FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000200948> ?p ?o ?g. }

• W2000200948 endingPage "654" @default.
• W2000200948 startingPage "645" @default.
• W2000200948 abstract "The aim of this study was to investigate the involvement of the serine/threonine protein kinase AKT (also called protein kinase B) in the control of meiosis of porcine denuded oocytes (DOs) matured in vitro . Western blot analysis revealed that the two principal AKT phosphorylation sites, Ser473 and Thr308, are phosphorylated at different stages of meiosis. In freshly isolated germinal vesicle (GV)-stage DOs, Ser473 was already phosphorylated. After the onset of oocyte maturation, the intensity of the Ser473 phosphorylation increased, however, which declined sharply when DOs underwent GV breakdown (GVBD) and remained at low levels in metaphase I- and II-stage (MI- and MII-stage). In contrast, phosphorylation of Thr308 was increased by the time of GVBD and reached maximum at MI-stage. A peak of AKT activity was noticed around GVBD and activity of AKT declined at MI-stage. To assess the role of AKT during meiosis, porcine DOs were cultured in 50 μM SH-6, a specific inhibitor of AKT. In SH-6-treated DOs, GVBD was not inhibited; on the contrary, a significant acceleration of meiosis resumption was observed. The dynamics of the Ser473 phosphorylation was not affected; however, phosphorylation of Thr308 was reduced, AKT activity was diminished at the time of GVBD, and meiotic progression was arrested in early MI-stage. Moreover, the activity of the cyclin-dependent kinase 1 (CDK1) and MAP kinase declined when SH-6-treated DOs underwent GVBD, indicating that AKT activity is involved in the regulation of CDK1 and MAP kinase. These results suggest that activity of AKT is not essential for induction of GVBD in porcine oocytes but plays a substantial role during progression of meiosis to MI/MII-stage." @default.
• W2000200948 created "2016-06-24" @default.
• W2000200948 creator A5024877710 @default.
• W2000200948 creator A5052255710 @default.
• W2000200948 creator A5052600113 @default.
• W2000200948 creator A5069398226 @default.
• W2000200948 creator A5076054999 @default.
• W2000200948 date "2009-10-01" @default.
• W2000200948 modified "2023-10-18" @default.
• W2000200948 title "AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes" @default.
• W2000200948 cites W129960861 @default.
• W2000200948 cites W138154681 @default.
• W2000200948 cites W1726025741 @default.
• W2000200948 cites W1969857578 @default.
• W2000200948 cites W1970735844 @default.
• W2000200948 cites W1973837499 @default.
• W2000200948 cites W1975461167 @default.
• W2000200948 cites W1978570830 @default.
• W2000200948 cites W1997611649 @default.
• W2000200948 cites W2010051771 @default.
• W2000200948 cites W2014518511 @default.
• W2000200948 cites W2015641516 @default.
• W2000200948 cites W2019872821 @default.
• W2000200948 cites W2024716827 @default.
• W2000200948 cites W2038893545 @default.
• W2000200948 cites W2039144927 @default.
• W2000200948 cites W2042945054 @default.
• W2000200948 cites W2050110074 @default.
• W2000200948 cites W2053112494 @default.
• W2000200948 cites W2056511007 @default.
• W2000200948 cites W2058138221 @default.
• W2000200948 cites W2062934426 @default.
• W2000200948 cites W2063381828 @default.
• W2000200948 cites W2069244888 @default.
• W2000200948 cites W2069478525 @default.
• W2000200948 cites W2072203210 @default.
• W2000200948 cites W2072283416 @default.
• W2000200948 cites W2074903637 @default.
• W2000200948 cites W2083615478 @default.
• W2000200948 cites W2089226493 @default.
• W2000200948 cites W2089407342 @default.
• W2000200948 cites W2092214723 @default.
• W2000200948 cites W2092335032 @default.
• W2000200948 cites W2097586379 @default.
• W2000200948 cites W2099403410 @default.
• W2000200948 cites W2100739884 @default.
• W2000200948 cites W2102560485 @default.
• W2000200948 cites W2113780564 @default.
• W2000200948 cites W2126718734 @default.
• W2000200948 cites W2128519375 @default.
• W2000200948 cites W2137399388 @default.
• W2000200948 cites W2141933025 @default.
• W2000200948 cites W2143225526 @default.
• W2000200948 cites W2159630851 @default.
• W2000200948 cites W2161180058 @default.
• W2000200948 cites W41535097 @default.
• W2000200948 cites W4238567307 @default.
• W2000200948 cites W4249117478 @default.
• W2000200948 cites W4252455789 @default.
• W2000200948 cites W86393948 @default.
• W2000200948 doi "https://doi.org/10.1530/rep-08-0461" @default.
• W2000200948 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19633130" @default.
• W2000200948 hasPublicationYear "2009" @default.
• W2000200948 type Work @default.
• W2000200948 sameAs 2000200948 @default.
• W2000200948 citedByCount "39" @default.
• W2000200948 countsByYear W20002009482012 @default.
• W2000200948 countsByYear W20002009482013 @default.
• W2000200948 countsByYear W20002009482014 @default.
• W2000200948 countsByYear W20002009482015 @default.
• W2000200948 countsByYear W20002009482016 @default.
• W2000200948 countsByYear W20002009482017 @default.
• W2000200948 countsByYear W20002009482018 @default.
• W2000200948 countsByYear W20002009482019 @default.
• W2000200948 countsByYear W20002009482020 @default.
• W2000200948 countsByYear W20002009482021 @default.
• W2000200948 countsByYear W20002009482022 @default.
• W2000200948 countsByYear W20002009482023 @default.
• W2000200948 crossrefType "journal-article" @default.
• W2000200948 hasAuthorship W2000200948A5024877710 @default.
• W2000200948 hasAuthorship W2000200948A5052255710 @default.
• W2000200948 hasAuthorship W2000200948A5052600113 @default.
• W2000200948 hasAuthorship W2000200948A5069398226 @default.
• W2000200948 hasAuthorship W2000200948A5076054999 @default.
• W2000200948 hasBestOaLocation W20002009481 @default.
• W2000200948 hasConcept C11960822 @default.
• W2000200948 hasConcept C120504264 @default.
• W2000200948 hasConcept C184235292 @default.
• W2000200948 hasConcept C185592680 @default.
• W2000200948 hasConcept C190283241 @default.
• W2000200948 hasConcept C196843134 @default.
• W2000200948 hasConcept C2776690073 @default.
• W2000200948 hasConcept C29537977 @default.
• W2000200948 hasConcept C2994119143 @default.
• W2000200948 hasConcept C55493867 @default.
• W2000200948 hasConcept C62478195 @default.
• W2000200948 hasConcept C75217442 @default.
• W2000200948 hasConcept C86554907 @default.

• next