Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000242509> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2000242509 endingPage "149" @default.
- W2000242509 startingPage "147" @default.
- W2000242509 abstract "Injection of antigenic peptides has been widely used as a vaccine strategy to boost T cell immunity. However, the poor immunogenicity of single peptides can potentially be strengthened through modification of the tertiary structure and the selection of the accompanying adjuvant. Here, we generated antigenic peptides into non-linear trimers by solid phase peptide synthesis, thereby enhancing antigen presentation by dendritic cells to CD8+ T cells in vitro and in vivo. CD8+ T cells from mice vaccinated with trimers showed an KLRG1+ effector phenotype and were able to recognize and kill antigen-expressing tumor cells ex vivo. Importantly, trimers outperformed synthetic long peptide in terms of T cell response even when equal number of epitopes were used for immunization. To improve the synthesis of trimers containing difficult peptide sequences, we developed a novel small molecule that functions as conjugation platform for synthetic long peptides. This platform , termed Antigen MAtriX (AMAX) improved yield, purity and solubility of trimers over conventional solid phase synthesis strategies. AMAX outperformed synthetic long peptides in terms of both CD8+ and CD4+ T cell responses and allowed functionalization with DC-SIGN-binding carbohydrates for in vivo dendritic cell targeting strategies, boosting T cell responses even further. Moreover, we show that agonistic CD40 antibody combined with MF59 (AddaVax) emulsion synergistically improves the antigen-specific T cell response of the AMAX in vivo. Also, tumor-associated antigens and neo-antigens could be incorporated in AMAX for tumor-specific CD8+ T cell responses. Importantly, immunization with a mix of neoantigen AMAX could reduce tumor growth in a pre-clinical syngeneic mouse model. Hence, we provide pre-clinical support for the induction of effector CD8+ T cells through the adaptable AMAX platform as easy implementable peptidic vaccination strategy against any antigen of choice, including neoantigens for anti-tumor immunity." @default.
- W2000242509 created "2016-06-24" @default.
- W2000242509 creator A5006676745 @default.
- W2000242509 date "2002-01-01" @default.
- W2000242509 modified "2023-09-26" @default.
- W2000242509 title "Comments on the influence of the acetabular labrum on hip joint cartilage consolidation: a poroelastic finite element model" @default.
- W2000242509 cites W1921722373 @default.
- W2000242509 cites W1966835709 @default.
- W2000242509 cites W1970470890 @default.
- W2000242509 cites W1985603154 @default.
- W2000242509 cites W2004304953 @default.
- W2000242509 cites W2013435554 @default.
- W2000242509 cites W2016221939 @default.
- W2000242509 cites W2023115269 @default.
- W2000242509 cites W2037540886 @default.
- W2000242509 cites W2037596655 @default.
- W2000242509 cites W2058595788 @default.
- W2000242509 cites W2072886168 @default.
- W2000242509 cites W2081648111 @default.
- W2000242509 cites W2096401877 @default.
- W2000242509 cites W2104089748 @default.
- W2000242509 cites W2143367874 @default.
- W2000242509 doi "https://doi.org/10.1016/s0021-9290(01)00136-1" @default.
- W2000242509 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11747894" @default.
- W2000242509 hasPublicationYear "2002" @default.
- W2000242509 type Work @default.
- W2000242509 sameAs 2000242509 @default.
- W2000242509 citedByCount "4" @default.
- W2000242509 countsByYear W20002425092016 @default.
- W2000242509 countsByYear W20002425092022 @default.
- W2000242509 crossrefType "journal-article" @default.
- W2000242509 hasAuthorship W2000242509A5006676745 @default.
- W2000242509 hasConcept C147483822 @default.
- W2000242509 hasConcept C167672396 @default.
- W2000242509 hasConcept C185592680 @default.
- W2000242509 hasConcept C203014093 @default.
- W2000242509 hasConcept C2776090121 @default.
- W2000242509 hasConcept C2780868878 @default.
- W2000242509 hasConcept C67662055 @default.
- W2000242509 hasConcept C83464605 @default.
- W2000242509 hasConcept C86803240 @default.
- W2000242509 hasConcept C8891405 @default.
- W2000242509 hasConcept C95444343 @default.
- W2000242509 hasConceptScore W2000242509C147483822 @default.
- W2000242509 hasConceptScore W2000242509C167672396 @default.
- W2000242509 hasConceptScore W2000242509C185592680 @default.
- W2000242509 hasConceptScore W2000242509C203014093 @default.
- W2000242509 hasConceptScore W2000242509C2776090121 @default.
- W2000242509 hasConceptScore W2000242509C2780868878 @default.
- W2000242509 hasConceptScore W2000242509C67662055 @default.
- W2000242509 hasConceptScore W2000242509C83464605 @default.
- W2000242509 hasConceptScore W2000242509C86803240 @default.
- W2000242509 hasConceptScore W2000242509C8891405 @default.
- W2000242509 hasConceptScore W2000242509C95444343 @default.
- W2000242509 hasIssue "1" @default.
- W2000242509 hasLocation W20002425091 @default.
- W2000242509 hasLocation W20002425092 @default.
- W2000242509 hasOpenAccess W2000242509 @default.
- W2000242509 hasPrimaryLocation W20002425091 @default.
- W2000242509 hasRelatedWork W1481917790 @default.
- W2000242509 hasRelatedWork W1519040467 @default.
- W2000242509 hasRelatedWork W1974103061 @default.
- W2000242509 hasRelatedWork W2000941383 @default.
- W2000242509 hasRelatedWork W2028370002 @default.
- W2000242509 hasRelatedWork W2038503104 @default.
- W2000242509 hasRelatedWork W2060503948 @default.
- W2000242509 hasRelatedWork W2095387359 @default.
- W2000242509 hasRelatedWork W2888060141 @default.
- W2000242509 hasRelatedWork W39915895 @default.
- W2000242509 hasVolume "35" @default.
- W2000242509 isParatext "false" @default.
- W2000242509 isRetracted "false" @default.
- W2000242509 magId "2000242509" @default.
- W2000242509 workType "article" @default.