FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999991049> ?p ?o ?g. }

• W1999991049 endingPage "17" @default.
• W1999991049 startingPage "1" @default.
• W1999991049 abstract "Cell surface expression of the epidermal growth factor (EGF) receptor in several cell lines declines as a function of increased cell density and is associated with diminished responsiveness to EGF. However, the mechanism whereby this density-induced down regulation of receptors occurs has not been discerned. In the present study the distribution of the EGF receptor in A-431 cells as a function of cell density using (1) two polyclonal antibodies raised against peptide specific sequences of the EGF receptor that recognize either the cytoplasmic or extracellular domains of the receptor, respectively, and (2) biotinylated EGF, a specific probe for the cell surface receptor is now investigated. Immunolocalization of the receptor using the polyclonal antibodies or the biotin-EGF revealed that the receptor was homogeneously distributed on the cell surface of individual cells, or in cells plated at low density. In contrast, as cell density increased, prominent EGF immunoreactivity and biotin-EGF staining became limited to the periphery of the cells, at sites of cell-cell apposition, and was characterized by a honeycomb pattern, typical of a basolateral distribution. The effects of low Ca++ treatment, known to cause cells to round up and detach from one another, on EGF receptor distribution in cells at high cell density were then examined. Confocal microscopy of immunostained preparations revealed that incubation of high density cultures in Ca(++)-free media for as little as 10 min restored the homogeneous distribution of the EGF receptor and resulted in strong intracellular staining. Three-dimensional reconstruction of serial optical sections revealed that redistribution of the EGF receptor following low Ca++ treatment involved a heretofore undetected 'ruffling', an immunostaining pattern characterized by stripes of intense fluorescence signal interspersed with complete absence of fluorescence. Next, cell-cell adhesion was disrupted with antisera to the cell adhesion molecule E-cadherin. Although the antisera caused cells to detach from one another, eventually leading to cell rounding and redistribution of the EGF receptor, the receptor 'ruffling' immunostaining pattern rendered by the low Ca++ treatment was not detected. These results suggest that an association may exist between the plasma membrane EGF receptor distribution, density-induced EGF receptor down regulation, and the growth effects of low Ca++ observed in previous studies." @default.
• W1999991049 created "2016-06-24" @default.
• W1999991049 creator A5050973833 @default.
• W1999991049 creator A5051224199 @default.
• W1999991049 date "1993-01-01" @default.
• W1999991049 modified "2023-10-16" @default.
• W1999991049 title "Redistribution of epidermal growth factor receptor as a function of cell density, cell-cell adhesion and calcium in human (A-431) cells" @default.
• W1999991049 cites W1605235578 @default.
• W1999991049 cites W1969928750 @default.
• W1999991049 cites W1976002101 @default.
• W1999991049 cites W1981752846 @default.
• W1999991049 cites W1987190223 @default.
• W1999991049 cites W1988127405 @default.
• W1999991049 cites W1991185441 @default.
• W1999991049 cites W1992139451 @default.
• W1999991049 cites W1995337883 @default.
• W1999991049 cites W2007171380 @default.
• W1999991049 cites W2012294594 @default.
• W1999991049 cites W2029082867 @default.
• W1999991049 cites W2038085058 @default.
• W1999991049 cites W2039328394 @default.
• W1999991049 cites W2046633126 @default.
• W1999991049 cites W2051747697 @default.
• W1999991049 cites W2056293980 @default.
• W1999991049 cites W2067188408 @default.
• W1999991049 cites W2069314886 @default.
• W1999991049 cites W2084072745 @default.
• W1999991049 cites W2095520922 @default.
• W1999991049 cites W2100837269 @default.
• W1999991049 cites W2111817213 @default.
• W1999991049 cites W2115048429 @default.
• W1999991049 cites W2115453867 @default.
• W1999991049 cites W2146447599 @default.
• W1999991049 cites W2159930645 @default.
• W1999991049 cites W2160696263 @default.
• W1999991049 doi "https://doi.org/10.1016/0040-8166(93)90061-o" @default.
• W1999991049 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8470090" @default.
• W1999991049 hasPublicationYear "1993" @default.
• W1999991049 type Work @default.
• W1999991049 sameAs 1999991049 @default.
• W1999991049 citedByCount "12" @default.
• W1999991049 crossrefType "journal-article" @default.
• W1999991049 hasAuthorship W1999991049A5050973833 @default.
• W1999991049 hasAuthorship W1999991049A5051224199 @default.
• W1999991049 hasBestOaLocation W19999910491 @default.
• W1999991049 hasConcept C1491633281 @default.
• W1999991049 hasConcept C153911025 @default.
• W1999991049 hasConcept C170493617 @default.
• W1999991049 hasConcept C197462201 @default.
• W1999991049 hasConcept C2776362946 @default.
• W1999991049 hasConcept C55493867 @default.
• W1999991049 hasConcept C86803240 @default.
• W1999991049 hasConcept C95444343 @default.
• W1999991049 hasConceptScore W1999991049C1491633281 @default.
• W1999991049 hasConceptScore W1999991049C153911025 @default.
• W1999991049 hasConceptScore W1999991049C170493617 @default.
• W1999991049 hasConceptScore W1999991049C197462201 @default.
• W1999991049 hasConceptScore W1999991049C2776362946 @default.
• W1999991049 hasConceptScore W1999991049C55493867 @default.
• W1999991049 hasConceptScore W1999991049C86803240 @default.
• W1999991049 hasConceptScore W1999991049C95444343 @default.
• W1999991049 hasIssue "1" @default.
• W1999991049 hasLocation W19999910491 @default.
• W1999991049 hasLocation W19999910492 @default.
• W1999991049 hasOpenAccess W1999991049 @default.
• W1999991049 hasPrimaryLocation W19999910491 @default.
• W1999991049 hasRelatedWork W1983939044 @default.
• W1999991049 hasRelatedWork W1994376676 @default.
• W1999991049 hasRelatedWork W1995425733 @default.
• W1999991049 hasRelatedWork W2005747351 @default.
• W1999991049 hasRelatedWork W2017530429 @default.
• W1999991049 hasRelatedWork W2064762154 @default.
• W1999991049 hasRelatedWork W2125589729 @default.
• W1999991049 hasRelatedWork W222782741 @default.
• W1999991049 hasRelatedWork W2740082710 @default.
• W1999991049 hasRelatedWork W4244463151 @default.
• W1999991049 hasVolume "25" @default.
• W1999991049 isParatext "false" @default.
• W1999991049 isRetracted "false" @default.
• W1999991049 magId "1999991049" @default.
• W1999991049 workType "article" @default.