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- W2000195816 abstract "We have studied the global changes which are induced in exponentially growing cultures of Escherichia coli during treatment with 7-methoxy-2-nitronaphtho[2,1-b]furan (R7000), a very potent mutagen and inducer of the SOS response. A two-dimensional analysis of the proteins synthesized in the presence of this agent was performed. In a strain deficient in SOS induction, the expression of 24 polypeptides was found to be affected by this treatment: 14 corresponded to proteins known to be implicated in different stress responses, particularly chaperones such as DnaK and GroEL. The variation of another protein was detected for the first time in this study: transketolase I, an enzyme of intermediary metabolism which was characterized in the present work by microsequencing. In parallel, the expression of 9 other proteins, still unidentified, was modified by this nitrofuran. They represent potential candidates involved in metabolic and DNA lesions repair specific of R7000 action." @default.
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- W2000195816 date "1996-02-01" @default.
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- W2000195816 title "Global response of Escherichia coli cells to a treatment with 7-methoxy-2-nitronaphtho[2,1-b]furan (R7000), an extremely potent mutagen" @default.
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- W2000195816 doi "https://doi.org/10.1016/0027-5107(95)00178-6" @default.
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