Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000220699> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2000220699 abstract "A variant Chinese hamster cell line has been isolated from a mutagenized population that has a markedly reduced ability to oxidize a variety of substrates via the Krebs cycle. The production of 14CO2 from 14C-labeled compounds was measured using pyruvate, acetate, beta-hydroxybutyrate, palmitate and glutamate, and in all cases it was neglibible in the mutant. In contrast to this, significant amounts of 14CO2 were produced from 14C-aspartate and 14C-succinate which suggest that some reactions of the Krebs cycle can take place and this conclusion is supported by tracer experiments with labeled compounds. The rate of respiration measured with a Clark oxygen electrode in the mutant was compared to several normal Chinese hamster cell lines and was found to be only 8%. Mitochondria appear to be present in normal numbers and with only minor differences in morphology. The measurement of difference spectra between oxidized and reduced states permits us to conclude that the cytochromes are all present and functional. These results lead us to believe that there may be a defect in the Krebs cycle between alpha-ketoglutarate and succinate. Alternatively a defect in a structural component of the mitochondria or in the electron-transport chain itself may be causing pleiotropic effects in the Krebs cycle and respiration." @default.
- W2000220699 created "2016-06-24" @default.
- W2000220699 creator A5017704257 @default.
- W2000220699 creator A5024361021 @default.
- W2000220699 creator A5091173366 @default.
- W2000220699 date "1975-04-01" @default.
- W2000220699 modified "2023-09-23" @default.
- W2000220699 title "Conditionally lethal mutations in Chinese hamster cells. Characterization of a cell line with a possible defect in the krebs cycle" @default.
- W2000220699 cites W1561167748 @default.
- W2000220699 cites W2009591404 @default.
- W2000220699 cites W2031618463 @default.
- W2000220699 cites W2053654911 @default.
- W2000220699 cites W2058962213 @default.
- W2000220699 cites W2071438950 @default.
- W2000220699 cites W2083885649 @default.
- W2000220699 cites W2130190854 @default.
- W2000220699 cites W2179838121 @default.
- W2000220699 cites W2334820938 @default.
- W2000220699 cites W2414038809 @default.
- W2000220699 cites W2494610378 @default.
- W2000220699 cites W2496366224 @default.
- W2000220699 cites W2897745919 @default.
- W2000220699 cites W43308639 @default.
- W2000220699 doi "https://doi.org/10.1002/jcp.1040850216" @default.
- W2000220699 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1123408" @default.
- W2000220699 hasPublicationYear "1975" @default.
- W2000220699 type Work @default.
- W2000220699 sameAs 2000220699 @default.
- W2000220699 citedByCount "44" @default.
- W2000220699 countsByYear W20002206992012 @default.
- W2000220699 countsByYear W20002206992013 @default.
- W2000220699 crossrefType "journal-article" @default.
- W2000220699 hasAuthorship W2000220699A5017704257 @default.
- W2000220699 hasAuthorship W2000220699A5024361021 @default.
- W2000220699 hasAuthorship W2000220699A5091173366 @default.
- W2000220699 hasConcept C104317684 @default.
- W2000220699 hasConcept C143065580 @default.
- W2000220699 hasConcept C144024400 @default.
- W2000220699 hasConcept C1491633281 @default.
- W2000220699 hasConcept C149923435 @default.
- W2000220699 hasConcept C153911025 @default.
- W2000220699 hasConcept C165135838 @default.
- W2000220699 hasConcept C182215343 @default.
- W2000220699 hasConcept C185592680 @default.
- W2000220699 hasConcept C202751555 @default.
- W2000220699 hasConcept C2777755551 @default.
- W2000220699 hasConcept C2778575167 @default.
- W2000220699 hasConcept C28859421 @default.
- W2000220699 hasConcept C2908647359 @default.
- W2000220699 hasConcept C29537977 @default.
- W2000220699 hasConcept C54355233 @default.
- W2000220699 hasConcept C55493867 @default.
- W2000220699 hasConcept C59822182 @default.
- W2000220699 hasConcept C62231903 @default.
- W2000220699 hasConcept C66517615 @default.
- W2000220699 hasConcept C81885089 @default.
- W2000220699 hasConcept C86803240 @default.
- W2000220699 hasConceptScore W2000220699C104317684 @default.
- W2000220699 hasConceptScore W2000220699C143065580 @default.
- W2000220699 hasConceptScore W2000220699C144024400 @default.
- W2000220699 hasConceptScore W2000220699C1491633281 @default.
- W2000220699 hasConceptScore W2000220699C149923435 @default.
- W2000220699 hasConceptScore W2000220699C153911025 @default.
- W2000220699 hasConceptScore W2000220699C165135838 @default.
- W2000220699 hasConceptScore W2000220699C182215343 @default.
- W2000220699 hasConceptScore W2000220699C185592680 @default.
- W2000220699 hasConceptScore W2000220699C202751555 @default.
- W2000220699 hasConceptScore W2000220699C2777755551 @default.
- W2000220699 hasConceptScore W2000220699C2778575167 @default.
- W2000220699 hasConceptScore W2000220699C28859421 @default.
- W2000220699 hasConceptScore W2000220699C2908647359 @default.
- W2000220699 hasConceptScore W2000220699C29537977 @default.
- W2000220699 hasConceptScore W2000220699C54355233 @default.
- W2000220699 hasConceptScore W2000220699C55493867 @default.
- W2000220699 hasConceptScore W2000220699C59822182 @default.
- W2000220699 hasConceptScore W2000220699C62231903 @default.
- W2000220699 hasConceptScore W2000220699C66517615 @default.
- W2000220699 hasConceptScore W2000220699C81885089 @default.
- W2000220699 hasConceptScore W2000220699C86803240 @default.
- W2000220699 hasLocation W20002206991 @default.
- W2000220699 hasLocation W20002206992 @default.
- W2000220699 hasOpenAccess W2000220699 @default.
- W2000220699 hasPrimaryLocation W20002206991 @default.
- W2000220699 hasRelatedWork W1972022817 @default.
- W2000220699 hasRelatedWork W2000220699 @default.
- W2000220699 hasRelatedWork W2008584431 @default.
- W2000220699 hasRelatedWork W2029691435 @default.
- W2000220699 hasRelatedWork W2061269540 @default.
- W2000220699 hasRelatedWork W2073096564 @default.
- W2000220699 hasRelatedWork W2097737837 @default.
- W2000220699 hasRelatedWork W2157921288 @default.
- W2000220699 hasRelatedWork W2394740058 @default.
- W2000220699 hasRelatedWork W2412364294 @default.
- W2000220699 isParatext "false" @default.
- W2000220699 isRetracted "false" @default.
- W2000220699 magId "2000220699" @default.
- W2000220699 workType "article" @default.