Matches in SemOpenAlex for { <https://semopenalex.org/work/W200018936> ?p ?o ?g. }
- W200018936 abstract "Abstract We have generated a homogeneous population of recombinant interleukin-2 (rIL-2)-stimulated effector cells termed adherent lymphokine-activated killer cells (A-LAK) from peripheral blood mononuclear cells (PBMNC) of 14 normal individuals and tested the effect of A-LAK cells on autologous hematopoietic bone marrow (BM) progenitor growth. Enrichment of A-LAK from PBMNC depended on the propensity of A-LAK precursors to adhere to plastic and proliferate in the presence of rIL-2. The resultant population had the morphologic appearance of large granular lymphocytes, and the majority of cells (73% +/- 4%) expressed the CD56+/CD3- phenotype associated with rIL-2-stimulated natural killer (NK) cells. The A-LAK population had potent lytic activity in chromium release assays against both NK-sensitive (K562) and NK-resistant (Raji) targets. When BM mononuclear cells (BMMNC) were coincubated with autologous A-LAK and rIL-2 (1,000 U/mL) added at the start of culture, dose-dependent suppression of burst-forming unit-erythroid (BFU-E) and colony-forming unit mix (CFU-MIX) colony growth was observed at effector to target ratios (E:T) ranging from 0.25:1 to 5:1 (maximal suppression BFU-E = 85% +/- 6%; CFU-MIX = 95% +/- 3%). This suppression was rIL-2 dose-dependent, and no suppression was seen in the absence of rIL-2. Depletion of BM monocytes and T lymphocytes did not alter A-LAK suppression of progenitors coincubated with A-LAK cells. Addition of polyclonal neutralizing antibodies against both interferon-gamma (IFN- gamma) and tumor necrosis facto alpha (TNF-alpha) to the coincubation culture completely abrogated the suppressive effect of A-LAK on BFU-E and CFU-MIX colony growth while each neutralizing antibody used alone had intermediate effects. In contrast to coincubation studies, 36 hours of preincubation of A-LAK cells with autologous BM (E:T 2.2:1) and rIL- 2 (1,000 U/mL) followed by plating of preincubated BM cells in hematopoietic progenitor culture produced significant suppression of day 14 BFU-E (47% +/- 5%), but spared the more primitive CFU-MIX (7% +/- 9%), suggesting a divergent effect of A-LAK cells on hematopoietic progenitors at different stages of differentiation. Addition of neutralizing antibodies against IFN-gamma and TNF-alpha in preincubation failed to abrogate the suppressive effect of A-LAK on BFU- E colony growth, suggesting that this suppression occurs by a different mechanism than that seen in coincubation studies. Previous studies have demonstrated that the A-LAK population has cytotoxic and proliferative advantages over other killer cell populations.(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
- W200018936 created "2016-06-24" @default.
- W200018936 creator A5027151969 @default.
- W200018936 creator A5030197044 @default.
- W200018936 creator A5071051461 @default.
- W200018936 date "1991-06-01" @default.
- W200018936 modified "2023-10-01" @default.
- W200018936 title "Adherent lymphokine-activated killer cells suppress autologous human normal bone marrow progenitors" @default.
- W200018936 cites W116162363 @default.
- W200018936 cites W1504038693 @default.
- W200018936 cites W1552784950 @default.
- W200018936 cites W1600381474 @default.
- W200018936 cites W1606856960 @default.
- W200018936 cites W1619723454 @default.
- W200018936 cites W164432801 @default.
- W200018936 cites W1657394268 @default.
- W200018936 cites W1753372963 @default.
- W200018936 cites W186797036 @default.
- W200018936 cites W1969740606 @default.
- W200018936 cites W1983299898 @default.
- W200018936 cites W201918455 @default.
- W200018936 cites W2043369632 @default.
- W200018936 cites W2088617902 @default.
- W200018936 cites W2100045859 @default.
- W200018936 cites W2104066024 @default.
- W200018936 cites W2109258274 @default.
- W200018936 cites W2116996002 @default.
- W200018936 cites W2156330412 @default.
