Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000007989> ?p ?o ?g. }
- W2000007989 endingPage "182" @default.
- W2000007989 startingPage "173" @default.
- W2000007989 abstract "1. Autodegradation of yeast ribosomes is due to a 'latent' ribonuclease which is associated with the 40 S ribosomal subunit. 2. The ribonuclease was extracted in the presence of EDTA from ribosomes and purified 118-rold by protamine sulphate precipitation, (NH4)2SO4 fractionation and chromatography on DEAE-cellulose. 3. The optimum pH for this enzyme is 5 to 6.5 while the optimum temperature is 45 to 50 degrees C. Incubation for 10 min at 60 degrees C caused a reduction in enzyme activity of 70%. 4. The ribonuclease has an endonucleolytic activity against rRNA, tRNA, poly(A), poly(U) and poly(C) but does not degrade poly(G) or DNA. It hydrolyzes the homopolymers to nucleoside 3'-phosphates. 5. Zn2+, Mn2+, heparin, glutathione and p-chloromercuribenzoate inhibit the ribonuclease, while Na+, K+, EDTA and sermidine have only little or no effect. 6. It binds tightly to yeast ribosomes but only loosely to ribonuclease-free wheat germ ribosomes. 7. Polyribosomes possess less autodegradation activity than monoribosomes, isolated from the same homogenate." @default.
- W2000007989 created "2016-06-24" @default.
- W2000007989 creator A5001084531 @default.
- W2000007989 creator A5038500415 @default.
- W2000007989 creator A5055160332 @default.
- W2000007989 date "1979-11-01" @default.
- W2000007989 modified "2023-09-26" @default.
- W2000007989 title "A ribonuclease from yeast associated with the 40 S ribosomal subunit" @default.
- W2000007989 cites W1501147426 @default.
- W2000007989 cites W1505792829 @default.
- W2000007989 cites W1547215623 @default.
- W2000007989 cites W1553775471 @default.
- W2000007989 cites W158744088 @default.
- W2000007989 cites W1617336596 @default.
- W2000007989 cites W1993249093 @default.
- W2000007989 cites W2025715945 @default.
- W2000007989 cites W2027295620 @default.
- W2000007989 cites W2037722158 @default.
- W2000007989 cites W2049516788 @default.
- W2000007989 cites W205725875 @default.
- W2000007989 cites W2057586635 @default.
- W2000007989 cites W2066721283 @default.
- W2000007989 cites W2068961979 @default.
- W2000007989 cites W2084381734 @default.
- W2000007989 cites W2086710230 @default.
- W2000007989 cites W2093306471 @default.
- W2000007989 cites W2227738997 @default.
- W2000007989 cites W2412870948 @default.
- W2000007989 cites W2417550346 @default.
- W2000007989 cites W2461197907 @default.
- W2000007989 cites W2800298984 @default.
- W2000007989 cites W4235240512 @default.
- W2000007989 cites W4318358395 @default.
- W2000007989 cites W9455128 @default.
- W2000007989 cites W2063200410 @default.
- W2000007989 doi "https://doi.org/10.1016/0005-2787(79)90093-5" @default.
- W2000007989 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/41579" @default.
- W2000007989 hasPublicationYear "1979" @default.
- W2000007989 type Work @default.
- W2000007989 sameAs 2000007989 @default.
- W2000007989 citedByCount "10" @default.
- W2000007989 crossrefType "journal-article" @default.
- W2000007989 hasAuthorship W2000007989A5001084531 @default.
- W2000007989 hasAuthorship W2000007989A5038500415 @default.
- W2000007989 hasAuthorship W2000007989A5055160332 @default.
- W2000007989 hasConcept C104292427 @default.
- W2000007989 hasConcept C104317684 @default.
- W2000007989 hasConcept C111921641 @default.
- W2000007989 hasConcept C112755997 @default.
- W2000007989 hasConcept C153911025 @default.
- W2000007989 hasConcept C181199279 @default.
- W2000007989 hasConcept C185592680 @default.
- W2000007989 hasConcept C2777557582 @default.
- W2000007989 hasConcept C2778976237 @default.
- W2000007989 hasConcept C2779222958 @default.
- W2000007989 hasConcept C2780704907 @default.
- W2000007989 hasConcept C2780835560 @default.
- W2000007989 hasConcept C38062823 @default.
- W2000007989 hasConcept C55493867 @default.
- W2000007989 hasConcept C65666806 @default.
- W2000007989 hasConcept C67705224 @default.
- W2000007989 hasConcept C67905577 @default.
- W2000007989 hasConcept C86803240 @default.
- W2000007989 hasConcept C88478588 @default.
- W2000007989 hasConceptScore W2000007989C104292427 @default.
- W2000007989 hasConceptScore W2000007989C104317684 @default.
- W2000007989 hasConceptScore W2000007989C111921641 @default.
- W2000007989 hasConceptScore W2000007989C112755997 @default.
- W2000007989 hasConceptScore W2000007989C153911025 @default.
- W2000007989 hasConceptScore W2000007989C181199279 @default.
- W2000007989 hasConceptScore W2000007989C185592680 @default.
- W2000007989 hasConceptScore W2000007989C2777557582 @default.
- W2000007989 hasConceptScore W2000007989C2778976237 @default.
- W2000007989 hasConceptScore W2000007989C2779222958 @default.
- W2000007989 hasConceptScore W2000007989C2780704907 @default.
- W2000007989 hasConceptScore W2000007989C2780835560 @default.
- W2000007989 hasConceptScore W2000007989C38062823 @default.
- W2000007989 hasConceptScore W2000007989C55493867 @default.
- W2000007989 hasConceptScore W2000007989C65666806 @default.
- W2000007989 hasConceptScore W2000007989C67705224 @default.
- W2000007989 hasConceptScore W2000007989C67905577 @default.
- W2000007989 hasConceptScore W2000007989C86803240 @default.
- W2000007989 hasConceptScore W2000007989C88478588 @default.
- W2000007989 hasIssue "1" @default.
- W2000007989 hasLocation W20000079891 @default.
- W2000007989 hasLocation W20000079892 @default.
- W2000007989 hasOpenAccess W2000007989 @default.
- W2000007989 hasPrimaryLocation W20000079891 @default.
- W2000007989 hasRelatedWork W1574378429 @default.
- W2000007989 hasRelatedWork W1582656924 @default.
- W2000007989 hasRelatedWork W1981488044 @default.
- W2000007989 hasRelatedWork W1994986117 @default.
- W2000007989 hasRelatedWork W2000007989 @default.
- W2000007989 hasRelatedWork W2034653428 @default.
- W2000007989 hasRelatedWork W2072137051 @default.
- W2000007989 hasRelatedWork W2091765495 @default.
- W2000007989 hasRelatedWork W2256924391 @default.
- W2000007989 hasRelatedWork W2416109724 @default.