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- W2000171693 abstract "A reconstructed monolayer was formed using epithelial cells from normal mouse kidney to investigate the hormonal effect on phosphate transport by the renal cells. The cells, when cultured on a Millipore filter, formed a monolayer with an apical negative transepithelial potential of 8.4 +/- 0.4 mV. When radioactive phosphate was added onto the apical surface of the monolayer (corresponding to the luminal surface of a renal tubule), the phosphate was transported through the cell layer to the basolateral surface (corresponding to the peritubular surface of a renal tubule). This transport process was saturable, energy-dependent, and inhibited by 2,4-dinitrophenol or ouabain. Dose-dependent parathyroid hormone-induced inhibition (73% of the control) was also evident in this system. Similar inhibition (69% of the control) was observed with DBcAMP. Thus, monolayers reconstructed from cultured mouse kidney cells show characteristics similar to those of renal tubules." @default.
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- W2000171693 date "1987-01-01" @default.
- W2000171693 modified "2023-09-23" @default.
- W2000171693 title "Phosphate Transport by Reconstructed Monolayers from Cultured Mouse Kidney Cells." @default.
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- W2000171693 doi "https://doi.org/10.1507/endocrj1954.34.595" @default.
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