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• W2000231468 abstract "Recombinant polypeptides and protein domains containing two cysteine pairs located distal in primary sequence but proximal in the native folded or assembled state are labeled selectively in vitro and in mammalian cells using the profluorescent biarsenical reagents FlAsH-EDT2 and ReAsH-EDT2. This strategy, termed bipartite tetracysteine display, enables the detection of protein-protein interactions and alternative protein conformations in live cells. As proof of principle, we show that the equilibrium stability and fluorescence intensity of polypeptide–biarsenical complexes correlates with the thermodynamic stability of the protein fold or assembly. Destabilized protein variants form less stable and less bright biarsenical complexes, which allows discrimination of live cells expressing folded polypeptide and protein domains from those containing disruptive point mutations. Bipartite tetracysteine display may provide a means to detect early protein misfolding events associated with Alzheimer's disease, Parkinson's disease and cystic fibrosis; it may also enable high-throughput screening of compounds that stabilize discrete protein folds." @default.
• W2000231468 created "2016-06-24" @default.
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• W2000231468 date "2007-11-04" @default.
• W2000231468 modified "2023-10-17" @default.
• W2000231468 title "Surveying polypeptide and protein domain conformation and association with FlAsH and ReAsH" @default.
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• W2000231468 doi "https://doi.org/10.1038/nchembio.2007.49" @default.
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