Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000200541> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2000200541 endingPage "553" @default.
- W2000200541 startingPage "545" @default.
- W2000200541 abstract "To define the role of phosphatidylserine-induced conformational changes in prothrombin activation during blood coagulation, a recombinant bovine prothrombin was constructed, characterized and shown to have a globally native-like conformation. We introduced a cysteine to replace the penultimate residue (Gly581) of a previously constructed active site mutant, and expressed the double mutant in Chinese hamster ovary cells at the level of 0.6 μg/ml of cell culture medium. Specific labeling with fluorescein maleimide was accomplished by limited reduction with dithiothreitol to free the engineered cysteine while maintaining the native-like functional properties of the molecule. The average stoichiometry of labeling was 0.84 probe/protein. The location of the probe at the C-terminus was confirmed by proteolysis by native thrombin, by Taipan venom, and by carboxypeptidase Y. Both the double mutant and labeled prothrombin could be activated by snake venoms and the prothrombinase but, as expected, the double mutant meizothrombin did not autolyze as does native meizothrombin. Thus, for the first time, a native-like but specifically labeled prothrombin has been constructed. This molecule will be an essential tool for elucidating the structural role of membranes during prothrombin activation. In addition, the methods described might be usefully applied to labeling of an odd, engineered cysteine in other disulfide bond-containing proteins." @default.
- W2000200541 created "2016-06-24" @default.
- W2000200541 creator A5010382944 @default.
- W2000200541 creator A5011933651 @default.
- W2000200541 creator A5029116912 @default.
- W2000200541 date "1996-01-01" @default.
- W2000200541 modified "2023-09-23" @default.
- W2000200541 title "Construction, properties and specific fluorescent labeling of a bovine prothrombin mutant engineered with a free C-terminal cysteine" @default.
- W2000200541 doi "https://doi.org/10.1093/protein/9.6.545" @default.
- W2000200541 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8862556" @default.
- W2000200541 hasPublicationYear "1996" @default.
- W2000200541 type Work @default.
- W2000200541 sameAs 2000200541 @default.
- W2000200541 citedByCount "1" @default.
- W2000200541 crossrefType "journal-article" @default.
- W2000200541 hasAuthorship W2000200541A5010382944 @default.
- W2000200541 hasAuthorship W2000200541A5011933651 @default.
- W2000200541 hasAuthorship W2000200541A5029116912 @default.
- W2000200541 hasBestOaLocation W20002005411 @default.
- W2000200541 hasConcept C104317684 @default.
- W2000200541 hasConcept C112516734 @default.
- W2000200541 hasConcept C143065580 @default.
- W2000200541 hasConcept C170493617 @default.
- W2000200541 hasConcept C175656101 @default.
- W2000200541 hasConcept C181199279 @default.
- W2000200541 hasConcept C185592680 @default.
- W2000200541 hasConcept C203014093 @default.
- W2000200541 hasConcept C2777292125 @default.
- W2000200541 hasConcept C2777824588 @default.
- W2000200541 hasConcept C2779201268 @default.
- W2000200541 hasConcept C55493867 @default.
- W2000200541 hasConcept C86803240 @default.
- W2000200541 hasConcept C89560881 @default.
- W2000200541 hasConceptScore W2000200541C104317684 @default.
- W2000200541 hasConceptScore W2000200541C112516734 @default.
- W2000200541 hasConceptScore W2000200541C143065580 @default.
- W2000200541 hasConceptScore W2000200541C170493617 @default.
- W2000200541 hasConceptScore W2000200541C175656101 @default.
- W2000200541 hasConceptScore W2000200541C181199279 @default.
- W2000200541 hasConceptScore W2000200541C185592680 @default.
- W2000200541 hasConceptScore W2000200541C203014093 @default.
- W2000200541 hasConceptScore W2000200541C2777292125 @default.
- W2000200541 hasConceptScore W2000200541C2777824588 @default.
- W2000200541 hasConceptScore W2000200541C2779201268 @default.
- W2000200541 hasConceptScore W2000200541C55493867 @default.
- W2000200541 hasConceptScore W2000200541C86803240 @default.
- W2000200541 hasConceptScore W2000200541C89560881 @default.
- W2000200541 hasIssue "6" @default.
- W2000200541 hasLocation W20002005411 @default.
- W2000200541 hasLocation W20002005412 @default.
- W2000200541 hasOpenAccess W2000200541 @default.
- W2000200541 hasPrimaryLocation W20002005411 @default.
- W2000200541 hasRelatedWork W1981290157 @default.
- W2000200541 hasRelatedWork W1990583841 @default.
- W2000200541 hasRelatedWork W2000200541 @default.
- W2000200541 hasRelatedWork W2003585815 @default.
- W2000200541 hasRelatedWork W2004385217 @default.
- W2000200541 hasRelatedWork W2028357718 @default.
- W2000200541 hasRelatedWork W2039213678 @default.
- W2000200541 hasRelatedWork W2055074397 @default.
- W2000200541 hasRelatedWork W2162200231 @default.
- W2000200541 hasRelatedWork W2342466069 @default.
- W2000200541 hasVolume "9" @default.
- W2000200541 isParatext "false" @default.
- W2000200541 isRetracted "false" @default.
- W2000200541 magId "2000200541" @default.
- W2000200541 workType "article" @default.