Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999858141> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W1999858141 endingPage "40" @default.
- W1999858141 startingPage "35" @default.
- W1999858141 abstract "Context: Hilsha is one of the most popular fish in Bangladesh. Biochemical analysis of Hilsha egg revealed that it is quite a healthful food. A specific class of proteins called lectins is partially purified from this source.Objectives: To carry the nutritional analysis of Hilsha eggs and to isolate sugar-specific lectins from the animal source by applying effective purification techniques.Materials and Methods: The moisture, ash, protein, lipid, polysaccharide, free sugar, cholesterol, calcium, phosphorus and iron contents were determined by the conventional methods and the lectins were isolated by using the affinity chromatographic technique. The effect of temperature, pH and metal ions were observed by performing the hemagglutination assay.Results: The moisture, ash, protein, lipid, polysaccharide, free sugar, cholesterol, calcium, phosphorus and iron contents were 34-, 2.4-, 32.59-, 28.35-, 0.036-, 0.059-, 0.40-, 0.178-, 0.193- and 0.136%, respectively. The partially purified crude protein by affinity chromatography from Hilsha eggs agglutinated rabbit erythrocytes and the hemagglutinating activity is inhibited by 25mM Raffinose followed by 100 mM of glucose, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine and D-galactosamine-HCl each. In the presence of the chelating agent EDTA, the lectin lost its activity completely. Thermal and pH inactivation assay of the lectin indicates that the activity is highest at 30 to 40°C and at the pH 5 to 9, respectively. Conclusion: Hilsha fish eggs can be recommended as a nutritious food and as well as can be regarded as a source of animal lectins, a group of sugar-binding proteins.Key words: Nutritional analysis; Hemagglutination assay; Temperature; pH; Tenualosa ilisha.DOI: 10.3329/jbs.v17i0.7097J. bio-sci. 17: 35-40, 2009" @default.
- W1999858141 created "2016-06-24" @default.
- W1999858141 creator A5019942660 @default.
- W1999858141 creator A5021014908 @default.
- W1999858141 creator A5055769899 @default.
- W1999858141 creator A5073926489 @default.
- W1999858141 date "1970-01-01" @default.
- W1999858141 modified "2023-09-27" @default.
- W1999858141 title "Biochemical Analysis and Physico Chemical Stability of a Partially Purified Lectin from Hilsha Eggs" @default.
- W1999858141 cites W1560923897 @default.
- W1999858141 cites W1992056420 @default.
- W1999858141 cites W2012871799 @default.
- W1999858141 cites W2018922839 @default.
- W1999858141 cites W2025651022 @default.
- W1999858141 cites W2037767703 @default.
- W1999858141 cites W2039578735 @default.
- W1999858141 cites W2055800251 @default.
- W1999858141 cites W2088666955 @default.
- W1999858141 cites W2115227037 @default.
- W1999858141 cites W2152311269 @default.
- W1999858141 cites W2166086221 @default.
- W1999858141 cites W3141624786 @default.
- W1999858141 cites W3141819817 @default.
- W1999858141 doi "https://doi.org/10.3329/jbs.v17i0.7097" @default.
- W1999858141 hasPublicationYear "1970" @default.
- W1999858141 type Work @default.
- W1999858141 sameAs 1999858141 @default.
- W1999858141 citedByCount "2" @default.
- W1999858141 countsByYear W19998581412013 @default.
- W1999858141 countsByYear W19998581412016 @default.
- W1999858141 crossrefType "journal-article" @default.
- W1999858141 hasAuthorship W1999858141A5019942660 @default.
- W1999858141 hasAuthorship W1999858141A5021014908 @default.
- W1999858141 hasAuthorship W1999858141A5055769899 @default.
- W1999858141 hasAuthorship W1999858141A5073926489 @default.
- W1999858141 hasBestOaLocation W19998581411 @default.
- W1999858141 hasConcept C100817775 @default.
- W1999858141 hasConcept C178790620 @default.
- W1999858141 hasConcept C179247698 @default.
- W1999858141 hasConcept C181199279 @default.
- W1999858141 hasConcept C185592680 @default.
- W1999858141 hasConcept C2777108408 @default.
- W1999858141 hasConcept C2778125326 @default.
- W1999858141 hasConcept C2778597767 @default.
- W1999858141 hasConcept C2778944976 @default.
- W1999858141 hasConcept C2779998883 @default.
- W1999858141 hasConcept C2780758401 @default.
- W1999858141 hasConcept C2781467277 @default.
- W1999858141 hasConcept C31903555 @default.
- W1999858141 hasConcept C43617362 @default.
- W1999858141 hasConcept C515207424 @default.
- W1999858141 hasConcept C519063684 @default.
- W1999858141 hasConcept C55493867 @default.
- W1999858141 hasConceptScore W1999858141C100817775 @default.
- W1999858141 hasConceptScore W1999858141C178790620 @default.
- W1999858141 hasConceptScore W1999858141C179247698 @default.
- W1999858141 hasConceptScore W1999858141C181199279 @default.
- W1999858141 hasConceptScore W1999858141C185592680 @default.
- W1999858141 hasConceptScore W1999858141C2777108408 @default.
- W1999858141 hasConceptScore W1999858141C2778125326 @default.
- W1999858141 hasConceptScore W1999858141C2778597767 @default.
- W1999858141 hasConceptScore W1999858141C2778944976 @default.
- W1999858141 hasConceptScore W1999858141C2779998883 @default.
- W1999858141 hasConceptScore W1999858141C2780758401 @default.
- W1999858141 hasConceptScore W1999858141C2781467277 @default.
- W1999858141 hasConceptScore W1999858141C31903555 @default.
- W1999858141 hasConceptScore W1999858141C43617362 @default.
- W1999858141 hasConceptScore W1999858141C515207424 @default.
- W1999858141 hasConceptScore W1999858141C519063684 @default.
- W1999858141 hasConceptScore W1999858141C55493867 @default.
- W1999858141 hasLocation W19998581411 @default.
- W1999858141 hasOpenAccess W1999858141 @default.
- W1999858141 hasPrimaryLocation W19998581411 @default.
- W1999858141 hasRelatedWork W1551923710 @default.
- W1999858141 hasRelatedWork W1966478756 @default.
- W1999858141 hasRelatedWork W1981153574 @default.
- W1999858141 hasRelatedWork W1988689615 @default.
- W1999858141 hasRelatedWork W1999858141 @default.
- W1999858141 hasRelatedWork W2011412895 @default.
- W1999858141 hasRelatedWork W2016987335 @default.
- W1999858141 hasRelatedWork W204093968 @default.
- W1999858141 hasRelatedWork W2093083869 @default.
- W1999858141 hasRelatedWork W2095128775 @default.
- W1999858141 hasVolume "17" @default.
- W1999858141 isParatext "false" @default.
- W1999858141 isRetracted "false" @default.
- W1999858141 magId "1999858141" @default.
- W1999858141 workType "article" @default.