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- W2000140281 abstract "D-Galacturonic acid reductase, a key enzyme in ascorbate biosynthesis, was purified to homogeneity from Euglena gracilis. The enzyme was a monomer with a molecular mass of 38-39 kDa, as judged by SDS-PAGE and gel filtration. Apparently it utilized NADPH with a Km value of 62.5+/-4.5 microM and uronic acids, such as D-galacturonic acid (Km=3.79+/-0.5 mM) and D-glucuronic acid (Km=4.67+/-0.6 mM). It failed to catalyze the reverse reaction with L-galactonic acid and NADP(+). The optimal pH for the reduction of D-galacturonic acid was 7.2. The enzyme was activated 45.6% by 0.1 mM H(2)O(2), suggesting that enzyme activity is regulated by cellular redox status. No feedback regulation of the enzyme activity by L-galactono-1,4-lactone or ascorbate was observed. N-terminal amino acid sequence analysis revealed that the enzyme is closely related to the malate dehydrogenase families." @default.
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- W2000140281 date "2006-11-23" @default.
- W2000140281 modified "2023-09-27" @default.
- W2000140281 title "Functional Characterization of<scp>D</scp>-Galacturonic Acid Reductase, a Key Enzyme of the Ascorbate Biosynthesis Pathway, from<i>Euglena gracilis</i>" @default.
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- W2000140281 doi "https://doi.org/10.1271/bbb.60327" @default.
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