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- W2000238508 abstract "Abstract We have performed structural studies of four reiteration mutant genomes of SV40 (simian virus 40) using electron microscopic heteroduplex analysis, DNA-DNA reassociation tests, and analysis of restriction endonuclease cleavage patterns. Two reiteration mutants which appeared early during serial undiluted passages of two independent SV40 stocks were found to contain similar regions of the SV40 genome in their respective monomer fragments and no monkey substitution sequences. This observation is consistent with the presence of preferred sites for intramolecular recombination in the wildtype SV40 genome. Another reiteration mutant was shown to have a 530 base pair segment of the SV40 genome encompassing the origin for DNA replication which was linked to a 350 base pair monkey DNA sequence. Reassociation kinetic studies revealed that this monkey sequence was reiterated 10 to 20 times per diploid genome. All of the reiteration mutants studied preserved and amplified the origin for SV40 DNA replication." @default.
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- W2000238508 date "1977-03-01" @default.
- W2000238508 modified "2023-09-23" @default.
- W2000238508 title "Genome structures of reiteration mutants of simian virus 40" @default.
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- W2000238508 doi "https://doi.org/10.1016/0042-6822(77)90411-1" @default.
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