Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999888946> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W1999888946 endingPage "S22" @default.
- W1999888946 startingPage "S22" @default.
- W1999888946 abstract "Viruses in the human gut have been linked to health and disease. Deciphering the gut virome is dependent on metagenomic sequencing of the virus-like particles (VLPs) purified from the fecal specimens. A major limitation of conventional viral metagenomic sequencing is the low recoverability of viral genomes from the metagenomic dataset.To develop an optimal method for viral amplification and metagenomic sequencing for maximizing the recovery of viral genomes.We performed parallel virus enrichment and DNA extraction to generate ∼ 30 viral DNA samples from each of 5 fresh fecal specimens and conducted the experiments including 1) optimizing the cycle number for high-fidelity enzyme-based PCR amplification, 2) evaluating the reproducibility of the optimally whole viral metagenomic experimental process, 3) evaluating the reliability of multiple displacement amplification (MDA), 4) testing the capability of long-read sequencing for improving viral metagenomic assembly, and 5) comparing the differences between viral metagenomic and bulk metagenomic approaches.Our results revealed that the optimal cycle number for PCR amplification is 15. We verified the reliability of MDA and the effectiveness of long-read sequencing. Based on our optimized results, we generated 151 high-quality viruses using the dataset combined from short-read and long-read sequencing. Genomic analysis of these viruses found that most (60.3%) of them were previously unknown and showed a remarkable diversity of viral functions, especially the existence of 206 viral auxiliary metabolic genes. Finally, we uncovered significant differences in the efficiency and coverage of viral identification between viral metagenomic and bulk metagenomic approaches.Our study demonstrates the potential of optimized experiment and sequencing strategies in uncovering viral genomes from fecal specimens, which will facilitate future research about the genome-level characterization of complex viral communities." @default.
- W1999888946 created "2016-06-24" @default.
- W1999888946 creator A5001504884 @default.
- W1999888946 creator A5007051275 @default.
- W1999888946 creator A5011645172 @default.
- W1999888946 creator A5027877793 @default.
- W1999888946 creator A5044984230 @default.
- W1999888946 creator A5058777914 @default.
- W1999888946 creator A5073495025 @default.
- W1999888946 date "2009-09-01" @default.
- W1999888946 modified "2023-09-25" @default.
- W1999888946 title "PIV-16 RSV epidemiological surveillance in Belgium reveals a novel 6 nucleotide insertion into the G glycoprotein in circulating GB13 strains" @default.
- W1999888946 doi "https://doi.org/10.1016/s1386-6532(09)70112-x" @default.
- W1999888946 hasPublicationYear "2009" @default.
- W1999888946 type Work @default.
- W1999888946 sameAs 1999888946 @default.
- W1999888946 citedByCount "0" @default.
- W1999888946 crossrefType "journal-article" @default.
- W1999888946 hasAuthorship W1999888946A5001504884 @default.
- W1999888946 hasAuthorship W1999888946A5007051275 @default.
- W1999888946 hasAuthorship W1999888946A5011645172 @default.
- W1999888946 hasAuthorship W1999888946A5027877793 @default.
- W1999888946 hasAuthorship W1999888946A5044984230 @default.
- W1999888946 hasAuthorship W1999888946A5058777914 @default.
- W1999888946 hasAuthorship W1999888946A5073495025 @default.
- W1999888946 hasConcept C104317684 @default.
- W1999888946 hasConcept C132917006 @default.
- W1999888946 hasConcept C141231307 @default.
- W1999888946 hasConcept C15151743 @default.
- W1999888946 hasConcept C159047783 @default.
- W1999888946 hasConcept C190743605 @default.
- W1999888946 hasConcept C2522874641 @default.
- W1999888946 hasConcept C49105822 @default.
- W1999888946 hasConcept C51679486 @default.
- W1999888946 hasConcept C54355233 @default.
- W1999888946 hasConcept C70721500 @default.
- W1999888946 hasConcept C78063203 @default.
- W1999888946 hasConcept C86803240 @default.
- W1999888946 hasConcept C8769409 @default.
- W1999888946 hasConceptScore W1999888946C104317684 @default.
- W1999888946 hasConceptScore W1999888946C132917006 @default.
- W1999888946 hasConceptScore W1999888946C141231307 @default.
- W1999888946 hasConceptScore W1999888946C15151743 @default.
- W1999888946 hasConceptScore W1999888946C159047783 @default.
- W1999888946 hasConceptScore W1999888946C190743605 @default.
- W1999888946 hasConceptScore W1999888946C2522874641 @default.
- W1999888946 hasConceptScore W1999888946C49105822 @default.
- W1999888946 hasConceptScore W1999888946C51679486 @default.
- W1999888946 hasConceptScore W1999888946C54355233 @default.
- W1999888946 hasConceptScore W1999888946C70721500 @default.
- W1999888946 hasConceptScore W1999888946C78063203 @default.
- W1999888946 hasConceptScore W1999888946C86803240 @default.
- W1999888946 hasConceptScore W1999888946C8769409 @default.
- W1999888946 hasLocation W19998889461 @default.
- W1999888946 hasOpenAccess W1999888946 @default.
- W1999888946 hasPrimaryLocation W19998889461 @default.
- W1999888946 hasRelatedWork W2042198562 @default.
- W1999888946 hasRelatedWork W2097029864 @default.
- W1999888946 hasRelatedWork W2155652097 @default.
- W1999888946 hasRelatedWork W2255506873 @default.
- W1999888946 hasRelatedWork W3023436389 @default.
- W1999888946 hasRelatedWork W3043668926 @default.
- W1999888946 hasRelatedWork W3157490637 @default.
- W1999888946 hasRelatedWork W3216950677 @default.
- W1999888946 hasRelatedWork W4226125465 @default.
- W1999888946 hasRelatedWork W4281800584 @default.
- W1999888946 hasVolume "46" @default.
- W1999888946 isParatext "false" @default.
- W1999888946 isRetracted "false" @default.
- W1999888946 magId "1999888946" @default.
- W1999888946 workType "article" @default.