Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999994030> ?p ?o ?g. }
- W1999994030 endingPage "307" @default.
- W1999994030 startingPage "298" @default.
- W1999994030 abstract "Regulated transgene expression may reduce transgene-specific and genotoxic risks associated with gene therapy. To prove this concept, we have investigated the suitability of doxycycline (Dox)-inducible human cytidine deaminase (hCDD) overexpression from lentiviral vectors to mediate effective myeloprotection while circumventing the lymphotoxicity observed with constitutive CDD activity. Rapid Dox-mediated transgene induction associated with a 6–17-fold increase in drug resistance was observed in 32D and primary murine bone marrow (BM) cells. Moreover, robust Dox-regulated transgene expression in the entire haematopoietic system was demonstrated for primary and secondary recipients of hCDD-transduced R26-M2rtTA transgenic BM cells. Furthermore, mice were significantly protected from myelosuppressive chemotherapy as evidenced by accelerated recovery of granulocytes (1.9±0.6 vs 1.3±0.3, P=0.034) and platelets (883±194 vs 584±160 103 per μl, P=0.011). Minimal transgene expression in the non-induced state and no overt cellular toxicities including lymphotoxicity were detected. Thus, using a relevant murine transplant model our data provide conclusive evidence that drug-resistance transgenes can be expressed in a regulated fashion in the lymphohaematopoietic system, and that Dox-inducible systems may be used to reduce myelotoxic side effect of anticancer chemotherapy or to avoid side effects of high constitutive transgene expression." @default.
- W1999994030 created "2016-06-24" @default.
- W1999994030 creator A5001888046 @default.
- W1999994030 creator A5015829071 @default.
- W1999994030 creator A5017285480 @default.
- W1999994030 creator A5035125862 @default.
- W1999994030 creator A5037368020 @default.
- W1999994030 creator A5043204742 @default.
- W1999994030 creator A5047937141 @default.
- W1999994030 creator A5054362291 @default.
- W1999994030 creator A5066642367 @default.
- W1999994030 creator A5069241778 @default.
- W1999994030 creator A5091554256 @default.
- W1999994030 date "2012-05-17" @default.
- W1999994030 modified "2023-09-25" @default.
- W1999994030 title "Efficient in vivo regulation of cytidine deaminase expression in the haematopoietic system using a doxycycline-inducible lentiviral vector system" @default.
- W1999994030 cites W1966320426 @default.
- W1999994030 cites W1966983163 @default.
- W1999994030 cites W1970586355 @default.
- W1999994030 cites W1971047566 @default.
- W1999994030 cites W1976696463 @default.
- W1999994030 cites W1979744985 @default.
- W1999994030 cites W1983240586 @default.
- W1999994030 cites W1984317932 @default.
- W1999994030 cites W1994523215 @default.
- W1999994030 cites W1998591382 @default.
- W1999994030 cites W2000059304 @default.
- W1999994030 cites W2001670543 @default.
- W1999994030 cites W2005275397 @default.
- W1999994030 cites W2006765654 @default.
- W1999994030 cites W2007048730 @default.
- W1999994030 cites W2009078306 @default.
- W1999994030 cites W2009875479 @default.
- W1999994030 cites W2013908437 @default.
- W1999994030 cites W2023619104 @default.
- W1999994030 cites W2024157997 @default.
- W1999994030 cites W2025659537 @default.
- W1999994030 cites W2031371983 @default.
- W1999994030 cites W2045239199 @default.
- W1999994030 cites W2057436316 @default.
- W1999994030 cites W2062801650 @default.
- W1999994030 cites W2066336622 @default.
- W1999994030 cites W2068972073 @default.
- W1999994030 cites W2082632242 @default.
- W1999994030 cites W2082799445 @default.
- W1999994030 cites W2090429649 @default.
- W1999994030 cites W2107046588 @default.
- W1999994030 cites W2107278930 @default.
- W1999994030 cites W2108238886 @default.
- W1999994030 cites W2111751689 @default.
- W1999994030 cites W2111967568 @default.
- W1999994030 cites W2119324304 @default.
- W1999994030 cites W2123545304 @default.
- W1999994030 cites W2131578393 @default.
- W1999994030 cites W2134175746 @default.
- W1999994030 cites W2136482187 @default.
- W1999994030 cites W2141350766 @default.
- W1999994030 cites W2143729842 @default.
- W1999994030 cites W2147703815 @default.
- W1999994030 cites W2147859982 @default.
- W1999994030 cites W2150329966 @default.
- W1999994030 cites W2150712887 @default.
- W1999994030 cites W2154853638 @default.
- W1999994030 cites W2167523976 @default.
- W1999994030 cites W2169125054 @default.
- W1999994030 cites W2335670169 @default.
- W1999994030 cites W4236073474 @default.
- W1999994030 cites W4241358973 @default.
- W1999994030 cites W4248378708 @default.
- W1999994030 doi "https://doi.org/10.1038/gt.2012.40" @default.
- W1999994030 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22592598" @default.
- W1999994030 hasPublicationYear "2012" @default.
- W1999994030 type Work @default.
- W1999994030 sameAs 1999994030 @default.
- W1999994030 citedByCount "15" @default.
- W1999994030 countsByYear W19999940302012 @default.
- W1999994030 countsByYear W19999940302013 @default.
- W1999994030 countsByYear W19999940302014 @default.
- W1999994030 countsByYear W19999940302015 @default.
- W1999994030 countsByYear W19999940302017 @default.
- W1999994030 countsByYear W19999940302020 @default.
- W1999994030 crossrefType "journal-article" @default.
- W1999994030 hasAuthorship W1999994030A5001888046 @default.
- W1999994030 hasAuthorship W1999994030A5015829071 @default.
- W1999994030 hasAuthorship W1999994030A5017285480 @default.
- W1999994030 hasAuthorship W1999994030A5035125862 @default.
- W1999994030 hasAuthorship W1999994030A5037368020 @default.
- W1999994030 hasAuthorship W1999994030A5043204742 @default.
- W1999994030 hasAuthorship W1999994030A5047937141 @default.
- W1999994030 hasAuthorship W1999994030A5054362291 @default.
- W1999994030 hasAuthorship W1999994030A5066642367 @default.
- W1999994030 hasAuthorship W1999994030A5069241778 @default.
- W1999994030 hasAuthorship W1999994030A5091554256 @default.
- W1999994030 hasBestOaLocation W19999940301 @default.
- W1999994030 hasConcept C102230213 @default.
- W1999994030 hasConcept C104317684 @default.
- W1999994030 hasConcept C109159458 @default.
- W1999994030 hasConcept C111599444 @default.