FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000239641> ?p ?o ?g. }

• W2000239641 endingPage "157" @default.
• W2000239641 startingPage "151" @default.
• W2000239641 abstract "A reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of rotavirus. Four primers were designed to recognize six distinct regions based on a highly conserved sequence in the VP7 region of the rotavirus genome. The optimal conditions for the LAMP assay were determined to 62 °C for 60 min with 8.0 mM MgSO4, 0.2 M betaine, 0.8 mM dNTPs, 0.2 μM each of outer primer, 1.6 μM each of inner primer. After amplification the products were detected either by observing a ladder pattern following gel electrophoresis, observation of turbidity, or a color change with the addition of SYBR Green I to the reaction tube. A ladder pattern of bands after gel electrophoresis was observed for only rotavirus isolates and showed that the rotavirus RT-LAMP assay was highly specific without any cross-reactivity with norovirus and astrovirus. The RT-LAMP assay was evaluated further using 79 fecal samples and 32 artificially seeded oyster samples. The detection limit of the RT-LAMP assay was 0.5 pg of rotavirus RNA. The sensitivity and simplicity of the test can served as a rapid and economic method for routine monitoring and risk assessment from clinical samples or marine products in both field conditions and laboratory settings." @default.
• W2000239641 created "2016-06-24" @default.
• W2000239641 creator A5002877468 @default.
• W2000239641 creator A5059606282 @default.
• W2000239641 creator A5063619990 @default.
• W2000239641 creator A5075909798 @default.
• W2000239641 creator A5084803961 @default.
• W2000239641 date "2014-07-01" @default.
• W2000239641 modified "2023-09-25" @default.
• W2000239641 title "Development and application of a loop-mediated isothermal amplification assay on rapid and sensitive detection of rotavirus in fecal samples and artificially seeded oysters" @default.
• W2000239641 cites W1969821154 @default.
• W2000239641 cites W1975801865 @default.
• W2000239641 cites W1979974453 @default.
• W2000239641 cites W1982583549 @default.
• W2000239641 cites W1988633002 @default.
• W2000239641 cites W1989776535 @default.
• W2000239641 cites W1996767092 @default.
• W2000239641 cites W2000914967 @default.
• W2000239641 cites W2005687294 @default.
• W2000239641 cites W2006763045 @default.
• W2000239641 cites W2014577159 @default.
• W2000239641 cites W2031665927 @default.
• W2000239641 cites W2032118018 @default.
• W2000239641 cites W2038099454 @default.
• W2000239641 cites W2051532481 @default.
• W2000239641 cites W2057876450 @default.
• W2000239641 cites W2083047717 @default.
• W2000239641 cites W2105275554 @default.
• W2000239641 cites W2109517773 @default.
• W2000239641 cites W2113200371 @default.
• W2000239641 cites W2113692311 @default.
• W2000239641 cites W2123497124 @default.
• W2000239641 cites W2127653958 @default.
• W2000239641 cites W2128398565 @default.
• W2000239641 cites W2147477994 @default.
• W2000239641 cites W3191903706 @default.
• W2000239641 doi "https://doi.org/10.1016/j.foodcont.2014.01.013" @default.
• W2000239641 hasPublicationYear "2014" @default.
• W2000239641 type Work @default.
• W2000239641 sameAs 2000239641 @default.
• W2000239641 citedByCount "4" @default.
• W2000239641 countsByYear W20002396412019 @default.
• W2000239641 countsByYear W20002396412020 @default.
• W2000239641 countsByYear W20002396412021 @default.
• W2000239641 crossrefType "journal-article" @default.
• W2000239641 hasAuthorship W2000239641A5002877468 @default.
• W2000239641 hasAuthorship W2000239641A5059606282 @default.
• W2000239641 hasAuthorship W2000239641A5063619990 @default.
• W2000239641 hasAuthorship W2000239641A5075909798 @default.
• W2000239641 hasAuthorship W2000239641A5084803961 @default.
• W2000239641 hasConcept C104317684 @default.
• W2000239641 hasConcept C119128265 @default.
• W2000239641 hasConcept C153911025 @default.
• W2000239641 hasConcept C159047783 @default.
• W2000239641 hasConcept C178790620 @default.
• W2000239641 hasConcept C185592680 @default.
• W2000239641 hasConcept C2522874641 @default.
• W2000239641 hasConcept C2777563447 @default.
• W2000239641 hasConcept C2780968160 @default.
• W2000239641 hasConcept C2781367151 @default.
• W2000239641 hasConcept C43617362 @default.
• W2000239641 hasConcept C49105822 @default.
• W2000239641 hasConcept C54355233 @default.
• W2000239641 hasConcept C552990157 @default.
• W2000239641 hasConcept C60635243 @default.
• W2000239641 hasConcept C86803240 @default.
• W2000239641 hasConceptScore W2000239641C104317684 @default.
• W2000239641 hasConceptScore W2000239641C119128265 @default.
• W2000239641 hasConceptScore W2000239641C153911025 @default.
• W2000239641 hasConceptScore W2000239641C159047783 @default.
• W2000239641 hasConceptScore W2000239641C178790620 @default.
• W2000239641 hasConceptScore W2000239641C185592680 @default.
• W2000239641 hasConceptScore W2000239641C2522874641 @default.
• W2000239641 hasConceptScore W2000239641C2777563447 @default.
• W2000239641 hasConceptScore W2000239641C2780968160 @default.
• W2000239641 hasConceptScore W2000239641C2781367151 @default.
• W2000239641 hasConceptScore W2000239641C43617362 @default.
• W2000239641 hasConceptScore W2000239641C49105822 @default.
• W2000239641 hasConceptScore W2000239641C54355233 @default.
• W2000239641 hasConceptScore W2000239641C552990157 @default.
• W2000239641 hasConceptScore W2000239641C60635243 @default.
• W2000239641 hasConceptScore W2000239641C86803240 @default.
• W2000239641 hasFunder F4320321001 @default.
• W2000239641 hasLocation W20002396411 @default.
• W2000239641 hasOpenAccess W2000239641 @default.
• W2000239641 hasPrimaryLocation W20002396411 @default.
• W2000239641 hasRelatedWork W1487857142 @default.
• W2000239641 hasRelatedWork W2060052075 @default.
• W2000239641 hasRelatedWork W2067557457 @default.
• W2000239641 hasRelatedWork W2090664738 @default.
• W2000239641 hasRelatedWork W2215050578 @default.
• W2000239641 hasRelatedWork W2352014247 @default.
• W2000239641 hasRelatedWork W2362411261 @default.
• W2000239641 hasRelatedWork W2566219082 @default.
• W2000239641 hasRelatedWork W2911770651 @default.
• W2000239641 hasRelatedWork W2919056727 @default.
• W2000239641 hasVolume "41" @default.
• W2000239641 isParatext "false" @default.

• next