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- W2000228336 abstract "The composite of the actin cytoskeleton and plasma membrane plays important roles in many biological events. Here, we employed the emulsion method to synthesize artificial cells with biomimetic actin cortex in vesicles and characterized their mechanical properties. We demonstrated that the emulsion method provides the flexibility to adjust the lipid composition and protein concentrations in artificial cells to achieve the desired size distribution, internal microstructure, and mechanical properties. Moreover, comparison of the cortical elasticity measured for reconstituted artificial cells to that of real cells, including those manipulated using genetic depletion and pharmacological inhibition, strongly supports that actin cytoskeletal proteins are dominant over lipid molecules in cortical mechanics. Our study indicates that the assembly of biological systems in artificial cells with purified cellular components provides a powerful way to answer biological questions." @default.
- W2000228336 created "2016-06-24" @default.
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- W2000228336 date "2014-04-14" @default.
- W2000228336 modified "2023-09-27" @default.
- W2000228336 title "Mimicking the mechanical properties of the cell cortex by the self-assembly of an actin cortex in vesicles" @default.
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- W2000228336 doi "https://doi.org/10.1063/1.4871861" @default.
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