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- W2000167181 abstract "Testis cells of the toad Xenopus laevis were dissociated with collagenase and the cell suspension was enriched for steroidogenic cells by Percoll gradients. Results suggested that cells should be preincubated during a 6-h period before stimulation with gonadotropin. Our results indicate that a 2-h incubation period with gonadotropin was necessary to obtain a significant response. Furthermore, the cells can be maintained in a functional state longer than mammalian testis cells. Different gonadotropins were used to stimulate androgen production, and their effects were compared in both dissociated cells and testicular explants. Cells were more sensitive to luteinizing hormone (LH) and follicle stimulating hormone (FSH) than the explants (ED50 LH = 0.041 ± 0.003 μg for cells and 0.097 ± 0.002 μg for explants: ED50 FSH = 0.41 ± 0.03 μg for cells and 0.63 ± 0.03 μg for explants). Moreover, human chorionic gonadotropin (hCG) which only stimulates testicular explants at high doses, failed to stimulate the androgen production of dissociated cells; this indicates a low sensitivity of amphibian testis to hCG and a possible damaging effect of collagenase on the receptors of isolated cells." @default.
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- W2000167181 date "1988-02-01" @default.
- W2000167181 modified "2023-09-27" @default.
- W2000167181 title "Gonadotropin action on androgen synthesis in short-term incubation of explants and dispersed cells of the testis ofXenopus laevis" @default.
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- W2000167181 doi "https://doi.org/10.1002/jez.1402450207" @default.
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