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• W2000232541 abstract "During DNA replication, DNA polymerases alternate between DNA synthesis and proofreading the newly synthesized DNA. In order to understand the molecular details of how DNA polymerases determine the balance between polymerase and proofreading activities, it would be useful to have mutants which switch between the two activities either more or less frequently. Antimutator DNA polymerases switch more frequently and thus have more opportunity for proofreading. We have observed that mutant DNA polymerases which proofread less frequently have a mutator phenotype and are inhibited by the pyrophosphate analogue phosphonoacetic acid. Sensitivity to phosphonoacetic acid can be used to isolate second-site suppressor mutations. These suppressor mutations encode amino acid substitutions which produce antimutator DNA polymerases." @default.
• W2000232541 created "2016-06-24" @default.
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• W2000232541 date "1996-02-01" @default.
• W2000232541 modified "2023-10-01" @default.
• W2000232541 title "Selection of bacteriophage T4 antimutator DNA polymerases: a link between proofreading and sensitivity to phosphonoacetic acid" @default.
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• W2000232541 doi "https://doi.org/10.1016/0027-5107(95)00085-2" @default.
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