Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000216908> ?p ?o ?g. }
- W2000216908 endingPage "3090" @default.
- W2000216908 startingPage "3082" @default.
- W2000216908 abstract "ABSTRACT The widespread development of multidrug-resistant bacteria is a major health emergency. Conjugative DNA plasmids, which harbor a wide range of antibiotic resistance genes, also encode the protein factors necessary to orchestrate the propagation of plasmid DNA between bacterial cells through conjugative transfer. Successful conjugative DNA transfer depends on key catalytic components to nick one strand of the duplex DNA plasmid and separate the DNA strands while cell-to-cell transfer occurs. The TraI protein from the conjugative Salmonella plasmid pCU1 fulfills these key catalytic roles, as it contains both single-stranded DNA-nicking relaxase and ATP-dependent helicase domains within a single, 1,078-residue polypeptide. In this work, we unraveled the helicase determinants of Salmonella pCU1 TraI through DNA binding, ATPase, and DNA strand separation assays. TraI binds DNA substrates with high affinity in a manner influenced by nucleic acid length and the presence of a DNA hairpin structure adjacent to the nick site. TraI selectively hydrolyzes ATP, and mutations in conserved helicase motifs eliminate ATPase activity. Surprisingly, the absence of a relatively short (144-residue) domain at the extreme C terminus of the protein severely diminishes ATP-dependent strand separation. Collectively, these data define the helicase motifs of the conjugative factor TraI from Salmonella pCU1 and reveal a previously uncharacterized C-terminal functional domain that uncouples ATP hydrolysis from strand separation activity." @default.
- W2000216908 created "2016-06-24" @default.
- W2000216908 creator A5001420568 @default.
- W2000216908 creator A5013118750 @default.
- W2000216908 creator A5034343916 @default.
- W2000216908 date "2014-09-01" @default.
- W2000216908 modified "2023-10-16" @default.
- W2000216908 title "Unique Helicase Determinants in the Essential Conjugative TraI Factor from Salmonella enterica Serovar Typhimurium Plasmid pCU1" @default.
- W2000216908 cites W1541298097 @default.
- W2000216908 cites W1549419565 @default.
- W2000216908 cites W1576279956 @default.
- W2000216908 cites W1973102879 @default.
- W2000216908 cites W1977057342 @default.
- W2000216908 cites W1977504422 @default.
- W2000216908 cites W1977935196 @default.
- W2000216908 cites W1983195351 @default.
- W2000216908 cites W1984551289 @default.
- W2000216908 cites W1989565411 @default.
- W2000216908 cites W1998147205 @default.
- W2000216908 cites W1998467050 @default.
- W2000216908 cites W1999667822 @default.
- W2000216908 cites W2000152285 @default.
- W2000216908 cites W2003269199 @default.
- W2000216908 cites W2006220727 @default.
- W2000216908 cites W2009487096 @default.
- W2000216908 cites W2010292521 @default.
- W2000216908 cites W2015409494 @default.
- W2000216908 cites W2019405166 @default.
- W2000216908 cites W2024389323 @default.
- W2000216908 cites W2027076513 @default.
- W2000216908 cites W2027289172 @default.
- W2000216908 cites W2029987289 @default.
- W2000216908 cites W2038846224 @default.
- W2000216908 cites W2046298637 @default.
- W2000216908 cites W2051922054 @default.
- W2000216908 cites W2057259693 @default.
- W2000216908 cites W2058952776 @default.
- W2000216908 cites W2059271363 @default.
- W2000216908 cites W2060259273 @default.
- W2000216908 cites W2061522909 @default.
- W2000216908 cites W2063097994 @default.
- W2000216908 cites W2065677948 @default.
- W2000216908 cites W2083274165 @default.
- W2000216908 cites W2092296193 @default.
- W2000216908 cites W2094266696 @default.
- W2000216908 cites W2102715326 @default.
- W2000216908 cites W2105439835 @default.
- W2000216908 cites W2118182552 @default.
- W2000216908 cites W2119587006 @default.
- W2000216908 cites W2121438595 @default.
- W2000216908 cites W2122161002 @default.
- W2000216908 cites W2131352966 @default.
- W2000216908 cites W2132748660 @default.
- W2000216908 cites W2136549491 @default.
- W2000216908 cites W2136549510 @default.
- W2000216908 cites W2138424273 @default.
- W2000216908 cites W2150775872 @default.
- W2000216908 cites W2151888776 @default.
- W2000216908 cites W2152766682 @default.
- W2000216908 cites W2152959882 @default.
- W2000216908 cites W2157909266 @default.
- W2000216908 cites W2162384048 @default.
- W2000216908 cites W2164269384 @default.
- W2000216908 cites W2165613558 @default.
- W2000216908 cites W2166477912 @default.
- W2000216908 cites W2171526214 @default.
- W2000216908 cites W2176684272 @default.
- W2000216908 cites W2269237057 @default.
- W2000216908 doi "https://doi.org/10.1128/jb.01496-14" @default.
- W2000216908 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4135661" @default.
- W2000216908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24936053" @default.
- W2000216908 hasPublicationYear "2014" @default.
- W2000216908 type Work @default.
- W2000216908 sameAs 2000216908 @default.
- W2000216908 citedByCount "0" @default.
- W2000216908 crossrefType "journal-article" @default.
- W2000216908 hasAuthorship W2000216908A5001420568 @default.
- W2000216908 hasAuthorship W2000216908A5013118750 @default.
- W2000216908 hasAuthorship W2000216908A5034343916 @default.
- W2000216908 hasBestOaLocation W20002169081 @default.
- W2000216908 hasConcept C103244968 @default.
- W2000216908 hasConcept C104317684 @default.
- W2000216908 hasConcept C141315368 @default.
- W2000216908 hasConcept C153911025 @default.
- W2000216908 hasConcept C160136775 @default.
- W2000216908 hasConcept C161223559 @default.
- W2000216908 hasConcept C181199279 @default.
- W2000216908 hasConcept C204241405 @default.
- W2000216908 hasConcept C22744801 @default.
- W2000216908 hasConcept C23265538 @default.
- W2000216908 hasConcept C2776162087 @default.
- W2000216908 hasConcept C552990157 @default.
- W2000216908 hasConcept C55493867 @default.
- W2000216908 hasConcept C67705224 @default.
- W2000216908 hasConcept C86803240 @default.
- W2000216908 hasConcept C95444343 @default.
- W2000216908 hasConceptScore W2000216908C103244968 @default.
- W2000216908 hasConceptScore W2000216908C104317684 @default.