FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999928783> ?p ?o ?g. }

• W1999928783 endingPage "24" @default.
• W1999928783 startingPage "13" @default.
• W1999928783 abstract "In the present work we demonstrate the application of a commercial biosensor instrument (BIACORE 1000, Biacore AB, Uppsala) for the detection of antibodies against the hepatitis A virus (HAV) in human serum samples using linear and branched synthetic peptides related to the VP3 capsid protein of HAV. We also studied the conformation of the synthetic peptides by circular dichroism (CD) in order to analyse the changes in secondary structure of the constructs that could influence their recognition by antibodies. Linear and dimeric VP3(110-121) multiple antigen peptides (MAP) were the most sensitive and appropriate for serological studies of serum from HAV infected patients using BIACORE. Immobilization of tetrameric MAPs via amine groups apparently failed to preserve the active conformation of the peptide epitope since it led to lower antibody binding compared to linear and dimeric peptides. The CD analysis showed that the tetrameric MAP constructs tend to adopt a beta-sheet structure due to intermolecular aggregation, which limits epitope accessibility. Our results demonstrate the value of biospecific interaction analysis technology using synthetic peptides for the diagnosis of acute hepatitis A." @default.
• W1999928783 created "2016-06-24" @default.
• W1999928783 creator A5003889865 @default.
• W1999928783 creator A5042431642 @default.
• W1999928783 creator A5070094861 @default.
• W1999928783 creator A5079349606 @default.
• W1999928783 date "2000-12-01" @default.
• W1999928783 modified "2023-10-14" @default.
• W1999928783 title "Assessment of synthetic peptides for hepatitis A diagnosis using biosensor technology" @default.
• W1999928783 cites W1509339083 @default.
• W1999928783 cites W1813958293 @default.
• W1999928783 cites W1966824783 @default.
• W1999928783 cites W1968568577 @default.
• W1999928783 cites W1975969275 @default.
• W1999928783 cites W1982504538 @default.
• W1999928783 cites W1987602232 @default.
• W1999928783 cites W1993524878 @default.
• W1999928783 cites W1996145449 @default.
• W1999928783 cites W2006481557 @default.
• W1999928783 cites W2008049698 @default.
• W1999928783 cites W2020340473 @default.
• W1999928783 cites W2039873895 @default.
• W1999928783 cites W2043737927 @default.
• W1999928783 cites W2045638180 @default.
• W1999928783 cites W2047217757 @default.
• W1999928783 cites W2047337009 @default.
• W1999928783 cites W2065528557 @default.
• W1999928783 cites W2078615464 @default.
• W1999928783 cites W2080270383 @default.
• W1999928783 cites W2082497628 @default.
• W1999928783 cites W2108388671 @default.
• W1999928783 cites W2120408949 @default.
• W1999928783 cites W2123631149 @default.
• W1999928783 cites W2124286063 @default.
• W1999928783 cites W2145572269 @default.
• W1999928783 cites W2153292240 @default.
• W1999928783 cites W2212129211 @default.
• W1999928783 cites W4360758432 @default.
• W1999928783 doi "https://doi.org/10.1016/s0022-1759(00)00295-7" @default.
• W1999928783 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11121543" @default.
• W1999928783 hasPublicationYear "2000" @default.
• W1999928783 type Work @default.
• W1999928783 sameAs 1999928783 @default.
• W1999928783 citedByCount "43" @default.
• W1999928783 countsByYear W19999287832012 @default.
• W1999928783 countsByYear W19999287832013 @default.
• W1999928783 countsByYear W19999287832014 @default.
• W1999928783 countsByYear W19999287832015 @default.
• W1999928783 countsByYear W19999287832016 @default.
• W1999928783 countsByYear W19999287832017 @default.
• W1999928783 countsByYear W19999287832020 @default.
• W1999928783 countsByYear W19999287832021 @default.
• W1999928783 crossrefType "journal-article" @default.
• W1999928783 hasAuthorship W1999928783A5003889865 @default.
• W1999928783 hasAuthorship W1999928783A5042431642 @default.
• W1999928783 hasAuthorship W1999928783A5070094861 @default.
• W1999928783 hasAuthorship W1999928783A5079349606 @default.
• W1999928783 hasConcept C104317684 @default.
• W1999928783 hasConcept C133571119 @default.
• W1999928783 hasConcept C153911025 @default.
• W1999928783 hasConcept C159047783 @default.
• W1999928783 hasConcept C159654299 @default.
• W1999928783 hasConcept C160756335 @default.
• W1999928783 hasConcept C185592680 @default.
• W1999928783 hasConcept C195616568 @default.
• W1999928783 hasConcept C202878990 @default.
• W1999928783 hasConcept C203014093 @default.
• W1999928783 hasConcept C2522874641 @default.
• W1999928783 hasConcept C2779281246 @default.
• W1999928783 hasConcept C2781241564 @default.
• W1999928783 hasConcept C3019983190 @default.
• W1999928783 hasConcept C55493867 @default.
• W1999928783 hasConcept C86803240 @default.
• W1999928783 hasConceptScore W1999928783C104317684 @default.
• W1999928783 hasConceptScore W1999928783C133571119 @default.
• W1999928783 hasConceptScore W1999928783C153911025 @default.
• W1999928783 hasConceptScore W1999928783C159047783 @default.
• W1999928783 hasConceptScore W1999928783C159654299 @default.
• W1999928783 hasConceptScore W1999928783C160756335 @default.
• W1999928783 hasConceptScore W1999928783C185592680 @default.
• W1999928783 hasConceptScore W1999928783C195616568 @default.
• W1999928783 hasConceptScore W1999928783C202878990 @default.
• W1999928783 hasConceptScore W1999928783C203014093 @default.
• W1999928783 hasConceptScore W1999928783C2522874641 @default.
• W1999928783 hasConceptScore W1999928783C2779281246 @default.
• W1999928783 hasConceptScore W1999928783C2781241564 @default.
• W1999928783 hasConceptScore W1999928783C3019983190 @default.
• W1999928783 hasConceptScore W1999928783C55493867 @default.
• W1999928783 hasConceptScore W1999928783C86803240 @default.
• W1999928783 hasIssue "1-2" @default.
• W1999928783 hasLocation W19999287831 @default.
• W1999928783 hasLocation W19999287832 @default.
• W1999928783 hasOpenAccess W1999928783 @default.
• W1999928783 hasPrimaryLocation W19999287831 @default.
• W1999928783 hasRelatedWork W1966990229 @default.
• W1999928783 hasRelatedWork W1980469726 @default.
• W1999928783 hasRelatedWork W2000352908 @default.
• W1999928783 hasRelatedWork W2054129679 @default.

• next