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- W2000256468 abstract "A simple, rapid and specific method for analysis of gliclazide in serum by a sensitive high-performance liquid chromatographic method is described. Only 100 microl of serum and a little sample work-up is required. A simple procedure of extraction by toluene followed by evaporation to dryness under a gentle stream of air and dissolving the dried residue in mobile was used. The gliclazide peak was separated from endogenous peaks on a C(8) column by a mobile phase of acetonitrile-water (45:55, v/v), pH 3. Gliclazide and internal standard (phenytoin) were eluted at 6.8 and 3.8 min, respectively. The limit of quantitation (LOQ) for gliclazide in serum was 75 ng/ml at 230 nm. The method was linear over the range of 75-10,000 ng/ml with r(2) of 0.999. Mean recovery for gliclazide and internal standard was 84.5 and 87%, respectively." @default.
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- W2000256468 date "2003-03-05" @default.
- W2000256468 modified "2023-10-09" @default.
- W2000256468 title "A simple and sensitive HPLC method for determination of gliclazide in human serum" @default.
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- W2000256468 doi "https://doi.org/10.1016/s1570-0232(02)00951-0" @default.
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