FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000160400> ?p ?o ?g. }

• W2000160400 endingPage "26" @default.
• W2000160400 startingPage "15" @default.
• W2000160400 abstract "<i>Background:</i><i>Penicillium chrysogenum (Penicillium notatum)</i> is a prevalent airborne <i>Penicillium</i> species. A 34-kD major IgE-reacting component from <i>P. chrysogenum</i> has been identified as an alkaline serine protease (Pen ch 13, also known as Pen n 13 before) by immunoblot and N-terminal amino acid sequence analysis. <i>Methods:</i> In the present study, Pen ch 13 was further characterized in terms of cDNA cloning, protein purification, enzymatic activity, histamine release and IgE cross-reactivity with alkaline serine protease allergens from two other prevalent fungal species – <i>P. citrinum</i> (Pen c 13) and <i>Aspergillus flavus</i> (Asp fl 13). <i>Results:</i> A 1,478-bp cDNA (Pen ch 13) that encodes a 398-amino-acid alkaline serine protease from <i>P. chrysogenum</i> was isolated. This fungal protease has pre- and pro-enzyme sequences. The previously determined N-terminal amino acid sequence of the <i>P. chrysogenum</i> 34-kD major allergen is identical to that of residues 116–125 of the cDNA. Starting from Ala116, the deduced amino acid sequence (283 residues) of the mature alkaline serine protease has a calculated molecular mass of 28.105 kD with two cysteines and two putative N-glycosylation sites. It has 83 and 49% sequence identity with the alkaline serine proteases from <i>P. citrinum</i> and <i>A. fumigatus</i>, respectively. The recombinant Pen ch 13 was recovered from inclusion bodies and isolated under denaturing condition. This recombinant protein reacted with IgE antibodies in serum from an asthmatic patient and with monoclonal antibodies (PCM8, PCM10, PCM39) that reacted with the 34-kD component from <i>P. chrysogenum</i>. The N-terminal amino acid sequence of the purified native Pen ch 13 is identical to that determined previously for the 34-kD major allergen in crude <i>P. chrysogenum</i> extracts. The purified native Pen ch 13 has proteolytic activity with casein as the substrate at pH 8.0. This enzymatic activity was inhibited by phenylmethylsulfonyl fluoride or diethylpyrocarbonate. Pen ch 13 was also able to degrade gelatin and collagen but not elastin. Basophils from 5 asthmatic patients released histamine (12–73%) when exposed to the purified Pen ch 13. In ELISA (enzyme-linked immunosorbent assay) experiments, IgE for Pen ch 13 was able to compete with purified Pen ch 13, Pen c 13 or Asp fl 13 in a dose-related manner. <i>Conclusions:</i> These results demonstrated that the 34-kD major allergen of <i>P. chrysogenum</i> is an alkaline serine protease. These results also indicated that atopic patients primarily sensitized by either of these prevalent fungal species may develop allergic symptoms by exposure to other environmental fungi due to cross-reacting IgE antibodies against this protease." @default.
• W2000160400 created "2016-06-24" @default.
• W2000160400 creator A5001890947 @default.
• W2000160400 creator A5004523590 @default.
• W2000160400 creator A5012185249 @default.
• W2000160400 creator A5014628665 @default.
• W2000160400 creator A5015160969 @default.
• W2000160400 creator A5073560676 @default.
• W2000160400 creator A5085458949 @default.
• W2000160400 date "2002-01-01" @default.
• W2000160400 modified "2023-10-10" @default.
• W2000160400 title "cDNA Cloning, Biological and Immunological Characterization of the Alkaline Serine Protease Major Allergen from <i>Penicillium chrysogenum</i>" @default.
• W2000160400 cites W1974728713 @default.
• W2000160400 cites W1982782105 @default.
• W2000160400 cites W1990209153 @default.
• W2000160400 cites W2001043603 @default.
• W2000160400 cites W2096241845 @default.
• W2000160400 doi "https://doi.org/10.1159/000048165" @default.
• W2000160400 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11893850" @default.
