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- W2000109009 abstract "A method is described for using the cross-linking reagent 4′-(hydroxy-methyl)-4,5′,8-trimethylpsoralen (HMT) to map bsss paired regions and higher-order structure within RNA molecules. Applying this method to yeast tRNAPhe, we have specifically identified cross-links within the acceptor stem between U6 X U68 in the D-stem between C11 X C25 and in the Tψ-stem between U50 X C63 and U52 X C63. We have also identified a unique cross-link between U8 X C48 which are trans pyrimidines in the core region due to tertiary interactions between U8:A14 and C48:G15 The precise point of cross-linking was deduced in every case by using purine-specific U2 ribo-nuclease along with cytidine-specific CL3 ribonuclease which will anomalously cleave after photoreversed pyrimidines. The ability to map the precise point of cross-linking should prove invaluable in identifying nucleotides in close proximity within the tertiary structure of other RNA molecules." @default.
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- W2000109009 date "1984-01-01" @default.
- W2000109009 modified "2023-09-25" @default.
- W2000109009 title "Mapping of psoralen cross-linked nucleotides in RNA" @default.
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- W2000109009 doi "https://doi.org/10.1093/nar/12.7.3405" @default.
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