- W200018936 cites W2164606734 @default.
- W200018936 cites W2169580029 @default.
- W200018936 cites W2198368977 @default.
- W200018936 cites W2411331388 @default.
- W200018936 cites W2415776103 @default.
- W200018936 cites W2416035589 @default.
- W200018936 cites W2420411937 @default.
- W200018936 cites W2468160379 @default.
- W200018936 cites W2179145689 @default.
- W200018936 doi "https://doi.org/10.1182/blood.v77.11.2389.2389" @default.
- W200018936 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1903991" @default.
- W200018936 hasPublicationYear "1991" @default.
- W200018936 type Work @default.
- W200018936 sameAs 200018936 @default.
- W200018936 citedByCount "22" @default.
- W200018936 countsByYear W2000189362013 @default.
- W200018936 crossrefType "journal-article" @default.
- W200018936 hasAuthorship W200018936A5027151969 @default.
- W200018936 hasAuthorship W200018936A5030197044 @default.
- W200018936 hasAuthorship W200018936A5071051461 @default.
- W200018936 hasBestOaLocation W2000189361 @default.
- W200018936 hasConcept C109159458 @default.
- W200018936 hasConcept C129374314 @default.
- W200018936 hasConcept C153911025 @default.
- W200018936 hasConcept C154317977 @default.
- W200018936 hasConcept C167672396 @default.
- W200018936 hasConcept C201750760 @default.
- W200018936 hasConcept C202751555 @default.
- W200018936 hasConcept C203014093 @default.
- W200018936 hasConcept C2780007613 @default.
- W200018936 hasConcept C28328180 @default.
- W200018936 hasConcept C28651165 @default.
- W200018936 hasConcept C2908647359 @default.
- W200018936 hasConcept C49382859 @default.
- W200018936 hasConcept C502942594 @default.
- W200018936 hasConcept C55493867 @default.
- W200018936 hasConcept C71924100 @default.
- W200018936 hasConcept C86803240 @default.
- W200018936 hasConcept C8891405 @default.
- W200018936 hasConcept C95444343 @default.
- W200018936 hasConcept C99454951 @default.
- W200018936 hasConceptScore W200018936C109159458 @default.
- W200018936 hasConceptScore W200018936C129374314 @default.
- W200018936 hasConceptScore W200018936C153911025 @default.
- W200018936 hasConceptScore W200018936C154317977 @default.
- W200018936 hasConceptScore W200018936C167672396 @default.
- W200018936 hasConceptScore W200018936C201750760 @default.
- W200018936 hasConceptScore W200018936C202751555 @default.
- W200018936 hasConceptScore W200018936C203014093 @default.
- W200018936 hasConceptScore W200018936C2780007613 @default.
- W200018936 hasConceptScore W200018936C28328180 @default.
- W200018936 hasConceptScore W200018936C28651165 @default.
- W200018936 hasConceptScore W200018936C2908647359 @default.
- W200018936 hasConceptScore W200018936C49382859 @default.
- W200018936 hasConceptScore W200018936C502942594 @default.
- W200018936 hasConceptScore W200018936C55493867 @default.
- W200018936 hasConceptScore W200018936C71924100 @default.
- W200018936 hasConceptScore W200018936C86803240 @default.
- W200018936 hasConceptScore W200018936C8891405 @default.
- W200018936 hasConceptScore W200018936C95444343 @default.
- W200018936 hasConceptScore W200018936C99454951 @default.
- W200018936 hasLocation W2000189361 @default.
- W200018936 hasOpenAccess W200018936 @default.
- W200018936 hasPrimaryLocation W2000189361 @default.
- W200018936 hasRelatedWork W116162363 @default.
- W200018936 hasRelatedWork W1475256780 @default.
- W200018936 hasRelatedWork W1504038693 @default.
- W200018936 hasRelatedWork W1520159469 @default.
- W200018936 hasRelatedWork W1547437950 @default.
- W200018936 hasRelatedWork W1556867369 @default.
- W200018936 hasRelatedWork W1579926439 @default.
- W200018936 hasRelatedWork W175201022 @default.