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• W2000173129 abstract "Isopycnic banding of small-plaque polyoma virus in CsCl produced four bands at densities 1.28, 1.29, 1.33, and 1.34. Examination of these fractions in the electron microscope revealed the upper band to consist of 48 mμ shells incompletely filled with PTA. These were interpreted as being 48 mμ shells with an internal 38 mμ shell. The second band consisted of three sizes of separated, capsomered shells 48, 38, and 22 mμ in diameter which are believed to consist of 72, 32, and 12 “capsomeres”, respectively. The lower two bands were not morphologically different, consisting of complete virus particles. A 26 mμ shell of DNA was shown in complete particles by uranyl acetate staining, and a 38 mμ capsomere shell was demonstrated in situ between the 26 mμ DNA shell and a ring of residual stain between the 48 and 38 mμ shells. These observations suggest that the polyoma virus may be constructed (from interior to exterior) of a 22 mμ shell of capsomeres, a thin shell of DNA, a 38 mμ shell of capsomeres and a 48 mμ protein shell. A fifth layer of phospholipid covers some particles to a diameter of about 60 mμ A similar picture of three sizes of spherical particles was seen with SV40 virus. Filamentous, capsomered forms were found in large number only in KBr “cushion” fractions; apparently filaments were destroyed by CsCl. Filaments also occurred in three general sizes, 22, 38, and 48 mμ Possible roles of filamentous forms in virus replication are discussed." @default.
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• W2000173129 date "1967-07-01" @default.
• W2000173129 modified "2023-09-25" @default.
• W2000173129 title "Electron microscopic observations on multiple polyoma virus-related particles" @default.
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• W2000173129 doi "https://doi.org/10.1016/0042-6822(67)90288-7" @default.
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