1977107428 @default.
• W1999838991 cites W1977577669 @default.
• W1999838991 cites W1978643157 @default.
• W1999838991 cites W1980108423 @default.
• W1999838991 cites W1987099691 @default.
• W1999838991 cites W1990834285 @default.
• W1999838991 cites W1991429888 @default.
• W1999838991 cites W1996255033 @default.
• W1999838991 cites W1999387338 @default.
• W1999838991 cites W1999418442 @default.
• W1999838991 cites W2004916218 @default.
• W1999838991 cites W2005043395 @default.
• W1999838991 cites W2006249975 @default.
• W1999838991 cites W2009964522 @default.
• W1999838991 cites W2011006768 @default.
• W1999838991 cites W2017437933 @default.
• W1999838991 cites W2018199713 @default.
• W1999838991 cites W2021568554 @default.
• W1999838991 cites W2024439539 @default.
• W1999838991 cites W2024636269 @default.
• W1999838991 cites W2029884594 @default.
• W1999838991 cites W2034653971 @default.
• W1999838991 cites W2035356471 @default.
• W1999838991 cites W2052998574 @default.
• W1999838991 cites W2053617622 @default.
• W1999838991 cites W2061234125 @default.
• W1999838991 cites W2066749848 @default.
• W1999838991 cites W2071419608 @default.
• W1999838991 cites W2076052954 @default.
• W1999838991 cites W2080207536 @default.
• W1999838991 cites W2085339088 @default.
• W1999838991 cites W2085369865 @default.
• W1999838991 cites W2096793039 @default.
• W1999838991 cites W2099426062 @default.
• W1999838991 cites W2100864093 @default.
• W1999838991 cites W2102083592 @default.
• W1999838991 cites W2105084426 @default.
• W1999838991 cites W2105664859 @default.
• W1999838991 cites W2109168637 @default.
• W1999838991 cites W2109453374 @default.
• W1999838991 cites W2117564575 @default.
• W1999838991 cites W2118030557 @default.
• W1999838991 cites W2126795224 @default.
• W1999838991 cites W2129319296 @default.
• W1999838991 cites W2133506458 @default.
• W1999838991 cites W2134193575 @default.
• W1999838991 cites W2135806909 @default.
• W1999838991 cites W2136971069 @default.
• W1999838991 cites W2140368744 @default.
• W1999838991 cites W2142415307 @default.
• W1999838991 cites W2143916054 @default.
• W1999838991 cites W2144040053 @default.
• W1999838991 cites W2150987680 @default.
• W1999838991 cites W2160610558 @default.
• W1999838991 cites W2163203554 @default.
• W1999838991 cites W2169876265 @default.
• W1999838991 cites W2170526373 @default.
• W1999838991 cites W2331688327 @default.
• W1999838991 cites W3023923910 @default.
• W1999838991 cites W4211030691 @default.
• W1999838991 doi "https://doi.org/10.1186/s12864-015-1218-9" @default.
• W1999838991 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4326531" @default.
• W1999838991 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25613284" @default.
• W1999838991 hasPublicationYear "2015" @default.
• W1999838991 type Work @default.
• W1999838991 sameAs 1999838991 @default.
• W1999838991 citedByCount "21" @default.
• W1999838991 countsByYear W19998389912016 @default.
• W1999838991 countsByYear W19998389912017 @default.
• W1999838991 countsByYear W19998389912019 @default.
• W1999838991 countsByYear W19998389912020 @default.
• W1999838991 countsByYear W19998389912021 @default.

• next