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- W2000259043 abstract "A cost-efficient preparative enzymatic sulfation of oligosaccharides has been developed. Starting from adenosine 3'5'-diphosphate (PAP), the sulfate donating and highly expensive cofactor 3'-phosphoadenosine-5'-phosphosulfate (PAPS, 1) can be regenerated by using a recombinant aryl sulfotransferase and p-nitrophenyl sulfate. This system averts product inhibition by PAP and can serve as a continuous spectrophotometric assay for the activity of any sulfotransferase enzyme." @default.
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- W2000259043 date "1999-09-17" @default.
- W2000259043 modified "2023-09-29" @default.
- W2000259043 title "Enzymatic Regeneration of 3′-Phosphoadenosine-5′-Phosphosulfate Using Aryl Sulfotransferase for the Preparative Enzymatic Synthesis of Sulfated Carbohydrates" @default.
- W2000259043 doi "https://doi.org/10.1002/(sici)1521-3773(19990917)38:18<2747::aid-anie2747>3.0.co;2-2" @default.
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