Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000203872> ?p ?o ?g. }
- W2000203872 endingPage "6540" @default.
- W2000203872 startingPage "6529" @default.
- W2000203872 abstract "We have recently shown that p-cyanophenylalanine (Cnf) and a thioamide can be used as a minimally perturbing Förster resonant energy transfer (FRET) pair to monitor protein conformation. We have also shown that thioamide analogues of natural amino acids can be incorporated into full-sized proteins through native chemical ligation. For intermolecular studies with Cnf/thioamide FRET pairs, Cnf can be incorporated into proteins expressed in Escherichia coli through unnatural amino acid mutagenesis using a Cnf-specific tRNA synthetase. For intramolecular studies, a Cnf-labeled protein fragment can be expressed in E. coli and then ligated to a thioamide-labeled peptide synthesized on solid phase. This combination of methods allows for rapid access to double-labeled proteins with a minimum of unnecessary chemical synthesis. We demonstrate the utility of this approach by studying the binding of peptides to the protein calmodulin and by determining the orientation of the N- and C-termini in the amyloidogenic protein α-synuclein." @default.
- W2000203872 created "2016-06-24" @default.
- W2000203872 creator A5028662408 @default.
- W2000203872 creator A5076043843 @default.
- W2000203872 creator A5082657976 @default.
- W2000203872 creator A5083263090 @default.
- W2000203872 date "2013-04-17" @default.
- W2000203872 modified "2023-10-13" @default.
- W2000203872 title "Labeling Proteins with Fluorophore/Thioamide Förster Resonant Energy Transfer Pairs by Combining Unnatural Amino Acid Mutagenesis and Native Chemical Ligation" @default.
- W2000203872 cites W1527671059 @default.
- W2000203872 cites W1963908898 @default.
- W2000203872 cites W1964790454 @default.
- W2000203872 cites W1966288583 @default.
- W2000203872 cites W1968780370 @default.
- W2000203872 cites W1969733268 @default.
- W2000203872 cites W1970743827 @default.
- W2000203872 cites W1973980288 @default.
- W2000203872 cites W1977267085 @default.
- W2000203872 cites W1977954945 @default.
- W2000203872 cites W1978932447 @default.
- W2000203872 cites W1983754329 @default.
- W2000203872 cites W1984701286 @default.
- W2000203872 cites W1986580794 @default.
- W2000203872 cites W1986826865 @default.
- W2000203872 cites W1988949963 @default.
- W2000203872 cites W1993145018 @default.
- W2000203872 cites W1993309394 @default.
- W2000203872 cites W1993432598 @default.
- W2000203872 cites W1993650648 @default.
- W2000203872 cites W1995191747 @default.
- W2000203872 cites W1997907471 @default.
- W2000203872 cites W2002336386 @default.
- W2000203872 cites W2004243083 @default.
- W2000203872 cites W2007635840 @default.
- W2000203872 cites W2015442731 @default.
- W2000203872 cites W2016875890 @default.
- W2000203872 cites W2017690104 @default.
- W2000203872 cites W2021025396 @default.
- W2000203872 cites W2021977313 @default.
- W2000203872 cites W2027525036 @default.
- W2000203872 cites W2029479609 @default.
- W2000203872 cites W2030401406 @default.
- W2000203872 cites W2030447262 @default.
- W2000203872 cites W2030490793 @default.
- W2000203872 cites W2030661378 @default.
- W2000203872 cites W2031836120 @default.
- W2000203872 cites W2037720445 @default.
- W2000203872 cites W2041174313 @default.
- W2000203872 cites W2044289704 @default.
- W2000203872 cites W2046014998 @default.
- W2000203872 cites W2048803110 @default.
- W2000203872 cites W2049587523 @default.
- W2000203872 cites W2049595108 @default.
- W2000203872 cites W2051070645 @default.
- W2000203872 cites W2051644112 @default.
- W2000203872 cites W2053547709 @default.
- W2000203872 cites W2058259501 @default.
- W2000203872 cites W2059912289 @default.
- W2000203872 cites W2060019534 @default.
- W2000203872 cites W2075793841 @default.
- W2000203872 cites W2080751623 @default.
- W2000203872 cites W2083377383 @default.
- W2000203872 cites W2083397106 @default.
- W2000203872 cites W2085095736 @default.
- W2000203872 cites W2089768800 @default.
- W2000203872 cites W2090324725 @default.
- W2000203872 cites W2094134509 @default.
- W2000203872 cites W2095729147 @default.
- W2000203872 cites W2097985410 @default.
- W2000203872 cites W2101995697 @default.
- W2000203872 cites W2105199969 @default.
- W2000203872 cites W2109155760 @default.
- W2000203872 cites W2110333921 @default.
- W2000203872 cites W2120597250 @default.
- W2000203872 cites W2123248324 @default.
- W2000203872 cites W2131522485 @default.
- W2000203872 cites W2137250659 @default.
- W2000203872 cites W2141617805 @default.
- W2000203872 cites W2144737808 @default.
- W2000203872 cites W2146189740 @default.
- W2000203872 cites W2146361928 @default.
- W2000203872 cites W2147565550 @default.
- W2000203872 cites W2157680389 @default.
- W2000203872 cites W2160165232 @default.
- W2000203872 cites W2163377459 @default.
- W2000203872 cites W2167373328 @default.
- W2000203872 cites W2218155072 @default.
- W2000203872 cites W2312564121 @default.
- W2000203872 cites W2491458126 @default.
- W2000203872 doi "https://doi.org/10.1021/ja4005943" @default.
- W2000203872 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3721677" @default.
- W2000203872 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23594264" @default.
- W2000203872 hasPublicationYear "2013" @default.
- W2000203872 type Work @default.
- W2000203872 sameAs 2000203872 @default.
- W2000203872 citedByCount "81" @default.
- W2000203872 countsByYear W20002038722013 @default.
- W2000203872 countsByYear W20002038722014 @default.