FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000256447> ?p ?o ?g. }

• W2000256447 abstract "Abstract Nα-acetyltransferases (Nats) possess a wide range of important biological functions. Their structures can vary according to the first two residues of their substrate. However, the mechanisms of substrate recognition and catalysis of Nats are elusive. Here, we present two structure of Sulfolobus solfataricus Ard1 (SsArd1), a member of the NatA family, at 2.13 and 1.84 Å. Both structures contain coenzyme A, while the latter also contains a substrate-derived peptide. Sequential structure-based mutagenesis revealed that mutations of critical residues for CoA binding decreased the binding affinity of SsArd1 by 3 ~ 7-fold. Superimposition of SsArd1 (NatA) with human Naa50p (NatE) showed significant differences in key residues of enzymes near the first amino-acid position of the substrate peptide (Glu35 for SsArd1 and Val29 for Naa50p). Further enzyme activity assays revealed that the substrate specificity of SsArd1 could be altered from SSGTPT to MEEKVG by a range of Glu35 mutants. These studies provide not only a molecular elucidation of substrate recognition and specificity for the NatA family, but also insight into how members of the NAT family distinguish between amino acids at the substrate N-terminus from the ancient monomeric archaeal Ard1." @default.
• W2000256447 created "2016-06-24" @default.
• W2000256447 creator A5047576624 @default.
• W2000256447 creator A5071674838 @default.
• W2000256447 date "2015-03-02" @default.
• W2000256447 modified "2023-10-18" @default.
• W2000256447 title "Structural Basis for Substrate-specific Acetylation of Nα-acetyltransferase Ard1 from Sulfolobus solfataricus" @default.
• W2000256447 cites W1539796472 @default.
• W2000256447 cites W158285355 @default.
• W2000256447 cites W1827163203 @default.
• W2000256447 cites W1967363545 @default.
• W2000256447 cites W1971579210 @default.
• W2000256447 cites W1979903329 @default.
• W2000256447 cites W1986191025 @default.
• W2000256447 cites W1987045539 @default.
• W2000256447 cites W1989851713 @default.
• W2000256447 cites W1997612055 @default.
• W2000256447 cites W2008206202 @default.
• W2000256447 cites W2008207297 @default.
• W2000256447 cites W2016299048 @default.
• W2000256447 cites W2025636361 @default.
• W2000256447 cites W2030517878 @default.
• W2000256447 cites W2031029779 @default.
• W2000256447 cites W2036874847 @default.
• W2000256447 cites W2038840577 @default.
• W2000256447 cites W2048885488 @default.
• W2000256447 cites W2051870213 @default.
• W2000256447 cites W2065002182 @default.
• W2000256447 cites W2065886910 @default.
• W2000256447 cites W2082702196 @default.
• W2000256447 cites W2083195949 @default.
• W2000256447 cites W2088060011 @default.
• W2000256447 cites W2093931104 @default.
• W2000256447 cites W2097039792 @default.
• W2000256447 cites W2098646827 @default.
• W2000256447 cites W2108684255 @default.
• W2000256447 cites W2111199460 @default.
• W2000256447 cites W2113369651 @default.
• W2000256447 cites W2118818078 @default.
• W2000256447 cites W2124026197 @default.
• W2000256447 cites W2154714625 @default.
• W2000256447 cites W2156791826 @default.
• W2000256447 cites W2180229411 @default.
• W2000256447 doi "https://doi.org/10.1038/srep08673" @default.
• W2000256447 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5390088" @default.
• W2000256447 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25728374" @default.
• W2000256447 hasPublicationYear "2015" @default.
• W2000256447 type Work @default.
• W2000256447 sameAs 2000256447 @default.
• W2000256447 citedByCount "21" @default.
• W2000256447 countsByYear W20002564472015 @default.
• W2000256447 countsByYear W20002564472016 @default.
• W2000256447 countsByYear W20002564472017 @default.
• W2000256447 countsByYear W20002564472018 @default.
• W2000256447 countsByYear W20002564472020 @default.
• W2000256447 countsByYear W20002564472021 @default.
• W2000256447 crossrefType "journal-article" @default.
• W2000256447 hasAuthorship W2000256447A5047576624 @default.
• W2000256447 hasAuthorship W2000256447A5071674838 @default.
• W2000256447 hasBestOaLocation W20002564471 @default.
• W2000256447 hasConcept C104317684 @default.
• W2000256447 hasConcept C119157956 @default.
• W2000256447 hasConcept C143065580 @default.
• W2000256447 hasConcept C16318435 @default.
• W2000256447 hasConcept C181199279 @default.
• W2000256447 hasConcept C185592680 @default.
• W2000256447 hasConcept C18903297 @default.
• W2000256447 hasConcept C2777289219 @default.
• W2000256447 hasConcept C2778644216 @default.
• W2000256447 hasConcept C2779281246 @default.
• W2000256447 hasConcept C2780631447 @default.
• W2000256447 hasConcept C2781287210 @default.
• W2000256447 hasConcept C27911776 @default.
• W2000256447 hasConcept C2910274206 @default.
• W2000256447 hasConcept C515207424 @default.
• W2000256447 hasConcept C550995028 @default.
• W2000256447 hasConcept C55493867 @default.
• W2000256447 hasConcept C71240020 @default.
• W2000256447 hasConcept C86803240 @default.
• W2000256447 hasConceptScore W2000256447C104317684 @default.
• W2000256447 hasConceptScore W2000256447C119157956 @default.
• W2000256447 hasConceptScore W2000256447C143065580 @default.
• W2000256447 hasConceptScore W2000256447C16318435 @default.
• W2000256447 hasConceptScore W2000256447C181199279 @default.
• W2000256447 hasConceptScore W2000256447C185592680 @default.
• W2000256447 hasConceptScore W2000256447C18903297 @default.
• W2000256447 hasConceptScore W2000256447C2777289219 @default.
• W2000256447 hasConceptScore W2000256447C2778644216 @default.
• W2000256447 hasConceptScore W2000256447C2779281246 @default.
• W2000256447 hasConceptScore W2000256447C2780631447 @default.
• W2000256447 hasConceptScore W2000256447C2781287210 @default.
• W2000256447 hasConceptScore W2000256447C27911776 @default.
• W2000256447 hasConceptScore W2000256447C2910274206 @default.
• W2000256447 hasConceptScore W2000256447C515207424 @default.
• W2000256447 hasConceptScore W2000256447C550995028 @default.
• W2000256447 hasConceptScore W2000256447C55493867 @default.
• W2000256447 hasConceptScore W2000256447C71240020 @default.
• W2000256447 hasConceptScore W2000256447C86803240 @default.
• W2000256447 hasIssue "1" @default.
• W2000256447 hasLocation W20002564471 @default.

• next