FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999969892> ?p ?o ?g. }

• W1999969892 endingPage "357" @default.
• W1999969892 startingPage "347" @default.
• W1999969892 abstract "Ebolaviruses are causative agents of lethal hemorrhagic fever in humans and nonhuman primates. Among the filoviruses characterized thus far, Reston Ebola virus (REBOV) is the only Ebola virus that is nonpathogenic to humans despite the fact that REBOV can cause lethal disease in nonhuman primates. Previous studies also suggest that REBOV is less effective at inhibiting host innate immune responses than Zaire Ebola virus (ZEBOV) or Marburg virus. Virally encoded VP35 protein is critical for immune suppression, but an understanding of the relative contributions of VP35 proteins from REBOV and other filoviruses is currently lacking. In order to address this question, we characterized the REBOV VP35 interferon inhibitory domain (IID) using structural, biochemical, and virological studies. These studies reveal differences in double-stranded RNA binding and interferon inhibition between the two species. These observed differences are likely due to increased stability and loss of flexibility in REBOV VP35 IID, as demonstrated by thermal shift stability assays. Consistent with this finding, the 1.71-A crystal structure of REBOV VP35 IID reveals that it is highly similar to that of ZEBOV VP35 IID, with an overall backbone r.m.s.d. of 0.64 A, but contains an additional helical element at the linker between the two subdomains of VP35 IID. Mutations near the linker, including swapping sequences between REBOV and ZEBOV, reveal that the linker sequence has limited tolerance for variability. Together with the previously solved ligand-free and double-stranded-RNA-bound forms of ZEBOV VP35 IID structures, our current studies on REBOV VP35 IID reinforce the importance of VP35 in immune suppression. Functional differences observed between REBOV and ZEBOV VP35 proteins may contribute to observed differences in pathogenicity, but these are unlikely to be the major determinant. However, the high level of similarity in structure and the low tolerance for sequence variability, coupled with the multiple critical roles played by Ebola virus VP35 proteins, highlight the viability of VP35 as a potential target for therapeutic development." @default.
• W1999969892 created "2016-06-24" @default.
• W1999969892 creator A5002400585 @default.
• W1999969892 creator A5002923427 @default.
• W1999969892 creator A5003011120 @default.
• W1999969892 creator A5016335715 @default.
• W1999969892 creator A5030646943 @default.
• W1999969892 creator A5033840109 @default.
• W1999969892 creator A5036377965 @default.
• W1999969892 creator A5054163903 @default.
• W1999969892 creator A5068032160 @default.
• W1999969892 date "2010-06-01" @default.
• W1999969892 modified "2023-10-14" @default.
• W1999969892 title "Structural and Functional Characterization of Reston Ebola Virus VP35 Interferon Inhibitory Domain" @default.
• W1999969892 cites W1539796472 @default.
• W1999969892 cites W1825594680 @default.
• W1999969892 cites W1967588904 @default.
• W1999969892 cites W1978722366 @default.
• W1999969892 cites W1992433051 @default.
• W1999969892 cites W1995323565 @default.
• W1999969892 cites W2001641653 @default.
• W1999969892 cites W2003058341 @default.
• W1999969892 cites W2006635133 @default.
• W1999969892 cites W2007178775 @default.
• W1999969892 cites W2007608885 @default.
• W1999969892 cites W2013913832 @default.
• W1999969892 cites W2015999301 @default.
• W1999969892 cites W2017003212 @default.
• W1999969892 cites W2026660010 @default.
• W1999969892 cites W2036436901 @default.
• W1999969892 cites W2038840577 @default.
• W1999969892 cites W2050361028 @default.
• W1999969892 cites W2056946199 @default.
• W1999969892 cites W2057559854 @default.
• W1999969892 cites W2059118814 @default.
• W1999969892 cites W2065939419 @default.
• W1999969892 cites W2083626719 @default.
• W1999969892 cites W2084575458 @default.
• W1999969892 cites W2092987196 @default.
• W1999969892 cites W2096701457 @default.
• W1999969892 cites W2097493124 @default.
• W1999969892 cites W2099533608 @default.
• W1999969892 cites W2100665873 @default.
• W1999969892 cites W2100827617 @default.
• W1999969892 cites W2105746066 @default.
• W1999969892 cites W2106587802 @default.
• W1999969892 cites W2106882534 @default.
• W1999969892 cites W2108486460 @default.
• W1999969892 cites W2118436036 @default.
• W1999969892 cites W2119810476 @default.
• W1999969892 cites W2122339645 @default.
• W1999969892 cites W2128782078 @default.
• W1999969892 cites W2131583337 @default.
• W1999969892 cites W2134667443 @default.
• W1999969892 cites W2136920638 @default.
• W1999969892 cites W2137587180 @default.
• W1999969892 cites W2137627132 @default.
• W1999969892 cites W2138838687 @default.
• W1999969892 cites W2143550120 @default.
• W1999969892 cites W2144081223 @default.
• W1999969892 cites W2155409207 @default.
• W1999969892 cites W2160695434 @default.
• W1999969892 cites W2160999690 @default.
• W1999969892 cites W2162244749 @default.
• W1999969892 cites W2164680606 @default.
• W1999969892 cites W2167972491 @default.
• W1999969892 cites W2169020166 @default.
• W1999969892 cites W2169821755 @default.
• W1999969892 doi "https://doi.org/10.1016/j.jmb.2010.04.022" @default.
• W1999969892 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2917615" @default.
• W1999969892 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20399790" @default.
• W1999969892 hasPublicationYear "2010" @default.
• W1999969892 type Work @default.
• W1999969892 sameAs 1999969892 @default.
• W1999969892 citedByCount "63" @default.
• W1999969892 countsByYear W19999698922012 @default.
• W1999969892 countsByYear W19999698922013 @default.
• W1999969892 countsByYear W19999698922014 @default.
• W1999969892 countsByYear W19999698922015 @default.
• W1999969892 countsByYear W19999698922016 @default.
• W1999969892 countsByYear W19999698922017 @default.
• W1999969892 countsByYear W19999698922018 @default.
• W1999969892 countsByYear W19999698922019 @default.
• W1999969892 countsByYear W19999698922020 @default.
• W1999969892 countsByYear W19999698922021 @default.
• W1999969892 countsByYear W19999698922022 @default.
• W1999969892 crossrefType "journal-article" @default.
• W1999969892 hasAuthorship W1999969892A5002400585 @default.
• W1999969892 hasAuthorship W1999969892A5002923427 @default.
• W1999969892 hasAuthorship W1999969892A5003011120 @default.
• W1999969892 hasAuthorship W1999969892A5016335715 @default.
• W1999969892 hasAuthorship W1999969892A5030646943 @default.
• W1999969892 hasAuthorship W1999969892A5033840109 @default.
• W1999969892 hasAuthorship W1999969892A5036377965 @default.
• W1999969892 hasAuthorship W1999969892A5054163903 @default.
• W1999969892 hasAuthorship W1999969892A5068032160 @default.
• W1999969892 hasBestOaLocation W19999698922 @default.
• W1999969892 hasConcept C136449434 @default.

• next