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• W2000183670 abstract "We have sequenced genomic fragments which encode the N-terminus of the TIMELESS (TIM) clock protein in Drosophila simulans and D.yakuba . We observe that in these two species, the initiating methionine appears to lie downstream of the one proposed to encode the translational start in D.melanogaster , thereby truncating the N-terminus by 23 amino acids. We then sequenced the corresponding 5′ fragment in a number of D.melanogaster individuals from different strains. We observed a polymorphism which strongly suggests that the originally proposed start site cannot be utilised in some individuals, and that these flies will initiate translation of TIM at the downstream ATG. Given the current interest in TIM regulation in D.melanogaster , it is important to correctly define the N-terminus in this species." @default.
• W2000183670 created "2016-06-24" @default.
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• W2000183670 date "1997-02-01" @default.
• W2000183670 modified "2023-09-23" @default.
• W2000183670 title "Conceptual translation of timeless reveals alternative initiating methionines in Drosophila" @default.
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• W2000183670 doi "https://doi.org/10.1093/nar/25.3.455" @default.
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