Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000167322> ?p ?o ?g. }
- W2000167322 endingPage "97" @default.
- W2000167322 startingPage "85" @default.
- W2000167322 abstract "The effects of epidermal growth factor on high density primary cultures of fetal (embryonic day 17) rat septal cells were examined. Under serum-free conditions, the continuous exposure of these cultures to epidermal growth factor for seven days significantly decreased choline acetyltransferase (EC 2.3.1.6) activity in a dose-dependent manner. Maximal decreases were observed from 1 to 10 ng/ml epidermal growth factor. This effect was completely abolished by the addition of anti-epidermal growth factor antibodies. The epidermal growth factor-mediated decrease in choline acetyltransferase activity was culture-time dependent, being first detectable after five days of factor application and may likely represent an inhibition of the spontaneous increase in enzyme activity that occurs with time in culture. Concomitant with changes in enzyme activity, epidermal growth factor produced a significant and proportional decrease in the number of acetylcholinesterase-positive neurons. This decrease in acetylcholinesterase-positive cells did not reflect a decrease in cholinergic cell survival as nerve growth factor could restore the number of acetylcholinesterase-positive neurons in epidermal growth factor-treated cultures to control levels. Furthermore, in these high-density cultures, epidermal growth factor did not affect general neuronal survival, while it did produce an increase in the number and intensity of glial fibrillary acidic protein-immunoreactive astroglia as well as in the number of macrophage-like cells. The proliferative response of these non-neuronal cells to epidermal growth factor, as assessed by [3H]thymidine incorporation, was evident after three days of epidermal growth factor application, persisted thereafter, and could be antagonized by the inclusion of the antimitotic 5-fluorodeoxyuridine. Furthermore, 5-fluorodeoxyuridine completely blocked the epidermal growth factor-mediated decrease in choline acetyltransferase activity. However, when epidermal growth factor was tested in pure glial cultures, it only directly induced proliferation of astrocytes. These results suggest that the proliferative response of either one or both of these glial cell types in the mixed cultures may be indirectly affecting cholinergic cell expression." @default.
- W2000167322 created "2016-06-24" @default.
- W2000167322 creator A5031343335 @default.
- W2000167322 creator A5033125462 @default.
- W2000167322 creator A5038964959 @default.
- W2000167322 creator A5048517642 @default.
- W2000167322 date "1992-09-01" @default.
- W2000167322 modified "2023-10-04" @default.
- W2000167322 title "Epidermal growth factor affects both glia and cholinergic neurons in septal cell cultures" @default.
- W2000167322 cites W1486790573 @default.
- W2000167322 cites W1492445976 @default.
- W2000167322 cites W1545662949 @default.
- W2000167322 cites W1562089313 @default.
- W2000167322 cites W1634194046 @default.
- W2000167322 cites W1646206347 @default.
- W2000167322 cites W1673416974 @default.
- W2000167322 cites W1964160902 @default.
- W2000167322 cites W1966864860 @default.
- W2000167322 cites W1968120180 @default.
- W2000167322 cites W1973092712 @default.
- W2000167322 cites W1973238388 @default.
- W2000167322 cites W1979529470 @default.
- W2000167322 cites W1981432754 @default.
- W2000167322 cites W1982064952 @default.
- W2000167322 cites W1982339250 @default.
- W2000167322 cites W1985792428 @default.
- W2000167322 cites W1990432605 @default.
- W2000167322 cites W1991201219 @default.
- W2000167322 cites W1992598058 @default.
- W2000167322 cites W1995802332 @default.
- W2000167322 cites W1999368094 @default.
- W2000167322 cites W2001644248 @default.
- W2000167322 cites W2002429726 @default.
- W2000167322 cites W2002475153 @default.
- W2000167322 cites W2003366456 @default.
- W2000167322 cites W2004472372 @default.
- W2000167322 cites W2004831331 @default.
- W2000167322 cites W2010398513 @default.
- W2000167322 cites W2011237803 @default.
- W2000167322 cites W2013580165 @default.
- W2000167322 cites W2020446758 @default.
- W2000167322 cites W2025811127 @default.
- W2000167322 cites W2033317917 @default.
- W2000167322 cites W2033577739 @default.
- W2000167322 cites W2033699178 @default.
- W2000167322 cites W2035156191 @default.
- W2000167322 cites W2037724671 @default.
- W2000167322 cites W2050329524 @default.
- W2000167322 cites W2051945664 @default.
- W2000167322 cites W2057272187 @default.
- W2000167322 cites W2057410920 @default.
- W2000167322 cites W2059994807 @default.
- W2000167322 cites W2063784792 @default.
- W2000167322 cites W2064739683 @default.
- W2000167322 cites W2068384471 @default.
- W2000167322 cites W2070574092 @default.
- W2000167322 cites W2075464971 @default.
- W2000167322 cites W2076870940 @default.
- W2000167322 cites W2079395100 @default.
- W2000167322 cites W2081396951 @default.
- W2000167322 cites W2082658535 @default.
- W2000167322 cites W2088650980 @default.
- W2000167322 cites W2088730075 @default.
- W2000167322 cites W2090246247 @default.
- W2000167322 cites W2094913385 @default.
- W2000167322 cites W2095240672 @default.
- W2000167322 cites W2095873344 @default.
- W2000167322 cites W2102796334 @default.
- W2000167322 cites W2105469110 @default.
- W2000167322 cites W2127471857 @default.
- W2000167322 doi "https://doi.org/10.1016/0306-4522(92)90383-d" @default.
- W2000167322 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1407562" @default.
- W2000167322 hasPublicationYear "1992" @default.
- W2000167322 type Work @default.
- W2000167322 sameAs 2000167322 @default.
- W2000167322 citedByCount "22" @default.
- W2000167322 countsByYear W20001673222020 @default.
- W2000167322 crossrefType "journal-article" @default.
- W2000167322 hasAuthorship W2000167322A5031343335 @default.
- W2000167322 hasAuthorship W2000167322A5033125462 @default.
- W2000167322 hasAuthorship W2000167322A5038964959 @default.
- W2000167322 hasAuthorship W2000167322A5048517642 @default.
- W2000167322 hasConcept C104966671 @default.
- W2000167322 hasConcept C126322002 @default.
- W2000167322 hasConcept C134018914 @default.
- W2000167322 hasConcept C170493617 @default.
- W2000167322 hasConcept C181199279 @default.
- W2000167322 hasConcept C198399640 @default.
- W2000167322 hasConcept C22885893 @default.
- W2000167322 hasConcept C2775960820 @default.
- W2000167322 hasConcept C2776362946 @default.
- W2000167322 hasConcept C2778423431 @default.
- W2000167322 hasConcept C2778816929 @default.
- W2000167322 hasConcept C54355233 @default.
- W2000167322 hasConcept C55493867 @default.
- W2000167322 hasConcept C71924100 @default.
- W2000167322 hasConcept C81885089 @default.
- W2000167322 hasConcept C86803240 @default.