• W2000160400 hasPublicationYear "2002" @default.
• W2000160400 type Work @default.
• W2000160400 sameAs 2000160400 @default.
• W2000160400 citedByCount "49" @default.
• W2000160400 countsByYear W20001604002013 @default.
• W2000160400 countsByYear W20001604002014 @default.
• W2000160400 countsByYear W20001604002016 @default.
• W2000160400 countsByYear W20001604002020 @default.
• W2000160400 countsByYear W20001604002022 @default.
• W2000160400 countsByYear W20001604002023 @default.
• W2000160400 crossrefType "journal-article" @default.
• W2000160400 hasAuthorship W2000160400A5001890947 @default.
• W2000160400 hasAuthorship W2000160400A5004523590 @default.
• W2000160400 hasAuthorship W2000160400A5012185249 @default.
• W2000160400 hasAuthorship W2000160400A5014628665 @default.
• W2000160400 hasAuthorship W2000160400A5015160969 @default.
• W2000160400 hasAuthorship W2000160400A5073560676 @default.
• W2000160400 hasAuthorship W2000160400A5085458949 @default.
• W2000160400 hasConcept C104317684 @default.
• W2000160400 hasConcept C153911025 @default.
• W2000160400 hasConcept C167625842 @default.
• W2000160400 hasConcept C181199279 @default.
• W2000160400 hasConcept C185592680 @default.
• W2000160400 hasConcept C187882448 @default.
• W2000160400 hasConcept C2776142995 @default.
• W2000160400 hasConcept C2776414213 @default.
• W2000160400 hasConcept C2776714187 @default.
• W2000160400 hasConcept C2777270571 @default.
• W2000160400 hasConcept C2777807008 @default.
• W2000160400 hasConcept C31903555 @default.
• W2000160400 hasConcept C55493867 @default.
• W2000160400 hasConcept C86803240 @default.
• W2000160400 hasConceptScore W2000160400C104317684 @default.
• W2000160400 hasConceptScore W2000160400C153911025 @default.
• W2000160400 hasConceptScore W2000160400C167625842 @default.
• W2000160400 hasConceptScore W2000160400C181199279 @default.
• W2000160400 hasConceptScore W2000160400C185592680 @default.
• W2000160400 hasConceptScore W2000160400C187882448 @default.
• W2000160400 hasConceptScore W2000160400C2776142995 @default.
• W2000160400 hasConceptScore W2000160400C2776414213 @default.
• W2000160400 hasConceptScore W2000160400C2776714187 @default.
• W2000160400 hasConceptScore W2000160400C2777270571 @default.
• W2000160400 hasConceptScore W2000160400C2777807008 @default.
• W2000160400 hasConceptScore W2000160400C31903555 @default.
• W2000160400 hasConceptScore W2000160400C55493867 @default.
• W2000160400 hasConceptScore W2000160400C86803240 @default.
• W2000160400 hasIssue "1" @default.
• W2000160400 hasLocation W20001604001 @default.
• W2000160400 hasLocation W20001604002 @default.
• W2000160400 hasOpenAccess W2000160400 @default.
• W2000160400 hasPrimaryLocation W20001604001 @default.
• W2000160400 hasRelatedWork W1972560754 @default.
• W2000160400 hasRelatedWork W2008305082 @default.
• W2000160400 hasRelatedWork W2069967524 @default.
• W2000160400 hasRelatedWork W2159092200 @default.
• W2000160400 hasRelatedWork W2367312412 @default.
• W2000160400 hasRelatedWork W2883654761 @default.
• W2000160400 hasRelatedWork W3035812378 @default.
• W2000160400 hasRelatedWork W3048569867 @default.
• W2000160400 hasRelatedWork W4200192018 @default.
• W2000160400 hasRelatedWork W4309353051 @default.
• W2000160400 hasVolume "127" @default.
• W2000160400 isParatext "false" @default.
• W2000160400 isRetracted "false" @default.
• W2000160400 magId "2000160400" @default.
• W2000160400 workType "article" @default.