FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000212768> ?p ?o ?g. }

• W2000212768 endingPage "930" @default.
• W2000212768 startingPage "925" @default.
• W2000212768 abstract "Abstract Periplasmic secretion has been used in attempts to construct an efficient whole‐cell biocatalyst with greatly reduced diffusion limitations. Previously, we developed recombinant Escherichia coli that express organophosphorus hydrolase (OPH) in the periplasmic space using the twin‐arginine translocation (Tat) pathway to degrade environmental toxic organophosphate compounds. This system has the advantage of secreting protein into the periplasm after folding in the cytoplasm. However, when OPH was expressed with a Tat signal sequence in E. coli, we found that the predominant OPH was an insoluble premature form in the cytoplasm, and thus, the whole‐cell OPH activity was significantly lower than its cell lysate activity. In this work, we, for the first time, used a molecular chaperone coexpression strategy to enhance whole‐cell OPH activity by improving the periplasmic translocation of soluble OPH. We found that the effect of GroEL‐GroES (GroEL/ES) assistance on the periplasmic localization of OPH was secretory pathway dependent. We observed a significant increase in the amount of soluble mature OPH when cytoplasmic GroEL/ES was expressed; this increase in the amount of mature OPH might be due to enhanced OPH folding in the cytoplasm. Importantly, the whole‐cell OPH activity of the chaperone–coexpressing cells was ∼5.5‐fold greater at 12 h after induction than that of cells that did not express the chaperone as a result of significant Tat‐based periplasmic translocation of OPH in the chaperone–coexpressing cells. Collectively, these data suggest that molecular chaperones significantly enhance the whole‐cell activity of periplasmic OPH‐secreting cells, yielding an effective whole‐cell biocatalyst system with highly reduced diffusion limitations. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 28: 925–930, 2012" @default.
• W2000212768 created "2016-06-24" @default.
• W2000212768 creator A5002850502 @default.
• W2000212768 creator A5006781936 @default.
• W2000212768 creator A5018500712 @default.
• W2000212768 creator A5029926741 @default.
• W2000212768 creator A5044324950 @default.
• W2000212768 creator A5059704494 @default.
• W2000212768 creator A5073662332 @default.
• W2000212768 creator A5076488840 @default.
• W2000212768 creator A5083066637 @default.
• W2000212768 date "2012-06-08" @default.
• W2000212768 modified "2023-10-17" @default.
• W2000212768 title "Coexpression of molecular chaperone enhances activity and export of organophosphorus hydrolase in Escherichia coli" @default.
• W2000212768 cites W1528251237 @default.
• W2000212768 cites W1546001194 @default.
• W2000212768 cites W1557173992 @default.
• W2000212768 cites W1581713598 @default.
• W2000212768 cites W1966082253 @default.
• W2000212768 cites W1982307427 @default.
• W2000212768 cites W1991170357 @default.
• W2000212768 cites W2024055045 @default.
• W2000212768 cites W2028858498 @default.
• W2000212768 cites W2037598833 @default.
• W2000212768 cites W2041469703 @default.
• W2000212768 cites W2045985595 @default.
• W2000212768 cites W2055078937 @default.
• W2000212768 cites W2060435488 @default.
• W2000212768 cites W2060640124 @default.
• W2000212768 cites W2073516784 @default.
• W2000212768 cites W2076102277 @default.
• W2000212768 cites W2076102941 @default.
• W2000212768 cites W2078048665 @default.
• W2000212768 cites W2082250659 @default.
• W2000212768 cites W2086724237 @default.
• W2000212768 cites W2087310771 @default.
• W2000212768 cites W2098690863 @default.
• W2000212768 cites W2104828227 @default.
• W2000212768 cites W2105953403 @default.
• W2000212768 cites W2112361372 @default.
• W2000212768 cites W2130510436 @default.
• W2000212768 cites W2130756784 @default.
• W2000212768 cites W2132262862 @default.
• W2000212768 cites W2135699012 @default.
• W2000212768 cites W2144455554 @default.
• W2000212768 cites W2147281621 @default.
• W2000212768 cites W2147289825 @default.
• W2000212768 cites W2149412079 @default.
• W2000212768 cites W2151277682 @default.
• W2000212768 cites W2162059210 @default.
• W2000212768 cites W2167052843 @default.
• W2000212768 cites W2169246184 @default.
• W2000212768 cites W2171903383 @default.
• W2000212768 cites W4211084475 @default.
• W2000212768 doi "https://doi.org/10.1002/btpr.1556" @default.
• W2000212768 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22581577" @default.
• W2000212768 hasPublicationYear "2012" @default.
• W2000212768 type Work @default.
• W2000212768 sameAs 2000212768 @default.
• W2000212768 citedByCount "19" @default.
• W2000212768 countsByYear W20002127682012 @default.
• W2000212768 countsByYear W20002127682013 @default.
• W2000212768 countsByYear W20002127682014 @default.
• W2000212768 countsByYear W20002127682015 @default.
• W2000212768 countsByYear W20002127682016 @default.
• W2000212768 countsByYear W20002127682018 @default.
• W2000212768 countsByYear W20002127682019 @default.
• W2000212768 countsByYear W20002127682020 @default.
• W2000212768 countsByYear W20002127682023 @default.
• W2000212768 crossrefType "journal-article" @default.
• W2000212768 hasAuthorship W2000212768A5002850502 @default.
• W2000212768 hasAuthorship W2000212768A5006781936 @default.
• W2000212768 hasAuthorship W2000212768A5018500712 @default.
• W2000212768 hasAuthorship W2000212768A5029926741 @default.
• W2000212768 hasAuthorship W2000212768A5044324950 @default.
• W2000212768 hasAuthorship W2000212768A5059704494 @default.
• W2000212768 hasAuthorship W2000212768A5073662332 @default.
• W2000212768 hasAuthorship W2000212768A5076488840 @default.
• W2000212768 hasAuthorship W2000212768A5083066637 @default.
• W2000212768 hasConcept C104317684 @default.
• W2000212768 hasConcept C142724271 @default.
• W2000212768 hasConcept C153911025 @default.
• W2000212768 hasConcept C185592680 @default.
• W2000212768 hasConcept C190062978 @default.
• W2000212768 hasConcept C201663137 @default.
• W2000212768 hasConcept C204328495 @default.
• W2000212768 hasConcept C2775962898 @default.
• W2000212768 hasConcept C46522908 @default.
• W2000212768 hasConcept C547475151 @default.
• W2000212768 hasConcept C55493867 @default.
• W2000212768 hasConcept C71924100 @default.
• W2000212768 hasConcept C85755625 @default.
• W2000212768 hasConcept C86803240 @default.
• W2000212768 hasConcept C87190427 @default.
• W2000212768 hasConcept C95444343 @default.
• W2000212768 hasConceptScore W2000212768C104317684 @default.
• W2000212768 hasConceptScore W2000212768C142724271 @default.
• W2000212768 hasConceptScore W2000212768C153911025 @default.

• next